Asian Journal of Research in Chemistry

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Reverse Phase High Performance Liquid Chromatographic Method for the Simultaneous Estimation of Amoxicillin Trihydrate and Tinidazole in the Tablet Dosage Form


Affiliations
1 Department of Pharmaceutical Analysis and Quality assurance, Lalitha College of Pharmacy, Venkatapur (V), Ghatkesar (M), R.R Dist., A.P.-501301
     

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A simple, rapid and selective RP -HPLC method has been developed for determination of Amoxicillin trihydrate and Tinidazole in the pharmaceutical dosage form. The chromatographic separation was achieved with reverse phase phenomenex ® (Luna 5μ C18(2) 100A (250 × 4.60 mm) Column in isocratic mode and mobile phase containing 25mM Potassium dihydrogen phosphate (adjusted to pH 4.5 by Ortho-phosphoric acid) and Acetonitrile in the ratio of 40:60 % v/v. The flow rate was 1mL min-1 and effluent was monitored at 238 nm. Retention time was found to be 2.053 min for Amoxicillin trihydrate and 2.944 min for Tinidazole , respectively. Linear regression analysis data for the calibration plot showed that there was good linear relationship between response and concentration in the range of 5-50 μg/ml for both Amoxicillin trihydrate and Tinidazole , respectively. Percentage recoveries obtained for Amoxicillin trihydrate and Tinidazole were 100.87% and 99.52% respectively. The proposed method is precise, accurate, selective and rapid for the simultaneous determination of Amoxicillin trihydrate and Tinidazole in pharmaceutical dosage forms.

Keywords

Amoxicillin Trihydrate, Tinidazole , RP-HPLC Method, Validation, Simultaneous Estimation
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  • Subhash Chandra Bose, Kotte., Vijaya Kumar. Tulam, Ramakoteswara Rao. Chinta, S. Shriram Raghavan, P.K. Dubey and P.M. Murali, 2012. Qualitative Analysis of, Ampicillin, Cephalexin By Quadrupole –Time of Flight (LCMS) using Electrospray Ionization. International Journal of Chem Tech Research, 4: 855-861.

  • Rahman S., A. Ahuja, J. Ali and K. Khar, 2004. Simultaneous spectrophotometric determination of amoxycillin trihydrate and metronidazole in dental films. Indian J Pharm Sci, 66: 135-36.

  • Suresh Babu G., Manzoor Ahmed and A. Sathish Kumar Shetty, 2011. Development and Validation of Amoxicillin by RP-HPLC Method in Bulk drug and Pharmaceutical dosage forms. International Journal of Chem Tech Research, 3:1037-1041.

  • Indian Pharmacopoeia, 2003. Ghaziabad, The Indian pharmacopoeia commission.3: 2090

  • British Pharmacopoeia 2003, Her Majesty’s stationary office. London, 3 :719-21.

  • http://www.drugbank.ca/drugs/DB01060.

  • Khaja Pasha., Asgar Ali, Shahana Bana and Syeda Humair, 2010. Reverse phase – HPLC method for the analysis of tinidazole in pharmaceutical dosage form & bulk drug. International Journal of Pharmacy and Pharmaceutical Sciences, 2: 2

  • Sobel J.D., P. Nyirjesy and W. Brown, 2001. Tinidazole therapy for metronidazole- resistant trichomoniasis, Clin. Infect. Dis., 33: 1341–6.

  • David R. Hill. and Timothy B. Gardner, 2001. Treatment of Giardiasis. Clin Microbiol Rev. 2001 January; 14: 14–128.

  • British Pharmacopoeia Vol. III, United Kingdom: The Stationary office on the behalf of MHRA, 2009, 2037.

  • http://www.drugbank.ca/drugs/DB00911

  • Bobrowska G.E., 2001. Determination of amoxicillin and clavulanic acid in some pharmaceutical preparations by derivative spectrophotometry. Mikrochim Acta, 136: 31-34.

  • Mascher J.H. and C. Kikuta, 1998. Determination of Amoxicillin in Human Serum and Plasma by High Performance Liquid Chromatography and Online Post Column Derivatisation. J Chromagr A, 812: 221-26.

  • Abreu L.R. and R.A. Ortiz, 2003. HPLC determination of amoxicillin comparative bioavailability in healthy volunteers after a single dose administration. J Pharm Sci, 6: 223-230.

  • Yuan Z., H.Q. Russlie and D.M. Canafax, 1997. High Performance Liquid Chromatographic Analysis of Amoxycillin in Human and Chinchilla Plasma, Middle Ear Fluid and Whole Blood. J Chromatogr B, 692:361-66.

  • Zarapkar S.S. and S.H. Rane, 2000. Reverse phase high performance liquid chromatographic determination of amoxicillin and ambroxol hydrochloride in tablets. Indian Drugs, 37:246-250.

  • Zarapkar S.S., S.S. Kolte and N.P. Bhandari, 1998. High performance liquid chromatographic determination of amoxicillin trihydrate and probenicid simultaneously from pharmaceutical preparation. Indian Drugs, 35: 107-109.

  • Wibawa J.I.D., D. Fowkes, P.N. Shaw and D.A. Barrett, 2002. Measurement Of Amoxicillin In Plasma And Gastric Samples Using High Performance Liquid Chromatography With Flurimetric Detection J Chromatogr B, 774:141-148.

