Asian Journal of Research in Chemistry

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Studies on DNA Extraction, Molecular Identification and Genetic Evolution of Trichoderma harzianum


Affiliations
1 Dept of Botany and Microbiology, A. V. V. M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) - 613 503, Tamil Nadu, India
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, India
     

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The chromosomal DNA was isolated from T. harzianum and PCR amplification was performed. The 18S rDNA gene sequencing was made by using ITS (internal transcribed spacer) method. The 18S rDNA gene sequences were submitted to Genebank, NCBI, the accession number GU646678 has been obtained. The phylogenetic tree was constructed using MEGA 4 (Molecular Evolutionary Genetics Analysis Software version 4.0). Based on the neighbour joining tree analysis the isolate T. harzianum has been found closely related to already existing species of Hypocera tixii at 99 per cent of similarity.

Keywords

Trichoderma harzianum, DNA Isolation, Evolution.
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  • Studies on DNA Extraction, Molecular Identification and Genetic Evolution of Trichoderma harzianum

Abstract Views: 182  |  PDF Views: 0

Authors

G. Senthilkumar
Dept of Botany and Microbiology, A. V. V. M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) - 613 503, Tamil Nadu, India
P. Madhanraj
Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, India
A. Panneerselvam
Dept of Botany and Microbiology, A. V. V. M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) - 613 503, Tamil Nadu, India

Abstract


The chromosomal DNA was isolated from T. harzianum and PCR amplification was performed. The 18S rDNA gene sequencing was made by using ITS (internal transcribed spacer) method. The 18S rDNA gene sequences were submitted to Genebank, NCBI, the accession number GU646678 has been obtained. The phylogenetic tree was constructed using MEGA 4 (Molecular Evolutionary Genetics Analysis Software version 4.0). Based on the neighbour joining tree analysis the isolate T. harzianum has been found closely related to already existing species of Hypocera tixii at 99 per cent of similarity.

Keywords


Trichoderma harzianum, DNA Isolation, Evolution.