Simultaneous Quantification of P-Methoxybenzoic Acid, 3, 4-Dimethoxycinnamic Acid and Ecdysteron in the Extract of Trianthema Portulacastrum Linn. And Its Marketed Polyherbal Formulation Using HPLC

Trianthema portulacastrum Linn. belonging to the family aizoaceae is abundantly available weed which has enormous traditional uses due to different bioactive compounds present  and thus used in different herbal formulations. A simple, reproducible and efficient reverse phase high performance liquid chromatographic method has been developed for simultaneous determination of p-methoxy benzoic acid, 3,4-dimethoxy cinnamic acid and Ecdysterone in the powder of Trianthema Portulacastrum. The Agiland 1100 system was used. The method involves use of a C18 column (Inertsil ODS , C18 15cm X 4.6 mm, 5µm) with a gradient mixture of acetonitrile and 0.01M NaH2PO4 as mobile phase. The developed method was then validated in accordance with the ICH guidelines for method validation. The validated HPLC method can be used for routine quality control analysis of formulations or dietary supplements containing leaf powder of Trianthema Portulacastrum Linn.

Trianthema portulacastrum Linn. belonging to the family aizoaceae is abundantly available weed which has enormous traditional uses due to different bioactive compounds present  and thus used in different herbal formulations. A simple, reproducible and efficient reverse phase high performance liquid chromatographic method has been developed for simultaneous determination of p-methoxy benzoic acid, 3,4-dimethoxy cinnamic acid and Ecdysterone in the powder of Trianthema Portulacastrum. The Agiland 1100 system was used. The method involves use of a C18 column (Inertsil ODS , C18 15cm X 4.6 mm, 5µm) with a gradient mixture of acetonitrile and 0.01M NaH2PO4 as mobile phase. The developed method was then validated in accordance with the ICH guidelines for method validation. The validated HPLC method can be used for routine quality control analysis of formulations or dietary supplements containing leaf powder of Trianthema Portulacastrum Linn.