HPTLC Method for the Simultaneous Estimation of Catechol, Caffeic Acid and Anthraquinone in the Leaf Extract of Xanthium Strumarium and Its Formulation

A simple, precise, accurate and rapid High-Performance Thin Layer Chromatographic method has been developed and validated for the simultaneous estimation of of catechol, caffeic acid and  anthraquinone in the leaf extract of Xanthium strumarium and its Formulation. The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of n-hexane: chloroform: ethyl acetate: methanol (6:2:2:1 v/v/v/v). The detection of spots were carried out at 254 nm. This HPTLC method was validated statistically and recovery study was performed to confirm the accuracy of the method. It can be used for routine quality control of herbal raw materials as well as formulations containing any or all of these compounds.

A simple, precise, accurate and rapid High-Performance Thin Layer Chromatographic method has been developed and validated for the simultaneous estimation of of catechol, caffeic acid and  anthraquinone in the leaf extract of Xanthium strumarium and its Formulation. The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of n-hexane: chloroform: ethyl acetate: methanol (6:2:2:1 v/v/v/v). The detection of spots were carried out at 254 nm. This HPTLC method was validated statistically and recovery study was performed to confirm the accuracy of the method. It can be used for routine quality control of herbal raw materials as well as formulations containing any or all of these compounds.