Asian Journal of Bio Science

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Accessing Genetic Variability in Chickpea (Cicer arietinum L.) Varieties Differing in Susceptibility To Fusarium oxysporum F.sp. ciceri Using Issr Markers


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1 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, India
     

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Due to Fusarium wilt the production of chickpea has been affected in large. Molecular variability encompassed in chickpea offers an efficient means of exploiting disease resistance variety. The DNA-based molecular marker technique, inter simple sequence repeat (ISSR) was used to study the genetic variability for 5 chickpea varieties differing in their susceptibility to wilt disease using a different ISSR primers in present investigation. From the sixteen ISSR primer used, only two primers, primer 6956-022 (sequence of (GA)8 CTC) and 6956-030 ((AC)8 YG) gave three specific amplicon viz.,167,191, 272 and 196, 249, 408 base pair (bp) respectively, for resistant variety WR-315. Similarly, 6956-029 (146 bp), 6956-022 (311 bp), 6956-038 (1157 bp), 6956-031 (119, 139 bp) and 6956-033 (310 bp) markers associated with the chickpea variety JCP-27. For susceptible variety JG-62, three ISSR primer of a sequence (GA)8 T, (AG)8YT and (AC)8CCTA generated unique band for DNA produced 1343, 534 and 231 bp markers, respectively.

Keywords

Chickpea, Fusarium Oxysporum F.sp. Ciceri, Issr
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  • Accessing Genetic Variability in Chickpea (Cicer arietinum L.) Varieties Differing in Susceptibility To Fusarium oxysporum F.sp. ciceri Using Issr Markers

Abstract Views: 417  |  PDF Views: 1

Authors

U. K. Kandoliya
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, India
D. N. Vakharia
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, India

Abstract


Due to Fusarium wilt the production of chickpea has been affected in large. Molecular variability encompassed in chickpea offers an efficient means of exploiting disease resistance variety. The DNA-based molecular marker technique, inter simple sequence repeat (ISSR) was used to study the genetic variability for 5 chickpea varieties differing in their susceptibility to wilt disease using a different ISSR primers in present investigation. From the sixteen ISSR primer used, only two primers, primer 6956-022 (sequence of (GA)8 CTC) and 6956-030 ((AC)8 YG) gave three specific amplicon viz.,167,191, 272 and 196, 249, 408 base pair (bp) respectively, for resistant variety WR-315. Similarly, 6956-029 (146 bp), 6956-022 (311 bp), 6956-038 (1157 bp), 6956-031 (119, 139 bp) and 6956-033 (310 bp) markers associated with the chickpea variety JCP-27. For susceptible variety JG-62, three ISSR primer of a sequence (GA)8 T, (AG)8YT and (AC)8CCTA generated unique band for DNA produced 1343, 534 and 231 bp markers, respectively.

Keywords


Chickpea, Fusarium Oxysporum F.sp. Ciceri, Issr

References