Refine your search
Collections
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Virupakshaiah, D. B. M.
- Significance of altered glycosyltransferase expression levels in oral cancer
Abstract Views :171 |
PDF Views:99
Authors
Mallikarjun Goni
1,
Anil Kumar Desai
2,
Niranjan Kumar
2,
Veda Hegde
3,
Palaksha Kanive Javaregowda
4,
D. B. M. Virupakshaiah
5
Affiliations
1 SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, Department of Biotechnology, Basaveshwar Engineering College, Vidyagiri, Bagalkot 587 102, IN
2 SDM Craniofacial Surgery and Research Centre, A Constituent unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, IN
3 Department of Oral Pathology, SDM College of Dental Sciences and Hospital, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, IN
4 SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, IN
5 Department of Microbiology, Davangere University, Shivagangotri, Davangere 577 002, IN
1 SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, Department of Biotechnology, Basaveshwar Engineering College, Vidyagiri, Bagalkot 587 102, IN
2 SDM Craniofacial Surgery and Research Centre, A Constituent unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, IN
3 Department of Oral Pathology, SDM College of Dental Sciences and Hospital, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, IN
4 SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, IN
5 Department of Microbiology, Davangere University, Shivagangotri, Davangere 577 002, IN
Source
Current Science, Vol 123, No 1 (2022), Pagination: 52-58Abstract
We evaluate the relative quantification of mRNA expression levels of the gene encoding an enzyme that catalyses initial step in glycosylation of dolichol-linked oligosaccharide such as dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (DPAGT1). Furthermore, beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (MGAT3) and alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase (MGAT5) mediated N-glycosylation, selected a2, 3, a2, 6 sialyltransferases families and sialidase by using the Real-Time Quantitative Reverse Transcription PCR (qRT-PCR). Thirty human oral cancer tissue samples and twelve control samples were examined. Results indicated significant upregulation of ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 (ST6GALNAC1) in oral cancer compared to controls. Additionally, the expressions of DPAGT1, MGAT3, MGAT5, ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3GAL1), ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1), and ST6GALNAC1 were considerably higher in the tumour in tongue lesion compared to the buccal cavity. Hence, our experiment demonstrates significance of glycosyltransferases in the progression of oral cancerKeywords
Aberrant Glycosylation, Invasion, Metastasis, Oral Cancer, SialyltransferaseFull Text
References
- Jamal, B. et al., Aberrant amplification of the crosstalk between canonical Wnt signaling and N-glycosylation gene DPAGT1 promotes oral cancer. Oral Oncol., 2012, 48(6), 523–529.
- Liu, G. et al., N-glycosylation induces the CTHRC1 protein and drives oral cancer cell migration. J. Biol. Chem., 2013, 288(28), 20217–20227.
- Liao, C., An, J., Tan, Z., Xu, F., Liu, J. and Wang, Q., Changes in protein glycosylation in head and neck squamous cell carcinoma. J. Cancer, 2021, 12(5), 1455–1466.
- Guu, S. Y. et al., Serum N-glycome characterization and anticarbohydrate antibody profiling in oral squamous cell carcinoma patients. PLoS ONE, 2017, 12(6), e0178927.
- Negi, M., Bansal, S., Puri, A. and Nangia, R., Tumor markers in oral squamous cell carcinoma as an adjunct to diagnosis: an insight. Indian J. Dent. Sci., 2018, 10, 190.
- Büll, C., Stoel, M. A., Den Brok, M. H. and Adema, G. J., Sialic acids sweeten a tumor’s life. Cancer Res., 2014, 74(12), 3199– 3204.
- Goni, M., Javaregowda, P. K., Chachadi, V. and DBM, V., Sialic acids: an avenue to target cancer progression, metastasis and resistance to therapy. Forum Clin. Oncol., 2021, 12(2), 1–9.
- Varelas, X., Bouchie, M. P. and Kukuruzinska, M. A., Protein Nglycosylation in oral cancer: dysregulated cellular networks among DPAGT1, E-cadherin adhesion and canonical Wnt signaling. Glycobiology, 2014, 24(7), 579–591.
- Nagae, M. et al., Structure and mechanism of cancer-associated Nacetylglucosaminyltransferase-V. Nature Commun., 2018, 9(1), 3380.
- Goni, M., Hegde, V., Kumar, N., Desai, A. K., Javaregowda, P. K. and DBM, V., Evaluation of oral cancer associated glycosyltransferase expression levels in peripheral blood mononucleated cells. Adv. Cancer Biol. – Metastasis, 2021, 3, 100013.
- Pinho, S. S. et al., The role of N-acetylglucosaminyltransferase III and V in the post-transcriptional modifications of E-cadherin Hum. Mol. Genet., 2009, 18(14), 2599–2608.
- Pinho, S. S. et al., Loss and recovery of Mgat3 and GnT-III mediated E-cadherin N-glycosylation is a mechanism involved in epithelial–mesenchymal–epithelial transitions. PLoS ONE, 2012, 7(3), e33191.
- Pietrobono, S. and Stecca, B., Aberrant sialylation in cancer: biomarker and potential target for therapeutic intervention? Cancers (Basel), 2021, 13(9), 2014.
- Mehta, K. A., Patel, K. A., Pandya, S. J. and Patel, P. S., Aberrant sialylation plays a significant role in oral squamous cell carcinoma progression. J. Oral. Pathol. Med., 2020, 49(3), 253–259.
- Munkley, J. and Scott, E., Targeting aberrant sialylation to treat cancer. Medicines, 2019, 6(4), 102.
- Livak, K. J. and Schmittgen, T. D., Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆CT method. Methods, 2001, 25(4), 402–408.
- Vajaria, B. N. and Patel, P. S., Molecular events in tobacco related oral cavity cancers. Oral Cancer, Chapter 2, 2016; www.avidscience.com.
- Johnson, D. E. et al., Head and neck squamous cell carcinoma. Nature Rev. Dis. Prim., 2020, 6(1), 92.
- Vajaria, B. N. et al., Our studies on aberrant glycosylation in oral cancer and insights on glycosylation as a hallmark of cancer. Int. J. Res. Rev., 2019, 6(11), 487–495.
- Ogawa, T. et al., ST6GALNAC1 plays important roles in enhancing cancer stem phenotypes of colorectal cancer via the Akt pathway. Oncotarget, 2017, 8(68), 112550–112564.
- Munkley, J. and Elliott, D. J., Sugars and cell adhesion: the role of ST6GalNAc1 in prostate cancer progression. Cancer Cell Microenviron., 2016, 3(1), e1174.
- Ozaki, H. et al., Enhancement of metastatic ability by ectopic expression of ST6GalNAcI on a gastric cancer cell line in a mouse model. Clin. Exp. Metastasis, 2012, 29(3), 229–238.
- Gaddikeri, S., Bhrany, A. and Anzai, Y., Perineural invasion of skin cancers in the head and neck: an uncommon phenomenon revisited. Otolaryngology, 2014, 4(3), 169.
- Marchesi, F., Piemonti, L., Mantovani, A. and Allavena, P., Molecular mechanisms of perineural invasion, a forgotten pathway of dissemination and metastasis. Cytokine Growth Factor Rev., 2010, 21(1), 77–82