Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Development and Validation of Derivative Spectrophotometric Method for Simultaneous Estimation of Diclofenac and Pantoprazole in Combined Capsule Dosage form


Affiliations
1 Department of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, India
     

   Subscribe/Renew Journal


Derivative spectroscopy offers a useful approach for the analysis of drugs in multi-component mixtures. In this study a first-derivative spectroscopic method was used for the simultaneous determination of diclofenac and pantoprazole in combined capsule dosage form using the zero-crossing technique. The wavelengths 290.2 nm and 276.2 nm of first derivative spectrum were selected for the estimation of diclofenac and pantoprazole, respectively without mutual interference. The method was linear in the concentration range 15-75μg/ml and 5-25μg/ml for diclofenac and pantoprazole, respectively. Validation studies confirmed the accuracy and precision of the proposed method. The result of the formulation analysis shows that the proposed method can be successfully used for the simultaneous estimation of both the drugs in their combined capsule dosage form.

Keywords

Diclofenac, Pantoprazole, Simultaneous Determination, Derivative Spectrophotometry, Zero Crossing Point
Subscription Login to verify subscription
User
Notifications
Font Size


  • The Merck index, 14th Edn, Published by Merck research laboratories, Division of Merck & Co., INC., Whitehouse station, NJ, USA, 2006, 3081, 7014
  • Tripathi KD, Essentials of Medical Pharmacology, 6th Edn, New Delhi, Jaypee brothers, 2008, 184, 193.
  • RW Lohe, PB Suruse, MK Kale, PR Barethiya, AV Kasture and SW Lohe, “Spectrophotometric Methods for Simultaneous Estimation of Rabeprazole and Diclofenac from Combined Tablet Dosage Form”, Asian Journal of Reseach in Chemistry, 2008, 1(1), 26-28
  • Umarkar AR, Rewatkar NS, Charde MS and Charde RM, “Simultaneous estimation of thiocolchicoside and diclofenac potassium by UV spectrophotometer using multicomponent method”, International Journal of ChemTech Research, 2011, 3(2), 944-947
  • Karthik A, Subramanian G, Kumar AR, Udupaa N, “Simultaneous estimation of paracetamol and domperidone in tablets by reverse phase HPLC method”, Indian Journal of Pharmaceutical Sciences, 2007, 69(1), 142-144
  • Yilmaz B, Asci A, and Palabiyik SS, “HPLC method for determination of diclofenac in human plasma and its application to a Pharmacokinetic study in turkey, Journal of Chromatographic Science, 2011, 49, 422-427
  • Lala LG, D'Mello PM, Naik SR, “HPTLC determination of diclofenac sodium from serum”, Journal of Pharmcy and Biomedical Analysis, 2002, 29(3), 539-544
  • Kumar R, Singh H and Singh P, “Development of UV Spectrophotometric method for estimation of Pantoprazole in pharmaceutical dosage forms”, Journal of Chemical and Pharmaceutical Research, 2011, 3(2), 113- 117
  • Basavaiah K, UR Anil Kumar, “Sensitive spectrophotometric methods for the determination of pantoprazole sodium in pharmaceutical using bromate-bromide, methyl orange and indigo carmine as reagents”, Indian Journal of Chemical Technology, 2007, 14, 611-615
  • Syed AA, Syeda A., “Neocuproine and Bathocuproine as new reagents for spectrophotometric determination of certain proton pump inhibitors”, Bulletin of the Chemical Society of Ethiopia, 2007, 21(3), 315-321.
  • Kalaichelvi R, Fatima M, Vadivel K, Jayachandran E, “Simple extractive colorimetric determination of pantoprazole sodium by acid-dye complexation method in solid dosage form”, International Journal of Chemistry Research, 2010, 1(1), 6-8
  • Ramakrishna NVS, Vishwottam KN, Wishnu S, Koteshwara M, “High performance liquid chromatography method for the determination of Pantoprazole in human plasma”, Journal of Chromatography B, 2005, 822, 326-329
  • Gosavi SA, Shirkhedkar AA, Jaiswal YS, Surana SJ, “A simple and sensitive HPTLC method for quantitative analysis of pantoprazole sodium sesquihydrate in tablets”, Journal of Planar Chromatography-Modern TLC, 2006, 19, 228-33
  • Patel BH, Suhagia BN, Patel MM, Patel JR, “Simultaneous estimation of pantoprazole and domperidone in pure powder and a pharmaceutical formulation by high perfomance liquid chromatography and highperformance thin layer chromatography methods”, Journal of AOAC International, 2007, 90(1), 142-146
  • International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human use, Validation of Analytical Procedures: Text and Methodology ICH Q2(R1), 2005

Abstract Views: 436

PDF Views: 2




  • Development and Validation of Derivative Spectrophotometric Method for Simultaneous Estimation of Diclofenac and Pantoprazole in Combined Capsule Dosage form

Abstract Views: 436  |  PDF Views: 2

Authors

Khushbu Naik
Department of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, India
Gunjansinh Parmar
Department of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, India
Jalpesh Ahiya
Department of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, India
Kunjan Bodiwala
Department of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, India
Shailesh Shah
Department of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, India

Abstract


Derivative spectroscopy offers a useful approach for the analysis of drugs in multi-component mixtures. In this study a first-derivative spectroscopic method was used for the simultaneous determination of diclofenac and pantoprazole in combined capsule dosage form using the zero-crossing technique. The wavelengths 290.2 nm and 276.2 nm of first derivative spectrum were selected for the estimation of diclofenac and pantoprazole, respectively without mutual interference. The method was linear in the concentration range 15-75μg/ml and 5-25μg/ml for diclofenac and pantoprazole, respectively. Validation studies confirmed the accuracy and precision of the proposed method. The result of the formulation analysis shows that the proposed method can be successfully used for the simultaneous estimation of both the drugs in their combined capsule dosage form.

Keywords


Diclofenac, Pantoprazole, Simultaneous Determination, Derivative Spectrophotometry, Zero Crossing Point

References