Journal of Pharmaceutical Research

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Wound Healing Activity of Ethyl Acetate Fraction of Barleria Noctiflora in Experimentally Induced Diabetic Rats


Affiliations
1 Department of Pharmacology, JKKMMRF's – Annai JKK Sampoorani Ammal College of Pharmacy, B. Komarapalyam, Namakkal, Tamilnadu, India
2 Department of Pharmaceutical Chemistry, JKKMMRF's – Annai JKK Sampoorani Ammal College of Pharmacy, B.Komarapalyam, Namakkal, Tamilnadu, India
 

Purpose:To evaluate the wound healing activity of ethyl acetate fraction of Barleria noctiflora against the STZ induced diabetic rats.

Approach: The ethyl acetate fraction of Barleria noctiflora (EAFBN) was evaluated for its wound healing activity in Streptozotocin induced diabetic rats using excision and incision wound models. In case of excision wound model, parameters like wound contraction and period of epithelialization was studied, while in incision wound model, tensile strength of the wound was measured. In incision and excision models, treatment with ointment containing 10% w/w and 5% w/w of EAFBN was done.

Findings: Findings: The excision wound area exhibited significant (P<0.01) closure of EAFBN 5% w/w and 10% w/w treated animals showed 96.6% and 95.8%, complete epithelialization was observed in same day of EAFBN (10% w/w) and standard drug. In the incision wound model treated with the topical application of EAFBN 5% w/w treated animals skin breaking strength was 290.83±3.0, EAFBN (10% w/w) and standard drug have shown more or less same significant skin breaking strength (342.5 ± 3.81 and 350 ± 2.88).

Originality: The current study is the first time evaluation of the wound healing activity of Barleria noctiflora ethyl acetate fraction on STZ induced diabetes in rat. The characterization and isolation of the dynamic principle of new compound require broad research in the future.

Conclusion: The outcome of present study indicates the usage of ethyl acetate fraction of Barleria noctiflora in the management of wound healing especially in diabetes.


Keywords

Wound Healing, Barleria Noctiflora, Streptozotocin, Diabetic Rats.
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  • Wound Healing Activity of Ethyl Acetate Fraction of Barleria Noctiflora in Experimentally Induced Diabetic Rats

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Authors

Suresh Arumugam
Department of Pharmacology, JKKMMRF's – Annai JKK Sampoorani Ammal College of Pharmacy, B. Komarapalyam, Namakkal, Tamilnadu, India
Senthil Kumar Natesan
Department of Pharmacology, JKKMMRF's – Annai JKK Sampoorani Ammal College of Pharmacy, B. Komarapalyam, Namakkal, Tamilnadu, India
Sekar Ganesan
Department of Pharmacology, JKKMMRF's – Annai JKK Sampoorani Ammal College of Pharmacy, B. Komarapalyam, Namakkal, Tamilnadu, India
Sumathi Kanagarajan
Department of Pharmaceutical Chemistry, JKKMMRF's – Annai JKK Sampoorani Ammal College of Pharmacy, B.Komarapalyam, Namakkal, Tamilnadu, India

Abstract


Purpose:To evaluate the wound healing activity of ethyl acetate fraction of Barleria noctiflora against the STZ induced diabetic rats.

Approach: The ethyl acetate fraction of Barleria noctiflora (EAFBN) was evaluated for its wound healing activity in Streptozotocin induced diabetic rats using excision and incision wound models. In case of excision wound model, parameters like wound contraction and period of epithelialization was studied, while in incision wound model, tensile strength of the wound was measured. In incision and excision models, treatment with ointment containing 10% w/w and 5% w/w of EAFBN was done.

Findings: Findings: The excision wound area exhibited significant (P<0.01) closure of EAFBN 5% w/w and 10% w/w treated animals showed 96.6% and 95.8%, complete epithelialization was observed in same day of EAFBN (10% w/w) and standard drug. In the incision wound model treated with the topical application of EAFBN 5% w/w treated animals skin breaking strength was 290.83±3.0, EAFBN (10% w/w) and standard drug have shown more or less same significant skin breaking strength (342.5 ± 3.81 and 350 ± 2.88).

Originality: The current study is the first time evaluation of the wound healing activity of Barleria noctiflora ethyl acetate fraction on STZ induced diabetes in rat. The characterization and isolation of the dynamic principle of new compound require broad research in the future.

Conclusion: The outcome of present study indicates the usage of ethyl acetate fraction of Barleria noctiflora in the management of wound healing especially in diabetes.


Keywords


Wound Healing, Barleria Noctiflora, Streptozotocin, Diabetic Rats.

References