  • Menelaou A., A.A. Soxmogyi, M.L. Barclay and F. Bochner, 1999. Simultaneous quantification of amoxicillin and metrinidazole in plasma using high performance liquid chromatography with photodiode array detection. J Chromatogr B Appl, 731:261-266.

  • Qureshi S.Z., T. Qayoom and M.I. Helalet, 1999. Simultaneous spectrophotometric and voltametric determinations of amoxicillin, ampicillin and cloxacillin in drug formulations: reaction mechanism in the base catalyzed hydrolysis followed by oxidation with iodate in dilute acid solution. J Pharm Biomed Anal, 21: 473-482.

  • Panzade P.D. and K.R. Mahadik, 2001. Simultaneous estimation of ofloxacin and tinidazole in tablet dosage form, Indian Drugs, 38: 368–370.

  • Bombale M.V., S.S. Kadam and S.R. Dhaneshwar, 1997. Estimation of Ciprofloxacin and Tinidazole from a combined dosage form by spectrophotometry, Indian Journal of Pharmaceutical Sciences, 59: 265 – 268.

  • Krishnaiah Y.S.R., S.A. Devi, Y.I. Muzib, R.S. Karthikeyan and V. Satyanarayana, 2002. Estimation of Tinidazole in pharmaceutical dosage forms by reverse phase HPLC, The Antiseptic, 99: 5.

  • Bhatia M.S., S.G. Kaskhedikar, and S.C. Chaturvedi, 1999. High performance liquid chromatographic estimation of ciprofloxacin hydrochloride and tinidazole from tablets. Indian J. Pharm. Sci, 8: 311.

  • Salomies H. and J.P. Salo, 2005. An HPLC study of tinidazole. Chromagraphia, 36: 79.

  • Sorvillo F., L. Smith and P. Kerndt, 2001. Trichomonas vaginalis, HIV and African-Americans. Emerg. Infect. Dis., 7: 927-932.

  • Salo J.P.K. and H. Salomies, 2003. Two stability-indicating UV spetrophotometric methods for the analysis of hydrolyzed tinidazole. J. Pharm. Biomed. Anal., 31: 523-536.

  • Daharwal S.J. and S. Saraf, 2007. Spectrophotometric Determination for the Simultaneous Estimation of Amoxicillin and Tinidazole in Tablet Dosage Form. Indian J Pharm Sci, 41: 35-41.

  • Arindam Basu., Nandini Saha and Inder Singh Rawat, 2011. Simultaneous LC Estimation of Amoxycillin and Tinidazole in Tablet Dosage Form. IJPR, 3:45-48.

  • ICH Harmonized Tripartite Guidelines 1994, code Q2A, Text on validation of Analytical procedure, Step-4, Consensus Guidelines.


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  • Reverse Phase High Performance Liquid Chromatographic Method for the Simultaneous Estimation of Amoxicillin Trihydrate and Tinidazole in the Tablet Dosage Form

Abstract Views: 344  |  PDF Views: 4

Authors

V Bojaraju
Department of Pharmaceutical Analysis and Quality assurance, Lalitha College of Pharmacy, Venkatapur (V), Ghatkesar (M), R.R Dist., A.P.-501301
D Sireesha
Department of Pharmaceutical Analysis and Quality assurance, Lalitha College of Pharmacy, Venkatapur (V), Ghatkesar (M), R.R Dist., A.P.-501301
Mahesh Nasare
Department of Pharmaceutical Analysis and Quality assurance, Lalitha College of Pharmacy, Venkatapur (V), Ghatkesar (M), R.R Dist., A.P.-501301
V.V.L.N. Prasad
Department of Pharmaceutical Analysis and Quality assurance, Lalitha College of Pharmacy, Venkatapur (V), Ghatkesar (M), R.R Dist., A.P.-501301
Prakash V. Diwan
Department of Pharmaceutical Analysis and Quality assurance, Lalitha College of Pharmacy, Venkatapur (V), Ghatkesar (M), R.R Dist., A.P.-501301

Abstract


A simple, rapid and selective RP -HPLC method has been developed for determination of Amoxicillin trihydrate and Tinidazole in the pharmaceutical dosage form. The chromatographic separation was achieved with reverse phase phenomenex ® (Luna 5μ C18(2) 100A (250 × 4.60 mm) Column in isocratic mode and mobile phase containing 25mM Potassium dihydrogen phosphate (adjusted to pH 4.5 by Ortho-phosphoric acid) and Acetonitrile in the ratio of 40:60 % v/v. The flow rate was 1mL min-1 and effluent was monitored at 238 nm. Retention time was found to be 2.053 min for Amoxicillin trihydrate and 2.944 min for Tinidazole , respectively. Linear regression analysis data for the calibration plot showed that there was good linear relationship between response and concentration in the range of 5-50 μg/ml for both Amoxicillin trihydrate and Tinidazole , respectively. Percentage recoveries obtained for Amoxicillin trihydrate and Tinidazole were 100.87% and 99.52% respectively. The proposed method is precise, accurate, selective and rapid for the simultaneous determination of Amoxicillin trihydrate and Tinidazole in pharmaceutical dosage forms.

Keywords


Amoxicillin Trihydrate, Tinidazole , RP-HPLC Method, Validation, Simultaneous Estimation

References