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Evaluation of Antioxidant Capacity by Cyclic Voltammetry of Phoenix dactylifera L. (date palm)


Affiliations
1 Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria., India
2 Department of Exact Sciences, Higher Normal School of Ouargla, 30000 Ouargla, Algeria., India
3 Department of Exact Sciences, Higher Normal School of Ouargla, 30000 Ouargla, Algeria, India
4 Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria, India
5 Department of Agronomy, Faculty of Nature and Life Sciences and Earth Sciences, University of Ghardaia, 47000 Ghardaia, Algeria., India
     

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In this study we used spectrophotometrical techniques to estimate antioxidant efficacy by using 2,2-diphenyl-1- picrylhydrazyl free radical scavenging capacity and reducing power capacity. We have used also electrochemical techniques performed by cyclic voltammetry. The present study examined the antioxidant activities of the methanolic extract (ME) of date palm (Phoenix dactylifera. L) fruits (DPF) of four native cultivars from Algeria, namely, Ghars (Gh), Chtaya (Cht), Degla Beïda (DB) and Tinissine (Tns). Quantitative estimation of total phenol and flavonoid content by a colorimetric assay showed that methanol extracts are rich in these components. The content of total phenolics of different cultivars extracts was in the range of 26.083 - 35.850 GAE/100g DW. while it was the content of total flavonoids of the different cultivars extracts was in the range of 5.276 - 6.055 QE/100g DW. The value of IC50 for DPPH radical test ranged from 0.193 to 0.400mg/ml. All extracts showed very good activity of ferric reducing power, the higher power was in methanolic extract of Tns. The less IC50 value in cyclic voltammetry method (CV), which meet the highest effective antioxidant was 0.121 mg/ml in methanolic extract of Tns.

