Asian Journal of Research in Chemistry

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Risk assessment of different edible oils subjected to heat at different time intervals and analyzed by titrimetric and instrumental methods and biological estimation on Wistar albino rats


Affiliations
1 Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, India
     

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Oils and fats in the diet serve three functions in the human body: as an energy source, a structural component, and as important biological regulators. Lipid peroxidation may be accelerated by repeatedly heating soy oil. Unsaturated fatty acids that have been oxidised may play a role in the aetiology of a variety of diseases. The purpose of this study was to see if repeatedly heating edible oil had any negative consequences on illness development. In this investigation, four different edible oils were heated to their regular boiling point and then heated for another 3 hours at a 1hour interval. The heated samples were subjected to qualitative examination. After analysing the results, it was discovered that saponification values declined as continuous heating, i.e. 3 hours, was applied to all different oils, resulting in low saponification values when compared to 2 hours, 1 hour, and normal boiling point. Lower the saponification value, the more free fatty acids and triglycerides are produced, which may contribute to cardio vascular illness. The acid values grew as the heating progressed, according to the findings. A high acid number suggests that there is a lot of acid in the system. The values of iodine fell as the heating progressed, according to the results. The presence of saturated fatty acids is indicated by a low iodine value. Saturated fatty acids were detrimental to one's health. As the heating progressed, the peroxide levels grew. Deterioration is indicated by an increase in peroxide value. When compared to other oils, palm oil showed a high saponification value, acid value, peroxide value, and low iodine value. Total cholesterol, tri-glycerides, and glucose levels were higher in 3 hours heated oils than in 2 hours, 1 hour, and at normal boiling point in biological tests .

Keywords

Edible oil, Heating, Fatty acids, Deterioration, Peroxide values.
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  • Bello, “Physico-chemical properties of some commercial groundnut oil products sold in sokoto metropolis, northwest Nigeria,” 2011.

  • V. W. K. Fupi and P. C. Mørk, “Mafura nut oil and meal: processing and purification,” Journal of the American Oil Chemists’ Society, vol. 59, no. 2, pp. 94–98, 1982.

  • H. Khan, M. Fida, I. U. Mohammadzai, and M. Khan, “Estimation of residual nickel and some heavy metals in vanaspati ghee,” Journal of the Chinese Chemical Society, vol. 54, no. 3, pp. 737– 741, 2007.

  • M. Anthea, J. Hopkins, C. W. McLaughlin et al., Human Biology and Health, Prentice Hall, Englewood Cliffs, NJ, USA, 1993.

  • D. Mendil, O. D. Uluozl ¨ u, M. Tuzen, and M. Soylak, “Investiga¨ tion of the levels of some element in edible oil samples produced in Turkey by atomic absorption spectrometry,” Journal of Hazardous Materials, vol. 165, pp. 724–728, 2009.

  • M. Rose, M. Knaggs, L. Owen, and M. Baxter, “A review of analytical methods for lead, cadmium, mercury, arsenic and tin determination used in proficiency testing,” Journal of Analytical Atomic Spectrometry, vol. 16, no. 9, pp. 1101–1106, 2001.

  • E. Johannesen, “Palm fruit oil-much more than ordinary oil,” Journal of Science in Africa Magazine, 2005.

  • R. Manorama and C. Rukmini, “Nutritional evaluation of crude palm oil,” The Oil Technologists’ Association of India, vol. 22, pp.83–87, 1991

  • R. Shaik, Analytical tool for rapid analysis of edible oil [M.S. thesis], Aalborg University Esbjerg, 2008.

  • ISI Hand book of food Analysis (part III) 1984 page 67/ IUPAC2.201 (1979) I.S, 548 (Part-1)-1964 method of sampling and test for oils and fats/ISO 6601 1996 Determination of acid value and acidity.

  • A.O.A.C, Official Method 920.160-Saponfication Number of Oils and Fats/IUPAC 2.202 I.S.I Hand Book of Food Analysis (Part XIII 1984), A.O.A.C, 17th edition, 2000.

  • A.O.A.C, Official Method 920.159-Iodine Absorption Number of Oils and Fats/I.S.I Hand Book of Food Analysis Part-III-1984, A.O.A.C, 17th edition, 2000.

  • A.O.A.C, Official Method 965.33 Peroxide value in oils and fats/Pearson’s composition and analysis of food, 17th edition, pp.641, 2000.

  • F. Camin, R. Larcher, M. Perini et al., “Characterisation of authentic Italian extra-virgin olive oils by stable isotope ratios of C, O and H and mineral composition,” Food Chemistry, vol. 118, no. 4, pp. 901–909, 2010.

  • Z. Mester and R. Sturgeon, Sample Preparation for Trace Element Analysis: Comprehensive Analytical Chemistry, Elsevier Science, Amsterdam, The Netherlands, 2003.

  • E. Weiner, Environmental Chemistry: A Practical Guide for Environmental Professionals, Taylor and Francis Group, Boca Raton, Fla, USA, 2nd edition, 2007.

  • USEPA Method 200.5, “Determination of trace elements in drinking water by axially viewed inductively coupled plasma— atomic emission spectrometry,” Tech. Rep. EPA/600/R-06/115, 2003.

