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Here we compare the in-house ELISA technique for estimation of neuron-specific enolase (NSE) and S-100ββ (glial-specific protein) peptides with a commercial kit in serum samples of acute ischaemic stroke (AIS) patients. Nineteen improved and four expired AIS patients were included for the study. NSE concentrations were significantly higher (P < 0.05) in expired as compared with improved AIS patients by both inhouse ELISA and commercial kit method. Estimation of S-100ββ by only in-house ELISA showed significantly high (P < 0.05) levels in expired AIS patients. Peptide-based estimation of NSE and S-100ββ may be used for prognosis of AIS patients.


Acute Ischaemic Stroke, Glial-Specific Protein, Neurological Disease, Neuron-specific Enolase, Prognosis.
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