Asian Journal of Bio Science

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Phylogenetic Relationships of Pigeonpea (Cajanus cajan) and its wild Relatives based on RAPD Markers


Affiliations
1 Plant Molecular Genetics Laboratory, Division of Biotechnology, M.I.T.S., Rayagada (Orissa), India
2 Department of Agricultural Botany, Marathwada Agricultural University, Parbhani (M.S.), India
3 Indira Gandhi Agriculture University, Raipur (C.G.), India
     

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In the present investigation RAPD marker was used for the elucidation of genetic relationships in the genus Cajanus and genetic fingerprinting of pigeonpea cultivars as well as wild species of Cajanus. RAPD markers utilized for the identification of pigeonpea,   cultivars (DSLR-17, BDN-2, ICWR-03 and ICWR-12) and ten wild species, including C. cajanifolius, C. lineatus, C. sericeus, C. acutifolius, C. lanceolatus, C. reticulates, C. albicans, C. scarabaeoides, C. volubilis and C. platycarpus, using a set of 10 primers were found to be polymorphic at species level and generated 85 unequivocal scorable polymorphic bands. The size of amplification products ranges from 102 bp to 2854 bp. The present study accentuates upon the utility of RAPD markers for the identification of cultivars of pigeonpea and allied species of C. cajan. The inter/ intra specific genetic variability studies based on RAPD marker showed a large amount of genetic variation between the species of Cajanus and their clustering pattern partially, supported the sectional classification. It was hypothesised that both C. cajan and C. cajanifolius might be derived from a common ancestor and experienced minor genomic rearrangement during divergence.

Keywords

Cajanus, Pigeonpea, RAPD, Proximity Matrix Analysis.
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  • Phylogenetic Relationships of Pigeonpea (Cajanus cajan) and its wild Relatives based on RAPD Markers

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Authors

Deep Ratan Kumar
Plant Molecular Genetics Laboratory, Division of Biotechnology, M.I.T.S., Rayagada (Orissa), India
Y. A. Deshmukh
Department of Agricultural Botany, Marathwada Agricultural University, Parbhani (M.S.), India
Tejbhan Saini
Indira Gandhi Agriculture University, Raipur (C.G.), India

Abstract


In the present investigation RAPD marker was used for the elucidation of genetic relationships in the genus Cajanus and genetic fingerprinting of pigeonpea cultivars as well as wild species of Cajanus. RAPD markers utilized for the identification of pigeonpea,   cultivars (DSLR-17, BDN-2, ICWR-03 and ICWR-12) and ten wild species, including C. cajanifolius, C. lineatus, C. sericeus, C. acutifolius, C. lanceolatus, C. reticulates, C. albicans, C. scarabaeoides, C. volubilis and C. platycarpus, using a set of 10 primers were found to be polymorphic at species level and generated 85 unequivocal scorable polymorphic bands. The size of amplification products ranges from 102 bp to 2854 bp. The present study accentuates upon the utility of RAPD markers for the identification of cultivars of pigeonpea and allied species of C. cajan. The inter/ intra specific genetic variability studies based on RAPD marker showed a large amount of genetic variation between the species of Cajanus and their clustering pattern partially, supported the sectional classification. It was hypothesised that both C. cajan and C. cajanifolius might be derived from a common ancestor and experienced minor genomic rearrangement during divergence.

Keywords


Cajanus, Pigeonpea, RAPD, Proximity Matrix Analysis.