Photodegradation Kinetic Study of Aceclofenac by HPTLC Method

A simple, specific and precise HPTLC method was developed and validated for estimation of aceclofenac in photodegraded sample. The method involves separation of sample and photodegradation product on TLC aluminum sheets pre-coated with silica gel 60 F₂₅₄ plate using a mixture of Toluene:Ethyl acetate:Glacial acetic acid:Methanol (6.00:1.00:0.20:0.15 v/v/v/v) as the mobile phase. Detection of the spot was carried out at 284 nm in absorbance/reflectance mode. The retardation factor (R_f) for aceclofenac was found to be 0.35±0.03. The linearity range was found to be 50-150 ng/spot with r²=0.9931. The validated method was applied for determination of photodegradation kinetic study. Photodegradation reaction at various experimental conditions follows zero order kinetic and rate constant (K) was determined to be 0.4855 ng μl^-1 min^-1. T_1/2 and T_90% was found to be 116.78 min^-1 and 23.35 min^-1 respectively.