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Pawar, Sachin S.
- HPTLC Method for the Simultaneous Estimation of Catechol, Caffeic Acid and Anthraquinone in the Leaf Extract of Xanthium Strumarium and Its Formulation
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International Journal of Innovative Research and Development, Vol 4, No 6 (2015), Pagination:Abstract
A simple, precise, accurate and rapid High-Performance Thin Layer Chromatographic method has been developed and validated for the simultaneous estimation of of catechol, caffeic acid and anthraquinone in the leaf extract of Xanthium strumarium and its Formulation. The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of n-hexane: chloroform: ethyl acetate: methanol (6:2:2:1 v/v/v/v). The detection of spots were carried out at 254 nm. This HPTLC method was validated statistically and recovery study was performed to confirm the accuracy of the method. It can be used for routine quality control of herbal raw materials as well as formulations containing any or all of these compounds.
A simple, precise, accurate and rapid High-Performance Thin Layer Chromatographic method has been developed and validated for the simultaneous estimation of of catechol, caffeic acid and anthraquinone in the leaf extract of Xanthium strumarium and its Formulation. The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of n-hexane: chloroform: ethyl acetate: methanol (6:2:2:1 v/v/v/v). The detection of spots were carried out at 254 nm. This HPTLC method was validated statistically and recovery study was performed to confirm the accuracy of the method. It can be used for routine quality control of herbal raw materials as well as formulations containing any or all of these compounds.
Keywords
Simultaneous estimation, HPTLC, Catechol, Caffeic acid and Anthraquinone- Simultaneous Quantification of P-Methoxybenzoic Acid, 3, 4-Dimethoxycinnamic Acid and Ecdysteron in the Extract of Trianthema Portulacastrum Linn. And Its Marketed Polyherbal Formulation Using HPLC
Authors
Source
International Journal of Innovative Research and Development, Vol 4, No 6 (2015), Pagination:Abstract
Trianthema portulacastrum Linn. belonging to the family aizoaceae is abundantly available weed which has enormous traditional uses due to different bioactive compounds present and thus used in different herbal formulations. A simple, reproducible and efficient reverse phase high performance liquid chromatographic method has been developed for simultaneous determination of p-methoxy benzoic acid, 3,4-dimethoxy cinnamic acid and Ecdysterone in the powder of Trianthema Portulacastrum. The Agiland 1100 system was used. The method involves use of a C18 column (Inertsil ODS , C18 15cm X 4.6 mm, 5µm) with a gradient mixture of acetonitrile and 0.01M NaH2PO4 as mobile phase. The developed method was then validated in accordance with the ICH guidelines for method validation. The validated HPLC method can be used for routine quality control analysis of formulations or dietary supplements containing leaf powder of Trianthema Portulacastrum Linn.
Trianthema portulacastrum Linn. belonging to the family aizoaceae is abundantly available weed which has enormous traditional uses due to different bioactive compounds present and thus used in different herbal formulations. A simple, reproducible and efficient reverse phase high performance liquid chromatographic method has been developed for simultaneous determination of p-methoxy benzoic acid, 3,4-dimethoxy cinnamic acid and Ecdysterone in the powder of Trianthema Portulacastrum. The Agiland 1100 system was used. The method involves use of a C18 column (Inertsil ODS , C18 15cm X 4.6 mm, 5µm) with a gradient mixture of acetonitrile and 0.01M NaH2PO4 as mobile phase. The developed method was then validated in accordance with the ICH guidelines for method validation. The validated HPLC method can be used for routine quality control analysis of formulations or dietary supplements containing leaf powder of Trianthema Portulacastrum Linn.