Tissue Culture Studies on Shankhpushpi

Callus from ischolar_main portion of seedlings of Convolvulus pluricaulis has been cultured on a medium (BM) based on Murashige and Skoog's (1962) medium. Sucrose, 1.5%; casein hydrolysate, 1250 mg/l ; IAA, 15 mg/l ; kinetin 1.5 mg/l and GA, 0.5 mg/l, individually were found optimum for the maximum growth of callus.

Maximum hypotensive activity was noticed in the extract of callus tissue cultured on BM supplemented with a higher concentration of casein hydrolysate.