Journal of Ecophysiology and Occupational Health

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Evaluation of In Vitro Antioxidant Properties of Methanolic and Aqueous Extracts of Terminalia bellerica Roxb Leaves


Affiliations
1 Nutraceutical Laboratory, Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, India
2 Department of Biotechnology, IFTM University, Moradabad-244102, India
 

The antioxidant activity of methanolic (MTBL) and aqueous (ATBL) extracts of Terminalia bellerica leaves (TBL) was evaluated by various in vitro techniques. IC50 values of MTBL and ATBL were found to be 93 and 301 μg mL-1 for FRSA, 42 and 252 μg mL-1 for SARSA, 91 and 376 μg mL-1 for LPO, 53 and 540 μg mL-1 for HRSA, 142 and 544 μg mL-1 for FTC, respectively. RP was detected to be 2.72 and 10.49 ASE mL-1 for MTBL and ATBL. The MTBL showed better antioxidant activity than ATBL when compared with standard quercetin. TPC for MTBL and ATBL were 209.28 and 173.9 g kg−1 of GAE, respectively. In conclusion, methanolic extract of TB leaf has higher antioxidant activity than the aqueous extract and it could be a good source of natural antioxidant.

Keywords

Terminalia bellerica, Antioxidant, Total Phenolic Content.
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  • Evaluation of In Vitro Antioxidant Properties of Methanolic and Aqueous Extracts of Terminalia bellerica Roxb Leaves

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Authors

Rasna Gupta
Nutraceutical Laboratory, Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, India
Ram Lakhan Singh
Nutraceutical Laboratory, Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, India
Neeraja Dwivedi
Department of Biotechnology, IFTM University, Moradabad-244102, India

Abstract


The antioxidant activity of methanolic (MTBL) and aqueous (ATBL) extracts of Terminalia bellerica leaves (TBL) was evaluated by various in vitro techniques. IC50 values of MTBL and ATBL were found to be 93 and 301 μg mL-1 for FRSA, 42 and 252 μg mL-1 for SARSA, 91 and 376 μg mL-1 for LPO, 53 and 540 μg mL-1 for HRSA, 142 and 544 μg mL-1 for FTC, respectively. RP was detected to be 2.72 and 10.49 ASE mL-1 for MTBL and ATBL. The MTBL showed better antioxidant activity than ATBL when compared with standard quercetin. TPC for MTBL and ATBL were 209.28 and 173.9 g kg−1 of GAE, respectively. In conclusion, methanolic extract of TB leaf has higher antioxidant activity than the aqueous extract and it could be a good source of natural antioxidant.

Keywords


Terminalia bellerica, Antioxidant, Total Phenolic Content.

References





DOI: https://doi.org/10.15512/joeoh%2F2015%2Fv15i3-4%2F121596