Journal of Horticultural Sciences

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PGPR in Managing Root Rot Disease and Enhancing Growth in Mandarin (Citrus reticulata Blanco.) Seedlings


Affiliations
1 Immuno-Phytopathology Laboratory, Department of Botany, University of North Bengal, Siliguri, Darjeeling, West Bengal - 734 013, India
 

Decline in general plant-health and fruit production in mandarin influenced by abiotic and biotic factors is a major threat to cultivars grown in Darjeeling and Sikkim hills. Fusarium ischolar_main rot, caused by F. oxysporum, is one of the most serious diseases afflicted during early plant growth stage in Citrus. To address this, seven PGPR isolates - Pseudomonas poae (RMK03), Bacillus stratosphericus (RHS/CL-01), Ochrobactrum anthropi, Paenibacillus lentimorbus, Bacillus pumilus, Bacillus megaterium and Bacillus amyloliquefaciens were isolated from the rhizosphere of Citrus reticulata, C. limonia and Camellia sinensis, and used for evaluating their effect on growth of mandarin seedlings. Pseudomonas poae showed in vitro antagonism to Fusarium oxysporum. Better growth enhancement was noticed with P. poae, B. stratosphericus, O. anthropi and B. pumilus. Enhanced activity of chlorophyll, total protein, phenol, four major defense enzymeschitinase, β-1, 3-glucanase, peroxidase and phenyalanine ammonia lyase was observed upon application of PGPR. P. poae also suppressed ischolar_main rot caused by Fusarium oxysporum. Use of PGPR, which promote growth besides reducing disease severity to some extent, may lead to use of eco-friendly approaches for controlling plant diseases.

Keywords

PGPR, Mandarin, Root Rot, Citrus, Bacterial Isolates.
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  • Akhtar, M.S., Shakeel, U. and Siddiqui, Z.A. 2010. Biocontrol of Fusarium wilt by Bacillus pumilus, Pseudomonas alcaligenes and Rhizobium sp. on lentil. Tur. J. Biol., 34:1-7

  • Acharya, A., Chakraborty, U. and Chakraborty, B.N. 2013. Improvement of health status of Litsea monopetala using plant growth promoting rhizobacteria. Int’l. J. Bio-res. Stress Mgt., 4:187-191

  • Asadhi, S., Reddy, B.V.B., Sivaprasad, Y., Prathyusha, M., Krishna, T.M., Kumar, K.V.K. and Reddy, K.R. 2013. Characterization, genetic diversity and antagonistic potential of 2,4- diacetylphloroglucinol producing Pseudomonas fluorescens isolates in groundnut-based cropping systems of Andhra Pradesh, India. Arch. Phytopathol. Pl. Protect., 46:1966-1977

  • Barassi, C.A., Ayrault, G., Creus, C.M., Sueldo, R.J. and Sobrero, M.T. 2006. Seed inoculation with Azospirillum mitigates NaCl effects on lettuce. Sci. Hortic., 109:8-14

  • Bhattacharya, M.K. and Ward, E.W.B. 1987. Biosynthesis and metabolism of glyceollin I in soybean hypocotyls following wounding or inoculation with Phytophthora megasperma f. sp. glycinea. Physiol. Mol. Pl. Pathol., 31:401-409

  • Boller, T. and Mauch, F. 1998. Colorimetric assay for chitinase. Methods in Enzymol., 161:430-435

  • Buchanan, R.E. and Gibbson, N.E. 1974. Bergeys Manual of Determinative Bacteriology, Williams and Wilkins Co., Baltimore (8th ed.), 1246 p.

  • Cao, Y., Zhang, Z., Ling, N., Yuan, Y., Zheng, X., Shen, B. and Shen, Q. 2011. Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant ischolar_mains. Biol. Fertl. Soils., 47:495-506

  • Chabot, R., Antoun, H. and Cescas, M.P. 1996. Growth promotion of maize and lettuce by phosphate solubilizing Rhizobium leguminosarum biovar. Phaseoli. Pl. Soil, 184:311–321

  • Chakraborty, U. and Chakraborty, B.N. 1989. Interaction of Rhizobium leguminosarum and Fusarium solani f. sp. pisi on pea affecting disease development and phytoalexin production. Can. J. Bot., 67:1698-1701

  • Chakraborty, U., Chakraborty, B.N. and Chakraborty, A.P. 2010. Influence of Serratia marcescens TRS-1 on growth promotion and induction of resistance in Camellia sinensis against Fomes lamaoensis. J. Pl. Interact., 5:261-272

  • Chakraborty, U., Chakraborty, B.N. and Kapoor, M. 1993. Changes in the levels of peroxidase and phenyl alanine ammonia lyase in Brassica napus cultivars showing variable resistance to Leptosphaeria maculans. Folia Microbiologica, 38:491-496

