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Study on Tri Calcium Phosphate Solubilisation Efficiency of Soil Microbes


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1 Ultra College of Pharmacy, Madurai, India
     

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Different soil microbes were analyzed for their phosphate solubilizing capacity. The characterization of the microbes which exhibits phosphate solubilisation in preliminary screening reveals that the isolated strains were Pseudomonas aeroginosa, Pseudomonas flurosence, Bacillus subtilis, Bacillus cereus and E. coli. General parameters for determining phosphate solubilisation in PVK broth such as pH changes, estimation of phosphorus release in media and the phosphatase enzyme activity were performed. Investigation results showed high potency of the microbes to solubilize TCP. From the above tested organisms Pseudomonos pocess high efficiency to solubilize TCP followed by Bacillus and E.coli on tested all parameters. Biofertilizer was developed and nursery experiments were conducted with paddy crop. Parameters such as Germination of seed, Color of the crop and Height were analyzed. Biofertilizer treated crop displayed comparatively good results with non treated and chemical fertilizer treated crop. In conclusion Pseudomonas and Bacillus strains showed good phosphate solubilisation and can be utilized in biofertilizers.

Keywords

Phosphate Solubilization, PVK Broth, Pseudomonas, Bacillus.
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  • Study on Tri Calcium Phosphate Solubilisation Efficiency of Soil Microbes

Abstract Views: 150  |  PDF Views: 0

Authors

K. Natarajan
Ultra College of Pharmacy, Madurai, India
S. Tamilarasi
Ultra College of Pharmacy, Madurai, India
R. Sathish
Ultra College of Pharmacy, Madurai, India
C. Amudhadevi
Ultra College of Pharmacy, Madurai, India
T. Regupathi
Ultra College of Pharmacy, Madurai, India

Abstract


Different soil microbes were analyzed for their phosphate solubilizing capacity. The characterization of the microbes which exhibits phosphate solubilisation in preliminary screening reveals that the isolated strains were Pseudomonas aeroginosa, Pseudomonas flurosence, Bacillus subtilis, Bacillus cereus and E. coli. General parameters for determining phosphate solubilisation in PVK broth such as pH changes, estimation of phosphorus release in media and the phosphatase enzyme activity were performed. Investigation results showed high potency of the microbes to solubilize TCP. From the above tested organisms Pseudomonos pocess high efficiency to solubilize TCP followed by Bacillus and E.coli on tested all parameters. Biofertilizer was developed and nursery experiments were conducted with paddy crop. Parameters such as Germination of seed, Color of the crop and Height were analyzed. Biofertilizer treated crop displayed comparatively good results with non treated and chemical fertilizer treated crop. In conclusion Pseudomonas and Bacillus strains showed good phosphate solubilisation and can be utilized in biofertilizers.

Keywords


Phosphate Solubilization, PVK Broth, Pseudomonas, Bacillus.