  • O. Ogbonna, L. Jimoh, F. Awagu, and I. Bamishaiye, “Determination of some trace elements in water samples within Kano, Metropolis,” Pelagia Research Library, vol. 2, no. 2, pp. 62– 68, 2011.

  • O. C. Othman and F. N. Ngassapa, “Physico-chemical characteristics of some imported edible vegetable oils and fat marketed in Dar es salaam, Tanzania,” Tanzania Journals of Natural and Applied Science, vol. 1, no. 2, 2010.

  • Manjula B, Shivalinge Gowda KP, Syed Mansoor Ahamed, Nagarjan T, Gaurav G. Role of Omega Fatty Acids in Human Body. Asian J. Research Chem. 2(2): April.-June, 2009 page 9399.

  • Himanshu Joshi, Arun B Joshi, Hemlata Sati, Gururaja MP, Prajwal R Shetty, EVS Subrahmanyam, D Satyanaryana. Fatty Acids from Memecylon umbellatum (Burm.). Asian J. Research Chem. 2(2): April.-June, 2009 page 178-180.

  • Neelam Vaidya, Rakesh Choure. Electrochemical Analysis of Fatty Acids Obtained from the Natural Resource Seed of Perilla frutescens. Asian J. Research Chem. 4(5): May, 2011; Page 705707.

  • Gargee Yadav. Oil Content and Fatty Acid Variation in some Indian Accessions of Safflower (Carthamus tinctorious L.) Seed and Oil. Asian J. Research Chem. 5(10): October, 2012; Page 1289-1292.

  • Gargee Yadav, H.C. Srivastava. Fatty Acid Composition and Oil Content of Some Safflower (Carthamus tinctorius L.) Cultivars of Indian Origin. Asian J. Research Chem. 6(7): July 2013; Page 634-636.

  • Govindu Vani, Pranabesh Sikdar, J. Swathi Kiran, C. Pushpalatha, T. Sireesha, P. Reddy Rani, M. Vishnu Vardhan, M. Niranjan Babu. Evaluation of Physicochemical properties of some Edible

  • oils available in Tirupathi. Res. J. Pharmacognosy and Phytochem.2019; 11(3):179-185.

  • Savitha P., Saravana Kumar S.2. Effect of Omega-3 Fatty acids on Memory – Review. Research J. Pharm. and Tech. 7(6): June, 2014;Page 715-718.

  • DhiaF.Alfekaiki. Characteristics of Fat Milk Iraqi Buffalo (Bubalusbubalis). Research J. Pharm. and Tech 2018; 11(10):4349-4356.

  • R. Kamalambigeswari, S. Sharmila, E. Kowsalya, S. Selva Janani, V. Deva, L. Jeyanthi Rebecca. Extraction of Omega-3 Fatty Acid -methyl stearate from Soil Fungi (Fusarium sp.). Research J.

  • Pharm. and Tech 2019; 12(9):4295-4298.

  • Rania Matar, Mayssam Salami, Zaid Al Assaf. Factors affecting the Formation of Elaidic acid in Syrian Edible Oils during Frying with Home Conditions. Research J. Pharm. and Tech. 2019;

  • (11): 5451-5455.


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  • Risk assessment of different edible oils subjected to heat at different time intervals and analyzed by titrimetric and instrumental methods and biological estimation on Wistar albino rats

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Authors

M. Ramayyappa
Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, India
G. Raveendra Babu
Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, India
H.S.P. Syloosha
Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, India
V. Alekya Satyasri
Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, India
G. premi
Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, India
Ch. Prudhvi
Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, India

Abstract


Oils and fats in the diet serve three functions in the human body: as an energy source, a structural component, and as important biological regulators. Lipid peroxidation may be accelerated by repeatedly heating soy oil. Unsaturated fatty acids that have been oxidised may play a role in the aetiology of a variety of diseases. The purpose of this study was to see if repeatedly heating edible oil had any negative consequences on illness development. In this investigation, four different edible oils were heated to their regular boiling point and then heated for another 3 hours at a 1hour interval. The heated samples were subjected to qualitative examination. After analysing the results, it was discovered that saponification values declined as continuous heating, i.e. 3 hours, was applied to all different oils, resulting in low saponification values when compared to 2 hours, 1 hour, and normal boiling point. Lower the saponification value, the more free fatty acids and triglycerides are produced, which may contribute to cardio vascular illness. The acid values grew as the heating progressed, according to the findings. A high acid number suggests that there is a lot of acid in the system. The values of iodine fell as the heating progressed, according to the results. The presence of saturated fatty acids is indicated by a low iodine value. Saturated fatty acids were detrimental to one's health. As the heating progressed, the peroxide levels grew. Deterioration is indicated by an increase in peroxide value. When compared to other oils, palm oil showed a high saponification value, acid value, peroxide value, and low iodine value. Total cholesterol, tri-glycerides, and glucose levels were higher in 3 hours heated oils than in 2 hours, 1 hour, and at normal boiling point in biological tests .

Keywords


Edible oil, Heating, Fatty acids, Deterioration, Peroxide values.

References