Asian Journal of Home Science

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Assessment of Milk Clotting Activities of Plant Latex


Affiliations
1 Department of Home Science (food and Nutrition) Banaras Hindu University, Varanasi, U.P., India
2 Department of Medicinal Chemistry, Institute of Medical Sciences (b.h.u.) Varanasi, U.P., India
     

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A number of proteolytic enzymes are widely employed in food industries for cheese manufacture. The coagulant which is widely used in cheese production or manufacturing of cheese is animal rennet, which contains chymosin, an asparatic protease which is responsible for milk clotting. Thus, there's a need for the identification of a milk clotting enzyme from other sources that can meet the industrial demand of rennet. In this context, the present investigation was carried out in order to extract a milk clotting enzyme from different plant sources which can be utilized in cheese production. Three plants, Carica papaya, Euphorbia splendens and Musa paradasica belonging to the families Annonaceace, Euphorbiaceae and Musaceae were examined for the presence of proteinases in the present study. The proteinase activity was estimated by determining the milk clotting property of crude latex by identifying specific activity through their partial purification. The results indicated that Carica papaya plant latex had the highest milk clotting activity (2006.20 3.77 U/ ml) than Musa paradasica (187.19 4.44U/ml) and minimum in Europhorbia splendens (105.20 4.15 U/ ml) (p =0.01). SDS-PAGE analysis of the plant latex exhibiting maximum milk clotting activity displayed 5 bands ranging from 14.3 KD to 97.4 KD.

Keywords

Milk Clotting Enzyme, Sds-page, Plant Latex, Carica Papaya, Musa Paradasica, Euphorbia Splendens
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  • Abdalla, M.O.M., Ali, D.A. and Mohamed, B.E. (2010). Extraction, milk clotting activity measurements and purification ofSolanum dubium Fresen (Gubbain) for cheese making.World J. Dairy Food Sci., 5(2): 152-159.

  • Ahmed, I.A.M., Morishima, I., Babiker, E.E. and N, Mori. (2009). Characterisation of partial purified milk-clotting enzyme fromSolanum dubium Fresen seeds. Food Chem., 116(2):395-400.

  • Alirezaei, M., Aminlari, M., Gheisari, H.R. and Tavana, M. (2011). Actinidin: Promising milk coagulating enzyme.Eur. J. Food Res. Rev., 1(2): 43-51.

  • Asakura, T., Watanabe, H., Abe, K. and Arai, S. (1997). Oryzasin is an aspartic proteinase occurring in rice seeds: purification, characterization and application of milk clotting. J. Agric. Food Chem., 45 (4) : 1070-1075.

  • Badgujar, S.B. and Mahajan, R.T. (2009). Proteolytic enzymes of some lacticiferous plant belonging to Khandesh region of Maharashtra, India. J. Pharm. Res., 2(9): 1434-1437.

  • Dahot, M.U., Khan, M.Y. and Memon, A.N. (1990). Screening of some Pakistani plants for milk clotting activity. J. Islamic Acad. Sci., 3(4): 284-286.

  • Duarte, A.R., Duarte, D. M. R., Moreira, K. A., Cavalcanti, M. T. H., Lima-Filho, J.L.de and Porto, A.L.F. (2009). Jacartia corumbensis O. Kuntze, a new vegetable source of milk clotting enzymes. Braz. Arch. Biol. Technol.,52(1): 1-9.

  • Esteves, C.L.C., Lucey, J.A., Wang, T. and Pires, E.M.V. (2003). Effect of pH on the gelation properties of skim milk gels made from plant coagulants and chymosin. J. Dairy Sci.,86 (8) : 2558-2567.

  • Guiama, V.D., Libouga, D.G., Ngah, E. and Mbofung, C.M. (2010). Milk-clotting activity of berries extracts from nine Solanum plants. Afr. J. Biotechnol., 9 (25): 3911-3918.

  • Hooydonk,A.C.M.and Walstras,P.(1987).Interpretation of the kinetics of the renneting reaction in milk. Neth. Milk DairyJ.,41:19-47.

  • Kumari, M., Sharma, A. and Jagannadham, M.V. (2010). Decolorization of crude latex by activated charcoal, purification and physio-chemical characterization of religiosin, a milk-clotting serine protease from the latex of Ficus religiosa. J. Agric. Food Chem., 58(13): 8027-8034.

  • Laemmli, U.K. (1970). Cleavage of structural protein during the assembly of head of bacteriophage T4. Nature, 227: 680-685.

  • Liggieri,C.,Obregon,W.,Trejo,S. and Priolo, N. (2009). Biochemical analysis of a papain like protease isolated from the latex of Asclepias curassavica L. Acta Biochem Biophys. Sin., 41(2): 154-162.

  • Lo Piero, A.R., Puglisi, I. and Petrone, G. (2002). Characterisation of Lettucine, a serine like protease from Latuca sativa leaves, as a novel enzyme for milk clotting. J. Agric. Food Chem., 50 (8) : 2439-2443.

