Asian Journal of Bio Science

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Molecular Characterization and Identification of an Alkalophilic Bacterial Strain Isolated from a Local Hotspring Atri, Khurda District, Odisha, India


Affiliations
1 Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneshwar, Orissa, India
2 First Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyanga, Bhubaneshwar, Orissa, India
     

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The present study was conducted to isolate, identify, characterize and to determine the enzymatic activities of the thermophiles from Atri hot spring of odisha. The optimal temperature for growth of these isolates was 55 C and the optimal pH was 8. These bacterial cells were Gram positive rods and endospore forming. All the strains were amylase, catalase and oxidase positive but gelatinase and caseinase negative but isolate A1 showed the best amylase producing. The phenotypic characterization of those isolates was confirmed by genotypic method using 16S rDNA sequence analysis. Maximal homology of isolate A1 to genus Geobacillus was observed. Isolate A1 showed 96 per cent homology with Geobacillus sp. WCH 70 (Accession no. NC 012793.1). Therefore, 16S rDNA gene sequence analysis can be considered as a valuable genotypic tool for the identification and characterization of this thermophilic bacterium at genus level. Moreover, enzymatic products of these isolates could receive considerable attention due to their potential applications in biotechnology.

Keywords

Thermophiles, Enzymes, Hot Spring, 16S rDNA.
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  • Molecular Characterization and Identification of an Alkalophilic Bacterial Strain Isolated from a Local Hotspring Atri, Khurda District, Odisha, India

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Authors

S. P. Nayak
Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneshwar, Orissa, India
P. Ray
Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneshwar, Orissa, India
P. K. Sahoo
First Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyanga, Bhubaneshwar, Orissa, India

Abstract


The present study was conducted to isolate, identify, characterize and to determine the enzymatic activities of the thermophiles from Atri hot spring of odisha. The optimal temperature for growth of these isolates was 55 C and the optimal pH was 8. These bacterial cells were Gram positive rods and endospore forming. All the strains were amylase, catalase and oxidase positive but gelatinase and caseinase negative but isolate A1 showed the best amylase producing. The phenotypic characterization of those isolates was confirmed by genotypic method using 16S rDNA sequence analysis. Maximal homology of isolate A1 to genus Geobacillus was observed. Isolate A1 showed 96 per cent homology with Geobacillus sp. WCH 70 (Accession no. NC 012793.1). Therefore, 16S rDNA gene sequence analysis can be considered as a valuable genotypic tool for the identification and characterization of this thermophilic bacterium at genus level. Moreover, enzymatic products of these isolates could receive considerable attention due to their potential applications in biotechnology.

Keywords


Thermophiles, Enzymes, Hot Spring, 16S rDNA.

References