Asian Journal of Research in Chemistry

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UV Spectrophotometric Method for the Determination of Gemifloxacin in Bulk and Pharmaceutical Formulation


Affiliations
1 Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India
     

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A simple, sensitive and reproducible spectrophotometric method was developed for the determination of Gemifloxacin (GEMI) in pure and dosage forms. It has absorption maximum at 343 nm and obeys beer’s law in the concentration range 2-20 μg mL-1 .Results of analysis were validated statistically and by recovery studies .The apparent molar absorptivity and sandell’s sensitivity were 1.3862×104 L mol-1 cm-1 and 2.809×10-2 μg cm-2, respectively. The slope and intercept of the equation of the regression line are 0.05370 and -0.00640 respectively. Correlation coefficient was found to be 0.999. This method is successfully employed for the determination of Gemifloxacin in pharmaceutical preparation.
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  • UV Spectrophotometric Method for the Determination of Gemifloxacin in Bulk and Pharmaceutical Formulation

Abstract Views: 150  |  PDF Views: 0

Authors

S. Ganapathy
Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India
D. G. Sankar
Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India
Pettla Y. Naidu
Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India

Abstract


A simple, sensitive and reproducible spectrophotometric method was developed for the determination of Gemifloxacin (GEMI) in pure and dosage forms. It has absorption maximum at 343 nm and obeys beer’s law in the concentration range 2-20 μg mL-1 .Results of analysis were validated statistically and by recovery studies .The apparent molar absorptivity and sandell’s sensitivity were 1.3862×104 L mol-1 cm-1 and 2.809×10-2 μg cm-2, respectively. The slope and intercept of the equation of the regression line are 0.05370 and -0.00640 respectively. Correlation coefficient was found to be 0.999. This method is successfully employed for the determination of Gemifloxacin in pharmaceutical preparation.