Journal of Ecophysiology and Occupational Health

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This study investigated phytochemical content, antimicrobial and plasmid curing property of four extracts (viz. petroleum ether, ethanol, distilled water and acetone) by reflux extraction technique of leaves of Aloe vera and Ocimum sanctum. Qualitative and quantitative analysis of secondary metabolites were carried out. Agar well diffusion method was used for antibacterial activity against Escherichia coli and Bacillus cereus and plasmid curing property of phytoextracts was carried out. The result revealed that the tested plant materials contained appreciable amounts of total alkaloids (16.00- 71.00 mg/gm leaves), total phenols (10.233-40.267 μg g-1 fw), total saponins (10.267-65.167 μg g-1 fw) zone of inhibition (0.00-12.33 mm) and plasmid curing property (81-84%). Generally higher extract yields, secondary metabolites, antimicrobial activity and plasmid curing property were obtained using ethanol as a solvents, as compared to the respective other solvents.

Keywords

Phytochemicals, Plasmid Curing, Reflux Extraction, Aloe vera, Ocimum sanctum, Agar well Diffusion, Escherichia coli and Bacillus cereus.
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