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Estimation of Rosiglitazone in Bulk and Tablet Dosage Forms by HPTLC Method


Affiliations
1 Veerayatan Institute of Pharmacy, Bhuj-Mandvi Road, Jakhania, Mandvi-370460, India
2 Babaria Institute of Pharmacy, Vadodara-391240, India
3 Jiladia Hospital, New Anjar, Anjar (Kutch)-370110, India
     

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A simple and sensitive, HPTLC method has been developed for the quantitative estimation of rosiglitazone in its single component tablet formulations (4 mg). Rosiglitazone was chromatographed on silica Gel 60 F254 TLC plate using Toluene:acetone: ammonia (4.5:5.5:0.1 v/v) as mobile phase. Rosiglitazone showed Rf value 0.55±0.03 and scanned at 318 nm using Camag TLC Scanner 3. The method was validated in terms of linearity (200-2000 ng/spot), precision (intra-day variation 0.6 to 3.6%, inter-day variation 0.6 to 3.0%), accuracy (98.1 to 103.3%) and specificity. The limit of detection and limit of quantification for rosiglitazone were found to be 30 ng/spot and 100 ng/spot, respectively. The developed method was successfully used for the assay of rosiglitazone tablet formulations. The method is simple, sensitive and precise;it can be used for the routine quality control testing of marketed formulations.

Keywords

Rosiglitazone, Toluene, Acetone, Ammonia, HPTLC.
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  • Estimation of Rosiglitazone in Bulk and Tablet Dosage Forms by HPTLC Method

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Authors

Meeta A. Jiladia
Veerayatan Institute of Pharmacy, Bhuj-Mandvi Road, Jakhania, Mandvi-370460, India
S. S. Pandya
Babaria Institute of Pharmacy, Vadodara-391240, India
Ashok G. Jiladia
Jiladia Hospital, New Anjar, Anjar (Kutch)-370110, India
G. Vidyasagar
Veerayatan Institute of Pharmacy, Bhuj-Mandvi Road, Jakhania, Mandvi-370460, India

Abstract


A simple and sensitive, HPTLC method has been developed for the quantitative estimation of rosiglitazone in its single component tablet formulations (4 mg). Rosiglitazone was chromatographed on silica Gel 60 F254 TLC plate using Toluene:acetone: ammonia (4.5:5.5:0.1 v/v) as mobile phase. Rosiglitazone showed Rf value 0.55±0.03 and scanned at 318 nm using Camag TLC Scanner 3. The method was validated in terms of linearity (200-2000 ng/spot), precision (intra-day variation 0.6 to 3.6%, inter-day variation 0.6 to 3.0%), accuracy (98.1 to 103.3%) and specificity. The limit of detection and limit of quantification for rosiglitazone were found to be 30 ng/spot and 100 ng/spot, respectively. The developed method was successfully used for the assay of rosiglitazone tablet formulations. The method is simple, sensitive and precise;it can be used for the routine quality control testing of marketed formulations.

Keywords


Rosiglitazone, Toluene, Acetone, Ammonia, HPTLC.