Asian Journal of Pharmaceutical Analysis

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Simultaneous Estimation of Metformin Glimeperide and Voglibose by RP-UPLC


Affiliations
1 Department of Pharmaceutical Chemistry, Aditya Pharmacy College, Surampalem, Andhra Pradesh, India
2 Nitte University, NGSMIPS, Department of Pharmaceutical Chemistry, Mangalore, Karnataka, India
     

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A simple, sensitive and precise RP-UPLC method for estimation of Metformin, Glimeperide and Voglibose has been developed and validated for determination of compounds in commercial tablet dosage form. The compounds were well separated isocratically on a Inertsil ODS column using a mobile phase consisting of buffer (pH 3), and Methanol in the ratio of 70:30 v/v 0.4 ml/min flow rate with PDA detector. Retention time for Metformin, Glimeperide and Voglibose was found to be 0.903, 2.619, 3.818 min respectively. The method was validated in accordance with ICH guidelines. The study showed that the reverse phased liquid chromatography was sensitive and selective for detecting Metformin, Glimeperide and Voglibose using the single mobile phase.

Keywords

Metformin, Glimeperide, Voglibose Ultra Performance Liquid Chromatography, Validation, Degaradation.
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  • Simultaneous Estimation of Metformin Glimeperide and Voglibose by RP-UPLC

Abstract Views: 195  |  PDF Views: 1

Authors

D. Sri Lakshmi
Department of Pharmaceutical Chemistry, Aditya Pharmacy College, Surampalem, Andhra Pradesh, India
Jane T. Jacob
Nitte University, NGSMIPS, Department of Pharmaceutical Chemistry, Mangalore, Karnataka, India
D. Srinivasa Sastry
Department of Pharmaceutical Chemistry, Aditya Pharmacy College, Surampalem, Andhra Pradesh, India
D. Satyanarayana
Department of Pharmaceutical Chemistry, Aditya Pharmacy College, Surampalem, Andhra Pradesh, India

Abstract


A simple, sensitive and precise RP-UPLC method for estimation of Metformin, Glimeperide and Voglibose has been developed and validated for determination of compounds in commercial tablet dosage form. The compounds were well separated isocratically on a Inertsil ODS column using a mobile phase consisting of buffer (pH 3), and Methanol in the ratio of 70:30 v/v 0.4 ml/min flow rate with PDA detector. Retention time for Metformin, Glimeperide and Voglibose was found to be 0.903, 2.619, 3.818 min respectively. The method was validated in accordance with ICH guidelines. The study showed that the reverse phased liquid chromatography was sensitive and selective for detecting Metformin, Glimeperide and Voglibose using the single mobile phase.

Keywords


Metformin, Glimeperide, Voglibose Ultra Performance Liquid Chromatography, Validation, Degaradation.