  • Chakraborty, U., Chakraborty, B.N., Allay, S., De, U. and Chakraborty, A.P. 2011. Dual application of Bacillus pumilus and Glomus mosseae for improvement of health status of mandarin plants. Acta Hort., 892:215-229

  • Chakraborty, U., Chakraborty, B.N. and Basnet, M. 2006. Plant growth promotion and induction of resistance in Camellia sinensis by Bacillus megaterium. J. Basic Microbiol., 46:186-195

  • Chakraborty, U., Chakraborty, B.N., Basnet, M. and Chakraborty, A.P. 2009. Evaluation of Ochrobactrum anthropi TRS-2 and its talc based formulation for enhancement of growth of tea plants and management of brown ischolar_main rot disease. J. Appl. Microbiol., 107:625-634

  • Chakraborty, U., Chakraborty, B.N., Chakraborty, A.P., Sunar, K. and Dey, P.L. 2013. Plant growth promoting rhizobacteria mediated improvement of health status of tea plants. Indian J. Biotechnol., 12:20-31

  • Dakora, F.D. 2003. Defining new roles for plant and rhizobial molecules in sole and mixed plant cultures involving symbiotic legumes. New Phytologist, 158:39-49

  • Dobbelaere, S., Croonenberghs, A., Thys, A., Vande Broek, A. and Vanderleyden, J. 1999. Photostimulatory effects of Azospirillum brasilense wild type and mutant strain altered in IAA production on wheat. Pl. Soil, 212:155-164

  • Esitken, A. 2011. Use of Plant Growth Promoting Rhizobacteria in Horticultural Crops. In: Bacteria in Agrobiology: Crop Ecosystems. D.K. Maheshwari (ed.), pp. 189-235

  • Glick, B.R. 1995. The enhancement of plant growth by free-living bacteria. Can. J. Microbiol., 41:109-117

  • Han, H.S. and Lee, K.D. 2004. Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of lettuce under soil salinity. Res. J. Agril. Biol. Sci., 1:210-215

  • Harborne, J.B. 1973. Phytochemical Methods. Chapman and Hall Ltd, London. 278 p.

  • Jacobsen, C.S. 1997. Plant protection and rhizosphere colonization of barley by seed inoculated herbicide degrading Burkholderia (Pseudomonas) cepacia DBO1 (pRO101) in 2,4-D contaminated soil. Pl. Soil, 189:139-144

  • Karlidag, H., Esitken, A., Yildirim, E., Donmez, M.F. and Turan, M. 2011. Effects of plant growth promoting bacteria (PGPB) on yield, growth, leaf water content, membrane permeability and ionic composition of strawberry under saline conditions. J. Pl. Nutr., 34:34-45

  • Lavania, M., Chauhan, P.S., Chauhan, S.V., Singh, H.B. and Nautiyal, C.S. 2006. Induction of plant defense enzymes and phenolics by treatment with plant growth promoting rhizobacterial Serratia marcescens NBRII213. Curr. Microbiol., 52:363-368

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with folin phenol reagent. J. Biol. Chem., 193:265-275

  • Mahadevan, A. and Sridhar, R. 1982. Methods in Physiological Plant Pathology (2nd ed.), Sivakami Publications, Madras, Tamil Nadu, India.

  • Marulanda-Aguirre, A., Azcon, R., Ruiz-Lozano, J.M. and Aroca, R. 2008. Differential effects of a Bacillus megaterium strain on Lactuca sativa plant growth depending on the origin of the arbuscular mycorrhizal fungus coinoculated: physiologic and biochemical traits. J. Pl. Growth Regul., 27:10-18

  • Mayak, S., Tirosh, T. and Glick, B.R. 2004. Plant growth-promoting bacteria that confer resistance to water stress in tomatoes and peppers. Pl. Sci., 166:525-530

  • Mohamedy-El, R.S.R. and Ahmed, M.A. 2009. Effect of biofertilizers and humic acid on control of dry ischolar_main rot disease and improvement yield quality of mandarin (Citrus reticulata Blanco.). Res. J. Agril. Biol. Sci., 5:127-137

  • Nemec, S., Datnoff, L.E. and Strandbery, T. 1996. Efficacy of biocontrol agents in planting mixes to colonize plant ischolar_main and control ischolar_main diseases of vegetables and citrus. Crop Protect., 15:735743

  • Nelson, L.M. 2004. Plant growth promoting rhizobacteria (PGPR): prospects for new inoculants. Crop Mgt., doi:10.1094/CM-2004-0301-05-RV

  • Orhan, E., Esitken, A., Ercisli, S., Turan, M. and Sahin, F. 2006. Effects of Plant Growth Promoting Rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Sci. Hortic., 111:38-43