  • Lopes, A., Teixeira, G., Liberato, M.C., Pais, M.S. and Clemente, A. (1998). New vegetal sources of milk clotting enzyme. J. Mol. Catal. B Enzymatic., 5 (1-4) : 63-68.

  • Macedo, I.Q., Faro, C.J. and Pires, E.M. (1993). Specificity and kinetics of the milk-clotting enzyme from Cardoon (Cynara cardunculus L.) toward bovine ?appa -casein. J. Agric. Food Chem., 41: 1537-1540.

  • Najera, A.I., de Renobales, M. and Barron, L.J.R. (2003). Effects of pH, temperature, CaCl2 and enzyme concentrations on the rennetclotting properties of milk: a multifactorial study. Food Chem., 80(3): 345-352.

  • Nestor, G.M., Rubi, C.D. and Hector, J.C. (2011). Exploring the milk clotting properties of a plant coagulant from the berries of S. elaeagnifolium var. cavanilles. J. Food Sci., 77(1): C89-94.

  • Nitsawang, S., Hatti Kaul, R. and Kanasawud, P. (2006). Purification of papain fromCarica papaya latex: Aqueous two phase extraction versus two step salt precipitation. Enz. Microb. Technol., 39 (5) : 1103-1107.

  • Nouani, A., Dako, E., Morsli, A., Belhamiche, N., Belbraouet, S., Bellal, M.M. and Dadie, A. (2009). Characterization of the purified coagulant extracts derived from Artichoke Flowers (Cynara scolymus) and from the fig tree latex (Ficus carica) in light of their use in the manufacturing of traditional cheeses in Algeria. J. Food Technol., 7(1): 20-29.

  • Roseiro, L.B., Barbosa, M., Ames, J.M. and Wilbey, R.A. (2003). Cheese making with vegetable coagulants the use of Cynara L. for the production of ovine milk cheeses. Internat. J. Dairy Technol., 56 (2): 76-85.

  • Silva, L.G., Garcia, O., Lopes, M.T.P. and Salas, C.E. (1997). Changes in protein profile during coagulation of latex from Carica papaya. Braz J. Med. Biol. Res., 30(5): 615-619.

  • Silva, S.V. and Malcata, F.X. (2000). Comparative catalytic activity of two plant proteinases upon Caprine caseins in solution.Food Chem., 71 (2) : 207-214.

  • Silva, S.V. and Malcata, F.X. (2005). Studies pertaining to coagulant and proteolytic activities of plant proteases fromCynara cardunculus. Food Chem., 89 (1) : 19-26.

  • Uchikoba, T. and Kadena, M. (1996). Milk clotting activity of cucumisin, a plant serine protease from melon fruit. Appl. Biochem. Biotechnol., 56 (3) : 325-330.

  • Visser, S. (1993). Proteolytic enzymes and their relation to cheese ripening and flavor: An overview. J. Dairy Sci., 76 (1) : 329-350.

  • Yousif, B.H., Mc Mahon, D.J. and Shammet, K.M. (1996). Milk clotting enzyme from Solanum dobium plant. Internat. Dairy J., 6 (6) : 637-644.

  • Yadav, S.C., Pande, M. and Jagannadham, M.V. (2006). Highly stable glycosylated serine protease from the medicinal plantEuphorbia milii. Phytochemistry., 67 (14) : 1414-1426.


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  • Assessment of Milk Clotting Activities of Plant Latex

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Authors

Upasana
Department of Home Science (food and Nutrition) Banaras Hindu University, Varanasi, U.P., India
Archana Chakravarty
Department of Home Science (food and Nutrition) Banaras Hindu University, Varanasi, U.P., India
Adya Chaturvedi
Department of Medicinal Chemistry, Institute of Medical Sciences (b.h.u.) Varanasi, U.P., India
Y. B. Tripathi
Department of Medicinal Chemistry, Institute of Medical Sciences (b.h.u.) Varanasi, U.P., India

Abstract


A number of proteolytic enzymes are widely employed in food industries for cheese manufacture. The coagulant which is widely used in cheese production or manufacturing of cheese is animal rennet, which contains chymosin, an asparatic protease which is responsible for milk clotting. Thus, there's a need for the identification of a milk clotting enzyme from other sources that can meet the industrial demand of rennet. In this context, the present investigation was carried out in order to extract a milk clotting enzyme from different plant sources which can be utilized in cheese production. Three plants, Carica papaya, Euphorbia splendens and Musa paradasica belonging to the families Annonaceace, Euphorbiaceae and Musaceae were examined for the presence of proteinases in the present study. The proteinase activity was estimated by determining the milk clotting property of crude latex by identifying specific activity through their partial purification. The results indicated that Carica papaya plant latex had the highest milk clotting activity (2006.20 3.77 U/ ml) than Musa paradasica (187.19 4.44U/ml) and minimum in Europhorbia splendens (105.20 4.15 U/ ml) (p =0.01). SDS-PAGE analysis of the plant latex exhibiting maximum milk clotting activity displayed 5 bands ranging from 14.3 KD to 97.4 KD.

Keywords


Milk Clotting Enzyme, Sds-page, Plant Latex, Carica Papaya, Musa Paradasica, Euphorbia Splendens

References