Journal of Endocrinology and Reproduction

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Phytomelatonin (Zea mays) Supplementation Restores the Damage Caused by Induced-Diabetes in the Golden Hamster Mesocricetus auratus


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1 Pineal Research Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, India
     

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Diabetes is a lifestyle disorder with multiple etiologies, one of them being damage induced by free radicals. Melatonin, a neurohormone secreted by the pineal gland, is a well-known antioxidant or free radical scavenger. The melatonin found in plants is known as phytomelatonin. Phytomelatonin is potent in regulating stress management, apoptosis, seasonality and circadian rhythms in animals as melatonin. The supplementation of phytomelatonin is known to potentiate the antioxidant capacity. Therefore, in the present study, we proposed that the supplementation of corn seed (Zea mays) with regular diet may modulate the activity of antioxidative enzymes in diabetic hamsters. The supplementation of diet with phytomelatoninrich corn did not reduce serum glucose level significantly. No significant elevation was noted in serum insulin level of animals after feeding corn. Glycogen level of both liver and muscle were also not significantly affected. However, phytomelatonin supplementation improved lipid profile by significantly reducing the cholesterol (TC) and LDL Cholesterol (LDL-C) and enhancing HDL Cholesterol (HDL-C). Significant reduction was noted in LPO level in pancreas. The supplemental diet led to significant increase in the level of Super Oxide Dismutase (SOD) and catalase (CAT) in pancreas. Diabetes produced a deleterious effect on oxidative stress markers, lipid profile, glucose, glycogen and insulin. Supplementation of corn in the diet for 40 days modified the biochemical parameters to various degrees. The phytomelatonin treatment improved most of the antioxidant parameters under investigation. The study has produced some positive outcomes, especially a strategy which may be relevant in prevention, development and/or slowing down of the progression of diabetes.

Keywords

Diabetes, Hamster, Melatonin, Phytomelatonin, Zea mays
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  • Rochette L, Zeller M, Cottin Y, Vergely C. Diabetes, oxidative stress and therapeutic strategies. Biochimica et Biophysica Acta (BBA)-General Subjects. 2014; 1840(9):2709- 2729. https://doi.org/10.1016/j.bbagen.2014.05.017. PMid:24905298.

  • Motawi TK, Ahmed SA, Hamed MA, El-Maraghy SA, Aziz WM. Combination of melatonin and certain drugs for treatment of diabetic nephropathy in streptozotocininduced diabetes in rats. Diabetology International. 2016; 7(4):413-424. https://doi.org/10.1007/s13340-016-0268-9. PMid:30603294 PMCid:PMC6224965.

  • Tan DX, Hardeland R, Manchester LC, Korkmaz A, Ma S, Rosales-Corral S, Reiter RJ. Functional roles of melatonin in plants, and perspectives in nutritional and agricultural science. Journal of Experimental Botany. 2012; 63(2):577- 597. https://doi.org/10.1093/jxb/err256. PMid:22016420.

  • Hattori A, Migitaka H, Iigo M, Itoh M, Yamamoto K, Ohtani- Kaneko R, Hara M, Suzuki T, Reiter RJ. Identification of melatonin in plants and its effects on plasma melatonin levels and binding to melatonin receptors in vertebrates. Biochemistry and Molecular Biology International. 1995; 35(3):627-634.

  • Hardeland R, Pandi-Perumal SR. Melatonin, a potent agent in antioxidative defense: Actions as a natural food constituent, gastrointestinal factor, drug and prodrug. Nutrition and Metabolism. 2005; 2(1):22. https://doi. org/10.1186/1743-7075-2-22. PMid:16153306 PMCid: PMC1262766.

  • Reiter RJ, Manchester LC, Tan DX. Melatonin in walnuts: influence on levels of melatonin and total antioxidant capacity of blood. Nutrition. 2006; 21:920-924. https://doi. org/10.1016/j.nut.2005.02.005. PMid:15979282.

  • Maldonado MD, Moreno H, Calvo JR. Melatonin present in beer contributes to increase the levels of melatonin and antioxidant capacity of the human serum. Clinical Nutrition. 2009; 28(2):188-191. https://doi.org/10.1016/j. clnu.2009.02.001. PMid:19249143.

  • Singh AK, Haldar C. Supplementation of corn seed with regular diet modulates immune function and antioxidant status in Capra hircus. Journal of Animal Physiology and Animal Nutrition. 2017; 101(6):1205-1214. https://doi.org/10.1111/jpn.12641. PMid:28133830.

  • Das K, Samanta L, Chainy GBN. A modified spectrophotometric assay of superoxide dismutase using nitrite formation by superoxide radicals. Indian J. Exp. Biol. 2000; 37(3):201-204.

  • Sinha AK. Colorimetric assay of catalase. Analytical Biochemistry. 1972; 47(2):389-394. https://doi.org/10.1016/0003-2697(72)90132-7.

  • Ohkawa H, Ohishi N, Yagi K. Reaction of linoleic acid hydroperoxide with thiobarbituric acid. Journal of Lipid Research. 1978; 19(8):1053-1057. https://doi.org/10.1016/ S0022-2275(20)40690-X.

  • Bonomini F, Borsani E, Favero G, Rodella LF, Rezzani R. Dietary melatonin supplementation could be a promising preventing/therapeutic approach for a variety of liver diseases. Nutrients. 2018; 10(9):1135. https://doi.org/10.3390/nu10091135. PMid:30134592 PMCid:PMC6164189.


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  • Phytomelatonin (Zea mays) Supplementation Restores the Damage Caused by Induced-Diabetes in the Golden Hamster Mesocricetus auratus

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Authors

Sweta Arora
Pineal Research Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, India
Chandana Haldar
Pineal Research Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, India

Abstract


Diabetes is a lifestyle disorder with multiple etiologies, one of them being damage induced by free radicals. Melatonin, a neurohormone secreted by the pineal gland, is a well-known antioxidant or free radical scavenger. The melatonin found in plants is known as phytomelatonin. Phytomelatonin is potent in regulating stress management, apoptosis, seasonality and circadian rhythms in animals as melatonin. The supplementation of phytomelatonin is known to potentiate the antioxidant capacity. Therefore, in the present study, we proposed that the supplementation of corn seed (Zea mays) with regular diet may modulate the activity of antioxidative enzymes in diabetic hamsters. The supplementation of diet with phytomelatoninrich corn did not reduce serum glucose level significantly. No significant elevation was noted in serum insulin level of animals after feeding corn. Glycogen level of both liver and muscle were also not significantly affected. However, phytomelatonin supplementation improved lipid profile by significantly reducing the cholesterol (TC) and LDL Cholesterol (LDL-C) and enhancing HDL Cholesterol (HDL-C). Significant reduction was noted in LPO level in pancreas. The supplemental diet led to significant increase in the level of Super Oxide Dismutase (SOD) and catalase (CAT) in pancreas. Diabetes produced a deleterious effect on oxidative stress markers, lipid profile, glucose, glycogen and insulin. Supplementation of corn in the diet for 40 days modified the biochemical parameters to various degrees. The phytomelatonin treatment improved most of the antioxidant parameters under investigation. The study has produced some positive outcomes, especially a strategy which may be relevant in prevention, development and/or slowing down of the progression of diabetes.

Keywords


Diabetes, Hamster, Melatonin, Phytomelatonin, Zea mays

References





DOI: https://doi.org/10.18311/jer%2F2020%2F27401