Current Science

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Clostridium difficile with its virulence factors A and B toxins cause Pseudomembranous colitis. Bacterium was isolated from 57 dog and 41 pig diarrheic faecalsamples in cycloserinecefaxitin fructose agar media and molecular detection was done by amplification of gluD gene (755 bp). Variability of toxin genes in positive isolates was tested by multiplex PCR. Detection ofbinary toxin genes (cdtA and cdtB) was also done. Results showed 33.67% positivity with 18 and 15 fromdog and pig respectively, from which 10 and 5 were toxigenic and 11 pig isolates exhibited binary toxin. PCR-RFLP demonstrated toxinotype 0 in all A+B+ isolates.

Keywords

Diarrhoea, NE India, Pseudomembranous Colitis, Toxin-Typing, Virulence.
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