Keywords

Phoenix dactylifera L, Phenolic content, Flavonoids contents, antioxidant activity, cyclic voltammetry.
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  • F.Z. Bentebba, G. Zineb, and M. Saidi, Phytochemical content and Antioxidant properties of Ghars date palm (Phoenix dactylifera L.) harvested at different stages of maturity. Asian Journal of Research in Chemistry 2020.13(5): 323-326. doi. 10.5958/09744150.2020.00062.0
  • S. Zeroual, I. Daoud, R. Gaouaoui, and S. Ghalem, In vitro and Molecular Docking Studies of DPPH with Phoenix dactylifera L.(Deglet-Nour) Crude Fruits extracts and Evaluation of their Antioxidant Activity. Asian Journal of Research in Chemistry 2020.13(1): 52-59. doi. 10.5958/0974-4150.2020.00012.7
  • M. Abdeldjabbar, D. Messaouda, R. Zhour, and B. Cheyma, Comparative Analysis of Total Phenolics, Flavonoid content and Antioxidant profile of date palm (Phoenix dactylifera L.) with different watering water from Oued Soufin Algeria. Asian Journal of Research in Chemistry 2020.13(1): 28-32. doi. 10.5958/09744150.2020.00007.3
  • S.K. Sripathi and P. Lalitha, Electrochemical Behavior of the Aqueous Fraction of the Ethanol Extract of Pisonia grandis (R. Br) at Glassy Carbon Electrode. Asian Journal of Research in Chemistry 2011.4(5): 775-778. doi. 10.5958/0974-4150
  • á.E. Jose and á.P. Selvam, Identification of Phytochemical Constituents in the Leaf Extracts of Azimaátetracantha Lam using Gas Chromatography-Mass Spectrometry (GC-MS) analysis and Antioxidant Activity. Asian Journal of Research in Chemistry 2018.11(6): 857-862. doi.10.5958/0974-4150.2018.00150.5
  • P. Shlini and K. Murthy, Extraction of phenolics, proteins and antioxidant activity from defatted tamarind kernel powder. Asian Journal of Research in Chemistry 2011.4(6): 936-941. doi.10.5958/0974-4150
  • N.A. AlFaris, J.Z. AlTamimi, F.A. AlGhamidi, N.A. Albaridi, R.A. Alzaheb, D.H. Aljabryn, A.H. Aljahani, and L.A. AlMousa, Total phenolic content in ripe date fruits (Phoenix dactylifera L.): A systematic review and meta-analysis. Saudi Journal of Biological Sciences 2021 doi.org/10.1016/j.sjbs.2021.03.033
  • M.Z. Alam, M.S. Alhebsi, S. Ghnimi, and A. Kamal-Eldin, Inability of total antioxidant activity assays to accurately assess the phenolic compounds of date palm fruit (Phoenix dactylifera L.).NFS Journal 2021.22: 32-40. doi.org/10.1016/j.nfs.2021.01.001
  • M.J. Amiot, A. Fleuriet, and J.J. Macheix, Importance and evolution of phenolic compounds in olive during growth and maturation. Journal of Agricultural and Food Chemistry 1986.34(5): 823-826. doi.org/10.1021/jf00071a014
  • C. Bensaci, Z. Ghiaba, M. Dakmouche, M. Saidi, M. Hadjadj, A. Belfar, and M. Belguidoum, Studies on Antibacterial Compounds from Methanolic Extracts of Date Palm (Phoenix dactylifera L.) fruits from Algeria. Asian Journal of Research in Chemistry 2019.12(6): 338-340. doi. 10.5958/0974-4150.2019.00063.4
  • M. Al-Farsi, C. Alasalvar, A. Morris, M. Baron, and F. Shahidi, Comparison of antioxidant activity, anthocyanins, carotenoids, and phenolics of three native fresh and sun-dried date (Phoenix dactylifera L.) varieties grown in Oman. J Agric Food Chem 2005.53(19): 7592-9. doi.org/10.1021/jf050579q
  • D.-O. Kim, S.W. Jeong, and C.Y. Lee, Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. FoodChemistry 2003.81(3): 321-326. doi.org/10.1016/S03088146(02)00423-5
  • A. Kumaran and R. Joel Karunakaran, In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT - Food Science and Technology 2007.40(2): 344-352. doi.org/10.1016/j.lwt.2005.09.011
  • R.S. Govardhan Singh, P.S. Negi, and C. Radha, Phenolic composition, antioxidant and antimicrobial activities of free and bound phenolic extracts of Moringa oleifera seed flour. Journal of Functional Foods 2013.5(4): 1883-1891. doi.org/10.1016/j.jff.2013.09.009
  • Z. Ghiaba, M. Yousfi, M. Hadjadj, and M. Saidi, Study of antioxidant properties of five Algerian date (phoenix dactylifera L) cultivars by cyclic voltammetric technique. 2014
  • N.G. Etsassala, T. Waryo, O.K. Popoola, A.O. Adeloye, E.I.Iwuoha, and A.A. Hussein, Electrochemical screening and evaluation of lamiaceae plant species from South Africa with potential tyrosinase activity. Sensors 2019.19(5): 1035.doi.org/10.3390/s19051035
  • C. Le Bourvellec, D. Hauchard, A. Darchen, J.-L. Burgot, and M.L. Abasq, Validation of a new method using the reactivity of electrogenerated superoxide radical in the antioxidant capacity determination of flavonoids. Talanta 2008.75(4): 1098-1103. doi.10.1016/j.talanta.2008.01.007
  • A. Mansouri, G. Embarek, E. Kokkalou, and P. Kefalas, Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chemistry 2005.89(3): 411-420.doi.org/10.1016/j.foodchem.2004.02.051
  • C. Alem, J. Ennassir, M. Benlyas, A.N. Mbark, and Y.F. Zegzouti, Phytochemical compositions and antioxidant capacity of three date (Phoenix dactylifera L.) seeds varieties grown in the South East Morocco. Journal of the Saudi Society of Agricultural Sciences 2017.16(4): 350-357. doi.org/10.1016/j.jssas.2015.11.002
  • S. Al Harthi, A. Mavazhe, H. Al Mahroqi, and S.A. Khan, Quantification of phenolic compounds, evaluation of physicochemical properties and antioxidant activity of four date (Phoenix dactylifera L.) varieties of Oman. Journal of Taibah University Medical Sciences 2015.10(3): 346-352. doi.org/10.1016/j.jtumed.2014.12.006
  • F. Mihoub, F. Gourchala, and S. Toumi, Bioactivity of Algerian palm dates Phoenix dactylifera L. Ukrainian food journal 2019(8, Issue 2): 249-259. doi. 10.24263/2304- 974X-2019-8-2-5 22.
  • E.A. Saleh, M.S. Tawfik, and H.M. Abu-Tarboush, Phenolic contents and antioxidant activity of various date palm (Phoenix dactylifera L.) fruits from Saudi Arabia. Food and Nutrition Sciences 2011.2011 doi. 10.4236/fns.2011.210152
  • Z. Benmeddour, E. Mehinagic, D. Le Meurlay, and H. Louaileche, Phenolic composition and antioxidant capacities of ten Algerian date (Phoenix dactylifera L.) cultivars: a comparative study. Journal of Functional Foods 2013.5(1): 346-354. doi.org/10.1016/j.jff.2012.11.005
  • I. Hamad, H. AbdElgawad, S. Al Jaouni, G. Zinta, H. Asard, S.Hassan, M. Hegab, N. Hagagy, and S. Selim, Metabolic analysis of various date palm fruit (Phoenix dactylifera L.) cultivars from Saudi Arabia to assess their nutritional quality. Molecules 2015.20(8): 13620-13641. doi.org/10.3390/molecules200813620
  • M. Al-Mamary, M. Al-Habori, and A.S. Al-Zubairi, The in vitro antioxidant activity of different types of palm dates (Phoenix dactylifera) syrups. Arabian Journal of Chemistry 2014.7(6): 964971. doi.org/10.1016/j.arabjc.2010.11.014
  • M. Singhal, A. Paul, and H.P. Singh, Synthesis and reducing power assay of methyl semicarbazone derivatives. Journal of Saudi Chemical Society 2014.18(2): 121-127.doi.org/10.1016/j.jscs.2011.06.004
  • G. Zineb, M. Boukouada, A. Djeridane, M. Saidi, and M. Yousfi, Screening of antioxidant activity and phenolic compounds of various date palm (Phoenix dactylifera) fruits from Algeria. Mediterranean Journal of Nutrition and Metabolism 2012.5(2): 119-126. doi.10.1007/s12349-011-0082-7
  • J. Elias, M. Rajesh, N. Anish, P. Deepa, and N. Jayan, In vitro antioxidant activity of the methanolic extract of Simaruba glaucaDC. Asian Journal of Research in Chemistry 2010.3(2): 312-315.
  • F. Guemari, S.E. Laouini, A. Rebiai, and A. Bouafia, Phytochemical screening and Identification of Polyphenols, Evaluation of Antioxidant activity and study of Biological properties of extract Silybum marianum (L.). Asian Journal of Research in Chemistry 2020.13(3): 190-197. doi. 10.5958/09744150.2020.00037.1
  • S. Hachani, C. Hamia, S. Boukhalkhal, A.M. Silva, A. Djeridane, and M. Yousfi, Morphological, physico-chemical characteristics and effects of extraction solvents on UHPLC-DAD-ESI-MSn profiling of phenolic contents and antioxidant activities of five date cultivars (Phoenix dactylifera L.) growing in Algeria. NFS
  • journal 2018.13: 10-22. doi.org/10.1016/j.nfs.2018.10.001
  • B. Sultana, B. Fatima, and M. Mushtaq, In vitro synergism of antimutagenic and antioxidant activities of Phoenix dactylifera fruit. Food Science and Biotechnology 2014.23(3): 881-887. doi. 10.1007/s10068-014-0118-0
  • S. Malik and A. Choudhary, Anti-Oxidant Activity of Novel 5- Substituted Arylidene-3-SubstitutedBenzyl-Thiazolidine-2, 4-Diones. Asian Journal of Research in Chemistry 2011.4(1): 120-122.
  • S. Amamra, M.E. Cartea, O.E. Belhaddad, P. Soengas, A.Baghiani, I. Kaabi, and L. Arrar, Determination of total phenolics contents, antioxidant capacity of Thymus vulgaris extracts using electrochemical and spectrophotometric methods. Int. J. Electrochem. Sci 2018.13(8): 7882-7893 . doi:10.20964/2018.08.57
  • C. Bensaci, Z. Ghiaba, M. Dakmouche, A. Belfar, M. Belguidoum, F.Z. Bentebba, M. Saidi, and M. Hadjadj, In Vitro Evaluation of Antioxidant Potential of Date Palm Collected in Algeria using
  • Electrochemical and Spectrophotometrical Techniques. Korean Chemical Engineering Research 2021.59(2): 153-158. doi. 10.9713/kcer.2021.59.2.153

