An Ontological Design: Two Stage Mouse Skin Carcinogenesis Induced By DMBA and Promoted By Croton Oil

DMBA is a polycyclic aromatic hydrocarbon which may be used as cancer initiator and croton oil is used as cancer promoter in murine skin which composed of phorbol esters. Formation of stable DMBA-DNA adduct can lead to the induction of mutation that activate proto-oncogen or inactivate tumor suppressor genes as an important event during tumor initiation. In mouse skin the promotion process like increased cell proliferation, suppression of apoatosis and induction of the production of ROS and RNS that cause increase membrane lipid peroxidation and decrease cellular antioxidant stores. ROS plays an important role in the process of mutagenesis and carcinogenesis specially tumor promotion. The main aim and rationally of this ontological design is very potent for screening of new cancer chemopreventive agents as well as anticancer drug development.