Current Science

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The definitive diagnosis ability of IgM ELISA, nested RT-PCR and real-time quantitative PCR (qPCR) was evaluated for Chikungunya diagnosis using 180 clinical samples. Real-time qPCR showed a higher sensitivity (88.3%) for Chikungunya diagnosis in the early stages of infection, while IgM ELISA proved sensitive for the late stages of illness (81.8%). The results suggest that the application of both IgM ELISA and RTPCR based assays will be ideal for definitive diagnosis of Chikungunya during outbreaks.

Keywords

Chikungunya, IgM Capture ELISA, Nested RT-PCR, qPCR.
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