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Determination of Buclizine Hydrochloride by Derivative Spectrophotometry and RP-HPLC in Tablet Dosage Form


Affiliations
1 Department of Pharmaceutical Analysis, J. K. K. Nattraja College of Pharmacy, Komarapalayam – 638 183, Namakkal Dt. Tamil Nadu, India
2 Department of Pharmaceutical Chemistry, J. K. K. Nattraja College of Pharmacy, Komarapalayam – 638 183, Namakkal Dt. Tamil Nadu, Iceland
3 Dep. of Pharmaceutical Chemistry, Swamy Vivekanandha College of Pharmacy, Elayampalayam – 637 205, Namakkal Dt. Tamil Nadu, India
     

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A simple and specific UV derivative spectroscopy and RP-HPLC was developed for buclizine hydrochloride. These methods are carried out by using 234 nm as the wavelength and 100% v/v methanol as solvent. Second derivative spectroscopy was developed to minimize the interferences. The linearity ranges were 10-50 mcg/ml for derivative spectroscopy and 1-10 mcg/ml for the RP-HPLC respectively. Chromatographic separation was made on Phenomenex Luna C18 (250 mm × 4.6 mm, 5μ) column at room temperature with 1.0 ml/min as flow rate and the injection volume was 20 μl. The retention time was found to be 7.15 min. The high recovery and low relative standard deviation confirms the suitability of the methods for the determination of buclizine hydrochloride in pharmaceutical dosage forms.

Keywords

Buclizine Hydrochloride, UV Derivative Spectroscopy, RP-HPLC, Piperazine Derivative, Antiemetic.
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  • Determination of Buclizine Hydrochloride by Derivative Spectrophotometry and RP-HPLC in Tablet Dosage Form

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Authors

G. Babu
Department of Pharmaceutical Analysis, J. K. K. Nattraja College of Pharmacy, Komarapalayam – 638 183, Namakkal Dt. Tamil Nadu, India
Thejonath Kolla
Department of Pharmaceutical Analysis, J. K. K. Nattraja College of Pharmacy, Komarapalayam – 638 183, Namakkal Dt. Tamil Nadu, India
K. Prasad
Department of Pharmaceutical Analysis, J. K. K. Nattraja College of Pharmacy, Komarapalayam – 638 183, Namakkal Dt. Tamil Nadu, India
M. Vijayabaskaran
Department of Pharmaceutical Chemistry, J. K. K. Nattraja College of Pharmacy, Komarapalayam – 638 183, Namakkal Dt. Tamil Nadu, Iceland
S. T. Latha
Dep. of Pharmaceutical Chemistry, Swamy Vivekanandha College of Pharmacy, Elayampalayam – 637 205, Namakkal Dt. Tamil Nadu, India

Abstract


A simple and specific UV derivative spectroscopy and RP-HPLC was developed for buclizine hydrochloride. These methods are carried out by using 234 nm as the wavelength and 100% v/v methanol as solvent. Second derivative spectroscopy was developed to minimize the interferences. The linearity ranges were 10-50 mcg/ml for derivative spectroscopy and 1-10 mcg/ml for the RP-HPLC respectively. Chromatographic separation was made on Phenomenex Luna C18 (250 mm × 4.6 mm, 5μ) column at room temperature with 1.0 ml/min as flow rate and the injection volume was 20 μl. The retention time was found to be 7.15 min. The high recovery and low relative standard deviation confirms the suitability of the methods for the determination of buclizine hydrochloride in pharmaceutical dosage forms.

Keywords


Buclizine Hydrochloride, UV Derivative Spectroscopy, RP-HPLC, Piperazine Derivative, Antiemetic.