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Development and Validation of Derivative Spectrophotometric Method for Simultaneous Estimation of Levosulpiride and Esomeprazole in Capsule Dosage Form


Affiliations
1 Department of Pharmaceutical Analysis, Maliba Pharmacy College, Bardoli, Gujarat, India
2 Depatment of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, India
     

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The use of first order derivative spectrophotometry allows simultaneous determination of levosulpiride and esomeprazole in fixed dose combination products. The wavelengths 275 nm and 291.5 nm of first derivative spectrum were selected for the estimation of levosulpiride and esomeprazole, respectively without mutual interference. The method was linear in the concentration range 25-125 μg/ml and 5-25 μg/ml for levosulpiride and esomeprazole, respectively. The method was validated in terms of accuracy and precision. The result of the formulation analysis shows that the proposed method can be successfully used for the simultaneous estimation of both the drugs in their combined capsule dosage form.

Keywords

Levosulpiride, Esomeprazole, Ultra-violet spectrophotometry, Derivative spectrophotometry, Zero
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  • Development and Validation of Derivative Spectrophotometric Method for Simultaneous Estimation of Levosulpiride and Esomeprazole in Capsule Dosage Form

Abstract Views: 420  |  PDF Views: 2

Authors

Brijen Vaghela
Department of Pharmaceutical Analysis, Maliba Pharmacy College, Bardoli, Gujarat, India
Gunjansinh Parmar
Depatment of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, India
Shailesh Shah
Depatment of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, India
Dinesh Shah
Depatment of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, India

Abstract


The use of first order derivative spectrophotometry allows simultaneous determination of levosulpiride and esomeprazole in fixed dose combination products. The wavelengths 275 nm and 291.5 nm of first derivative spectrum were selected for the estimation of levosulpiride and esomeprazole, respectively without mutual interference. The method was linear in the concentration range 25-125 μg/ml and 5-25 μg/ml for levosulpiride and esomeprazole, respectively. The method was validated in terms of accuracy and precision. The result of the formulation analysis shows that the proposed method can be successfully used for the simultaneous estimation of both the drugs in their combined capsule dosage form.

Keywords


Levosulpiride, Esomeprazole, Ultra-violet spectrophotometry, Derivative spectrophotometry, Zero

References