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Frequency-Dependent Response of Chromobacterium violaceum to Sonic Stimulation and Altered Gene Expression Associated with Enhanced Violacein Production at 300 Hz


Affiliations
1 Institute of Science, Nirma University, Ahmedabad 382 481, India
 

In this study, Chromobacterium violaceum was subjected to sonic (100–2000 Hz) stimulation. Sound waves of 300 Hz frequency promoted bulk production of the quorum-regulated pigment, violacein. Whole transcriptome analysis indicated that a total of 342 genes (i.e. 4.63% of the whole genome) were significantly upregulated in the sonic stimulated culture. Enhanced violacein production in the sound-stimulated culture seems to have stemmed from enhanced expression of the genes involved in pentose phosphate pathway, resulting in an increased availability of erythrose-4-phosphate to be used in the synthesis of tryptophan – the precursor of violacein synthesis. This study is a good demonstration of the ability of sound waves to alter bacterial metabolism.

Keywords

Altered Gene Expression, Chromobacterium violaceum, Sonic Stimulation, Violacein.
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  • Frequency-Dependent Response of Chromobacterium violaceum to Sonic Stimulation and Altered Gene Expression Associated with Enhanced Violacein Production at 300 Hz

Abstract Views: 273  |  PDF Views: 74

Authors

Chinmayi Joshi
Institute of Science, Nirma University, Ahmedabad 382 481, India
Pooja Patel
Institute of Science, Nirma University, Ahmedabad 382 481, India
Abhishek Singh
Institute of Science, Nirma University, Ahmedabad 382 481, India
Jinal Sukhadiya
Institute of Science, Nirma University, Ahmedabad 382 481, India
Vidhi Shah
Institute of Science, Nirma University, Ahmedabad 382 481, India
Vijay Kothari
Institute of Science, Nirma University, Ahmedabad 382 481, India

Abstract


In this study, Chromobacterium violaceum was subjected to sonic (100–2000 Hz) stimulation. Sound waves of 300 Hz frequency promoted bulk production of the quorum-regulated pigment, violacein. Whole transcriptome analysis indicated that a total of 342 genes (i.e. 4.63% of the whole genome) were significantly upregulated in the sonic stimulated culture. Enhanced violacein production in the sound-stimulated culture seems to have stemmed from enhanced expression of the genes involved in pentose phosphate pathway, resulting in an increased availability of erythrose-4-phosphate to be used in the synthesis of tryptophan – the precursor of violacein synthesis. This study is a good demonstration of the ability of sound waves to alter bacterial metabolism.

Keywords


Altered Gene Expression, Chromobacterium violaceum, Sonic Stimulation, Violacein.

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DOI: https://doi.org/10.18520/cs%2Fv115%2Fi1%2F83-90