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It is now well documented that arbuscular mycorrhizal fungi (AMF) improve fitness and growth of plants which are important in agriculture, horticulture and forestry. An important task in most AM studies is to determine accurately the number of infective propagules (IP) of AMF in soil, substrate, or inoculum. The most probable number (MPN) technique (or method of ultimate dilution) for enumerating viable microorganisms, is a possible solution to the problems faced when using the usual methods of counting AMF endophyte propagules.
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