  • Pan, S.Q., Ye, X.S. and Kue, J. 1991. A technique for detection of chitinase, β-1,3- glucanase and protein patterns after a single separation using polyacrylamide gel electrophoresis and isoelectric focusing. Phytopathol., 81:970-974

  • Pikovskaya, R.I. 1948. Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiologia, 17:362-370

  • Ramarathnam, R., Dilantha, F.W.G. and de Kievit, T. 2011. The role of antibiosis and induced systemic resistance, mediated by strains of Pseudomonas chlororaphis, Bacillus cereus and B. amyloliquefaciens, in controlling blackleg disease of canola. BioControl, 56:225-235

  • Schwyn, B. and Neiland, J.B. 1987. Universal chemical assay for the detection and determination of siderophores. Anal. Biochem., 160:47-56

  • Stafford, W.H.L., Baker, G.C. and Brown, S.A. 2005. Environ. Microbiol., 7:1755-1768

  • Woitke, M., Junge, H. and Schnitzler, W.H. 2004. Bacillus subtilis as growth promotor in hydroponically grown tomatoes under saline conditions. Acta Hort., 659:363-369

  • Yanni, Y.G., Rizk, R.Y., Corich, V., Squartini, A., Ninke, K., Philip-Hollingsworth, S., Orgambide, G., de Bruijn, F., Stoltzfus, J., Buckley, D., Schmidt, T.M., Mateos, P.F., Ladha, J.K. and Dazzo, F.B. 1997. Natural endophytic association between Rhizobium leguminosarum bv. trifolii and rice ischolar_mains and assessment of its potential to promote rice growth. Pl. Soil, 194:99-114

  • Yildirim, E., Donmez, M.F. and Turan, M. 2008a. Use of bioinoculants in ameliorative effects on radish plants under salinity stress. J. Pl. Nutr., 31:2059-2074

  • Yildirim, E., Turan, M. and Donmez, M.F. 2008b. Mitigation of salt stress in radish (Raphanus sativus L.) by plant growth promoting rhizobacteria. Romanian Biotechnol. Lett., 13:3933-3943

  • Yuan, J., Raza, W., Shen, Q. and Huang, Q. 2012. Antifungal activity of Bacillus amyloliquefaciens NJN-6 volatile compounds against Fusarium oxysporum f. sp. cubense. Appl. Environ. Microbiol., 78:5942-5944

  • Ziedan, E.H.E. and Eman, S.H. 2002. Biological control of ischolar_main rot disease on mandarin by antagonistic strain of Bacillus megatherium. Anna. Agril. Sci., 47:1021-1031


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  • PGPR in Managing Root Rot Disease and Enhancing Growth in Mandarin (Citrus reticulata Blanco.) Seedlings

Abstract Views: 374  |  PDF Views: 164

Authors

B. N. Chakraborty
Immuno-Phytopathology Laboratory, Department of Botany, University of North Bengal, Siliguri, Darjeeling, West Bengal - 734 013, India
S. Allay
Immuno-Phytopathology Laboratory, Department of Botany, University of North Bengal, Siliguri, Darjeeling, West Bengal - 734 013, India
A. P. Chakraborty
Immuno-Phytopathology Laboratory, Department of Botany, University of North Bengal, Siliguri, Darjeeling, West Bengal - 734 013, India
U. Chakraborty
Immuno-Phytopathology Laboratory, Department of Botany, University of North Bengal, Siliguri, Darjeeling, West Bengal - 734 013, India

Abstract


Decline in general plant-health and fruit production in mandarin influenced by abiotic and biotic factors is a major threat to cultivars grown in Darjeeling and Sikkim hills. Fusarium ischolar_main rot, caused by F. oxysporum, is one of the most serious diseases afflicted during early plant growth stage in Citrus. To address this, seven PGPR isolates - Pseudomonas poae (RMK03), Bacillus stratosphericus (RHS/CL-01), Ochrobactrum anthropi, Paenibacillus lentimorbus, Bacillus pumilus, Bacillus megaterium and Bacillus amyloliquefaciens were isolated from the rhizosphere of Citrus reticulata, C. limonia and Camellia sinensis, and used for evaluating their effect on growth of mandarin seedlings. Pseudomonas poae showed in vitro antagonism to Fusarium oxysporum. Better growth enhancement was noticed with P. poae, B. stratosphericus, O. anthropi and B. pumilus. Enhanced activity of chlorophyll, total protein, phenol, four major defense enzymeschitinase, β-1, 3-glucanase, peroxidase and phenyalanine ammonia lyase was observed upon application of PGPR. P. poae also suppressed ischolar_main rot caused by Fusarium oxysporum. Use of PGPR, which promote growth besides reducing disease severity to some extent, may lead to use of eco-friendly approaches for controlling plant diseases.

Keywords


PGPR, Mandarin, Root Rot, Citrus, Bacterial Isolates.

References