Asian Journal of Bio Science

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Optimization of β-Glucosidase Assay and Protein Estimation from Various Parts of Rauvolfia serpentina


Affiliations
1 Allahabad Agricultural Institute (Deemed University) Allahabad (U.P.), India
     

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Rauvolfia serpentina is gifted with unique alkaloids that have remarkable medicinal properties and is being pronounced as “Wonder drug of India”. Several Ayurvedic preparations containing Rauvolfia plant parts are available in the market. The activity of crude enzyme in Rauvolfia serpentina was highest in mature leaf-1 (100%) followed by very young leaf (94.715%), whereas lowest in ischolar_main (9.829%). It was noticed that very young leaf and young leaf have highest protein 7.4573 and 3.8344 mg/ml, respectively and lowest protein content was found in stem (0.1689 mg/ml) of Rauvolfia serpentina. Young leaf contained highest number of isoforms (3).

Keywords

Rauvolfia serpentina, Protein, Alkaloid, β-Glucosidase.
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  • Optimization of β-Glucosidase Assay and Protein Estimation from Various Parts of Rauvolfia serpentina

Abstract Views: 162  |  PDF Views: 0

Authors

Om Prakash Verama
Allahabad Agricultural Institute (Deemed University) Allahabad (U.P.), India
Ankit Kumar
Allahabad Agricultural Institute (Deemed University) Allahabad (U.P.), India
Nirmala Singh
Allahabad Agricultural Institute (Deemed University) Allahabad (U.P.), India
Subhash Chandra Verma
Allahabad Agricultural Institute (Deemed University) Allahabad (U.P.), India
Purushottum Kumar
Allahabad Agricultural Institute (Deemed University) Allahabad (U.P.), India

Abstract


Rauvolfia serpentina is gifted with unique alkaloids that have remarkable medicinal properties and is being pronounced as “Wonder drug of India”. Several Ayurvedic preparations containing Rauvolfia plant parts are available in the market. The activity of crude enzyme in Rauvolfia serpentina was highest in mature leaf-1 (100%) followed by very young leaf (94.715%), whereas lowest in ischolar_main (9.829%). It was noticed that very young leaf and young leaf have highest protein 7.4573 and 3.8344 mg/ml, respectively and lowest protein content was found in stem (0.1689 mg/ml) of Rauvolfia serpentina. Young leaf contained highest number of isoforms (3).

Keywords


Rauvolfia serpentina, Protein, Alkaloid, β-Glucosidase.