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Selection of Suitable Physico-Chemical Conditions for Gluconic Acid Production by a Mutant Aureobasidium Pullulans SG 80
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An experimental study was conducted to improve the production of gluconic acid by the mutant Aureobasidium pullulans SG 80 by optimizing different physic-chemical conditions for fermentation. After optimization of different factors, maximum production was obtained with : glucose,8%; (NH4)2SO4,1.0%; K2HPO4,0.15%; CaCO3, 0.25% , MnSO4,,15%; and MgSO4.7H2O,0.25% with maximum utilization of sugar at pH,6.5;volume of medium,30ml; cell density, 5x106 cells/ml; shaker speed ,200 rpm and temperature, 300C.
Keywords
Gluconic Acid, Aureobasidium Pullulans, Fermentation, Optimization,utilization
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