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Effect of Growth Media pH and Incubation Temperatures on the Production of Proteinases From Bacillus sp. 26


Affiliations
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), India
     

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Bacillus sps-26 isolated and characterized from dairy effluents was challenged for its ability to produce alkaline proteinase using different temperature and pH as bioparameters with a chemically defined medium containing 5% casein. The maximum enzyme production was achieved at 30.C at pH 10, which was assayed using Genov method. Although moderate enzyme level was obtained at 10.C, it can pave the way for its affordable use in detergent industries.

Keywords

Bacillus sp-26, Alkaline Proteinase, Bioparameters, Tseushida Method, Lowry Method.
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  • Effect of Growth Media pH and Incubation Temperatures on the Production of Proteinases From Bacillus sp. 26

Abstract Views: 190  |  PDF Views: 0

Authors

R. Anand
Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), India
R. Aswini
Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), India
V. H. Mary Jiji
Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), India
V. Keerthana
Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), India

Abstract


Bacillus sps-26 isolated and characterized from dairy effluents was challenged for its ability to produce alkaline proteinase using different temperature and pH as bioparameters with a chemically defined medium containing 5% casein. The maximum enzyme production was achieved at 30.C at pH 10, which was assayed using Genov method. Although moderate enzyme level was obtained at 10.C, it can pave the way for its affordable use in detergent industries.

Keywords


Bacillus sp-26, Alkaline Proteinase, Bioparameters, Tseushida Method, Lowry Method.