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  • Evaluation of Antioxidant Capacity by Cyclic Voltammetry of Phoenix dactylifera L. (date palm)

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Authors

Assia Belfar
Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria., India
Assia Belfar
Department of Exact Sciences, Higher Normal School of Ouargla, 30000 Ouargla, Algeria., India
Cheyma Bensaci
Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria., India
Cheyma Bensaci
Department of Exact Sciences, Higher Normal School of Ouargla, 30000 Ouargla, Algeria, India
Mahdi Belguidoum
Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria, India
Mahdi Belguidoum
Department of Agronomy, Faculty of Nature and Life Sciences and Earth Sciences, University of Ghardaia, 47000 Ghardaia, Algeria., India
Mokhtar Saidi
Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria., India
Zineb Ghiaba
Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria., India

Abstract


In this study we used spectrophotometrical techniques to estimate antioxidant efficacy by using 2,2-diphenyl-1- picrylhydrazyl free radical scavenging capacity and reducing power capacity. We have used also electrochemical techniques performed by cyclic voltammetry. The present study examined the antioxidant activities of the methanolic extract (ME) of date palm (Phoenix dactylifera. L) fruits (DPF) of four native cultivars from Algeria, namely, Ghars (Gh), Chtaya (Cht), Degla Beïda (DB) and Tinissine (Tns). Quantitative estimation of total phenol and flavonoid content by a colorimetric assay showed that methanol extracts are rich in these components. The content of total phenolics of different cultivars extracts was in the range of 26.083 - 35.850 GAE/100g DW. while it was the content of total flavonoids of the different cultivars extracts was in the range of 5.276 - 6.055 QE/100g DW. The value of IC50 for DPPH radical test ranged from 0.193 to 0.400mg/ml. All extracts showed very good activity of ferric reducing power, the higher power was in methanolic extract of Tns. The less IC50 value in cyclic voltammetry method (CV), which meet the highest effective antioxidant was 0.121 mg/ml in methanolic extract of Tns.

Keywords


Phoenix dactylifera L, Phenolic content, Flavonoids contents, antioxidant activity, cyclic voltammetry.

References