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Development and Validation of Spectrophotometric Method for the Estimation of Lopinavir and Ritonavir in Tablet Dosage Form


Affiliations
1 College of Pharmacy, Kovai Medical Center Research and Educational Trust, Coimbatore-641048, India
2 Shantha Bio-Technics Limited, Hyderabad-501401, India
     

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A new, simple, rapid and novel spectrophotometric method has been developed for the simultaneous estimation of Lopinavir and Ritonavir using simultaneous equation. The method involved measurement of absorbance at two wavelengths, 259 nm and 240 nm, of Lopinavir and Ritonavir respectively. Beer’s law obeyed in concentration range of 10-50 μg/mL and 15-55 μg/mL for Lopinavir and Ritonavir respectively. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The method can be recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific. This paper describes the development and validation of ultra violet spectroscopic method for simultaneous estimation of Lopinavir and Ritonavir in combined solid dosage form.

Keywords

Lopinavir, Ritonavir, Simultaneous Equation Method, Beer’s Law.
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  • Development and Validation of Spectrophotometric Method for the Estimation of Lopinavir and Ritonavir in Tablet Dosage Form

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Authors

Ilangovan Ponnilavarasan
College of Pharmacy, Kovai Medical Center Research and Educational Trust, Coimbatore-641048, India
Aiyalu Rajasekaran
College of Pharmacy, Kovai Medical Center Research and Educational Trust, Coimbatore-641048, India
Joghee Gowder Dharuman
College of Pharmacy, Kovai Medical Center Research and Educational Trust, Coimbatore-641048, India
Duraisamy Kalaiyarasi
College of Pharmacy, Kovai Medical Center Research and Educational Trust, Coimbatore-641048, India
Duraisamy Krishnakumar
Shantha Bio-Technics Limited, Hyderabad-501401, India

Abstract


A new, simple, rapid and novel spectrophotometric method has been developed for the simultaneous estimation of Lopinavir and Ritonavir using simultaneous equation. The method involved measurement of absorbance at two wavelengths, 259 nm and 240 nm, of Lopinavir and Ritonavir respectively. Beer’s law obeyed in concentration range of 10-50 μg/mL and 15-55 μg/mL for Lopinavir and Ritonavir respectively. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The method can be recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific. This paper describes the development and validation of ultra violet spectroscopic method for simultaneous estimation of Lopinavir and Ritonavir in combined solid dosage form.

Keywords


Lopinavir, Ritonavir, Simultaneous Equation Method, Beer’s Law.