Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Development and Validation of Spectrophotometric Method for Simultaneous Estimation of Tramadol Hydrochloride and Paracetamol from their Combined Dosage Form


Affiliations
1 Shri B. M. Shah College of Pharmaceutical Education and Research, Modasa-383315, India
     

   Subscribe/Renew Journal


A Simple, accurate and precise method for simultaneous estimation of Tramadol Hydrochloride and Paracetamol in their combined dosage form have been described. The spectrophotometric method is based on zero absorbance measurement, which uses 272 and 250 nm as two analytical wavelengths. Beer’s law was obeyed in the concentration range of 60 to 160 μg/ml for Tramadol hydrochloride and 8 to 30 μg/ml for Paracetamol. The LOD and LOQ were found to be 25 μg/ml and 60 μg/ml for Tramadol hydrochloride and 3 μg/ml and 8 μg/ml for Paracetamol, respectively. The results obtained in the analysis of dosage form by developed method are well agreed with the labeled contents.

Keywords

Spectrophotometric, Tramadol Hydrochloride, Validation, Wavelength.
Subscription Login to verify subscription
User
Notifications
Font Size


Abstract Views: 170

PDF Views: 0




  • Development and Validation of Spectrophotometric Method for Simultaneous Estimation of Tramadol Hydrochloride and Paracetamol from their Combined Dosage Form

Abstract Views: 170  |  PDF Views: 0

Authors

Paresh B. Shah
Shri B. M. Shah College of Pharmaceutical Education and Research, Modasa-383315, India
Boni B. Shah
Shri B. M. Shah College of Pharmaceutical Education and Research, Modasa-383315, India

Abstract


A Simple, accurate and precise method for simultaneous estimation of Tramadol Hydrochloride and Paracetamol in their combined dosage form have been described. The spectrophotometric method is based on zero absorbance measurement, which uses 272 and 250 nm as two analytical wavelengths. Beer’s law was obeyed in the concentration range of 60 to 160 μg/ml for Tramadol hydrochloride and 8 to 30 μg/ml for Paracetamol. The LOD and LOQ were found to be 25 μg/ml and 60 μg/ml for Tramadol hydrochloride and 3 μg/ml and 8 μg/ml for Paracetamol, respectively. The results obtained in the analysis of dosage form by developed method are well agreed with the labeled contents.

Keywords


Spectrophotometric, Tramadol Hydrochloride, Validation, Wavelength.