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Development of Triploid Plantlets from Immature Endosperm of Garcinia gummi-gutta var. gummigutta (L) Rob


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1 Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Kerala Agricultural University, Vellanikkara, Thrissur (Kerala), India
     

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The inoculated immature endosperm bits of 1cm size onto ½ MS supplemented with 3.0 mgl-1 each of BA and Kinetin induced adventitious buds and subsequent proliferation of multiple shoots. These triploid plantlets were ischolar_mained, hardened and planted out. Development of regeneration protocol from triploid parenchymatous tissue of endosperm not only overcome the dearth of demands for true-to-type propagules but also surpassed the gender dimorphism. Evolving seedlessness through in vitro endosperm culture may also be helpful to avoid the processing difficulty and to support the establishment of domestic HCA extraction unit for Women empowerment.

Keywords

Garcinia gummi-gutta Var. Gummigutta, Polygamodioecious, Kodampuli, Immature Seeds, Endosperm Culture, Triploid, Seedlessness, Life Style Diseases, Shoot Bud Differentiation, Regeneration, Direct Organogenesis.
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  • Development of Triploid Plantlets from Immature Endosperm of Garcinia gummi-gutta var. gummigutta (L) Rob

Abstract Views: 193  |  PDF Views: 0

Authors

P. C. Rajendran
Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Kerala Agricultural University, Vellanikkara, Thrissur (Kerala), India
V. A. Rasmi
Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Kerala Agricultural University, Vellanikkara, Thrissur (Kerala), India
Heera Thomas
Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Kerala Agricultural University, Vellanikkara, Thrissur (Kerala), India

Abstract


The inoculated immature endosperm bits of 1cm size onto ½ MS supplemented with 3.0 mgl-1 each of BA and Kinetin induced adventitious buds and subsequent proliferation of multiple shoots. These triploid plantlets were ischolar_mained, hardened and planted out. Development of regeneration protocol from triploid parenchymatous tissue of endosperm not only overcome the dearth of demands for true-to-type propagules but also surpassed the gender dimorphism. Evolving seedlessness through in vitro endosperm culture may also be helpful to avoid the processing difficulty and to support the establishment of domestic HCA extraction unit for Women empowerment.

Keywords


Garcinia gummi-gutta Var. Gummigutta, Polygamodioecious, Kodampuli, Immature Seeds, Endosperm Culture, Triploid, Seedlessness, Life Style Diseases, Shoot Bud Differentiation, Regeneration, Direct Organogenesis.