Refine your search
Collections
Co-Authors
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Wali, Othman
- Gene Expression during Osteo/Odontogenic Differentiation of Mesenchymal Stem Cells with Platelet Rich Plasma and Mineral Trioxide Aggregate
Abstract Views :282 |
PDF Views:115
Authors
Amit Anandswarup Vanka
1,
Shanthi Vanka
2,
Sandeep Vishwakarma
3,
Manohar Bhat
4,
Dinesh Rao
5,
Othman Wali
6,
Aleem Khan
3
Affiliations
1 Pacific Academy of Higher Education and Research University, Udaipur − 313003, Rajasthan, IN
2 Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah − 22421, SA
3 Central Laboratory for Stem Cell Research and Translational Medicine, CLRD, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad − 500 058, Telangana, IN
4 Department of Pedodontics and Preventive Dentistry, Jaipur Dental College, Jaipur − 302015, Rajasthan, IN
5 Department of Pedodontics and Preventive Dentistry, Pacific Dental College and Hospital, Udaipur − 313011, Rajasthan, IN
6 Ibn Sina National College for Medical Studies, Jeddah − 22421, SA
1 Pacific Academy of Higher Education and Research University, Udaipur − 313003, Rajasthan, IN
2 Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah − 22421, SA
3 Central Laboratory for Stem Cell Research and Translational Medicine, CLRD, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad − 500 058, Telangana, IN
4 Department of Pedodontics and Preventive Dentistry, Jaipur Dental College, Jaipur − 302015, Rajasthan, IN
5 Department of Pedodontics and Preventive Dentistry, Pacific Dental College and Hospital, Udaipur − 313011, Rajasthan, IN
6 Ibn Sina National College for Medical Studies, Jeddah − 22421, SA
Source
Asian Journal of Pharmaceutical Research and Health Care, Vol 11, No 1 (2019), Pagination: 10-16Abstract
Platelet Rich Plasma (PRP) has the potential to regenerate pulp in immature pulpless teeth. Mineral trioxide is commonly used to seal the PRP into the pulp canal space. We investigated the effect of PRP and MTA individually and combined on osteo/odontogenic differentiation potential and the phenotype of tissue formed. MSCs were cultured in vitro with MTA, 5% PRP, 10% PRP, MTA with 5% PRP and MTA with 10% PRP. Osteo/odontogenic differentiation was assessed and quantified with alizarin red staining. Relative expression of Alkaline Phosphatase (ALP) activity, type 1 collagen (COL1A1), Dental Sialo-Phospho Protein (DSPP), Dentin Matrix Protein (DMP-1), Bone Gamma-carboxyglutamate Protein (BGLAP), Runt-related transcription factor 2 (Runx2), Osterix (Osx) and TGF-β1 was identified by RT-q PCR. 10% PRP with MTA displayed significantly higher calcium deposition during differentiation and high ALP levels. Significantly enhanced levels of DSPP, DMP1, COL1A1, BGLAP, Runx2, and Osx and TGF-β1 transcripts were observed. Within limitations of the in vitro environment, results imply enhanced osteodentin formation, on combining PRP with MTA.Keywords
Platelet Rich Plasma, Mineral Trioxide Aggregate, Mesenchymal Stem Cells, Odontogenic, Differentiation.Full Text
References
- Thomson A, Kahler B. Regenerative endodonticsbiologically-based treatment for immature permanent teeth: a case report and review of the literature. Aust Dent J. 2010; 55(4):446-52. https://doi.org/10.1111/j.18347819.2010.01268.x. PMid: 21133946.
- da Silva L, Nelson-Filho P, da Silva R, et al. Revascularization and periapical repair after endodontic treatment using apical negative pressure irrigation versus conventional irrigation plus triantibiotic intracanal dressing in dogs’ teeth with apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010; 109:779-87.
- Wang X, Thibodeau B, Trope M, Lin L, Huang G. Histologic characterization of regenerated tissues in canal space after the revitalization/revascularization procedure of immature dog teeth with apical periodontitis. J Endod. 2010; 36:5663.https://doi.org/10.1016/j.joen.2009.09.039. PMid: 20003936.
- Yamauchi N, Yamauchi S, Nagaoka H, et al. Tissue engineering strategies for immature teeth with apical periodontitis. J Endod. 2011; 37:390-97. https://doi.org/10.1016/j.joen.2010.11.010. PMid: 21329828.
- Mao JJ, Kim SG, Zhou J, Ye L, Cho S, Suzuki T, Fu SY, Yang R, Zhou X. Regenerative endodontics: Barriers and strategies for clinical translation. Dental Clinics. 2012 Jul 1; 56(3):639-49. https://doi.org/10.1016/j.cden.2012.05.005. PMid:22835543. PMCid: PMC4093795.
- Hwang YJ, Choi JY. Addition of mesenchymal stem cells to the scaffold of platelet-rich plasma is beneficial for the reduction of the consolidation period in mandibular distraction osteogenesis. J Oral Maxillofac Surg. 2010; 68:1112-24. https://doi.org/10.1016/j.joms.2008.08.038. PMid: 20223574.
- D’antò V, Di Caprio MP, Ametrano G, Simeone M, Rengo S, Spagnuolo G. Effect of mineral trioxide aggregate on mesenchymal stem cells. J Endod. 2010; 36:1839-43. https://doi.org/10.1016/j.joen.2010.08.010. PMid: 20951297.
- Vanka A, Sandeep KV, Shanthi G, Manohar KB, Wali O, Khan AA. Osteo/odontogenic differentiation of human mesenchymal stem cells with Platelet Rich Plasma (PRP) and Mineral Trioxide Aggregate (MTA). J Contemp Dent Pract (accepted for publication).
- Bausset O, Giraudo L, Veran J. Formulation and storage of platelet-rich plasma homemade product. Biores Open Access. 2012; 1:115-23. https://doi.org/10.1089/biores.2012.0225. PMid: 23516671, PMCid: PMC3559222.
- Vanka A, Sandeep KV, Shanthi G, Manohar KB, Khan AA. In vitro proliferation of MSCs using mineral trioxide aggregate: A most recent material for in situ stem cells mobilization. Int J of Adv Res. 2014; 2:561-67.
- Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem. 1987; 162:156-59. https://doi.org/10.1016/0003-2697(87)90021-2, https://doi.org/10.1006/abio.1987.9999. PMid: 2440339.
- Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg. 2004; 62:489-96. https://doi.org/10.1016/j.joms.2003.12.003. PMid: 15085519.
- Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: From Pure PlateletRich Plasma (P-PRP) to Leukocyte- and Platelet-Rich Fibrin (L-PRF). Trends Biotechnol. 2009; 27:158-67. https://doi.org/10.1016/j.tibtech.2008.11.009. PMid: 19187989.
- Paranjpe A, Smoot T, Zhang H, Johnson JD. Direct contact with mineral trioxide aggregate activates and differentiates human dental pulp cells. J Endod. 2011 Dec; 37(12):169-195. https://doi.org/10.1016/j.joen.2011.09.012. PMid: 22099907, PMCid: PMC3223385.
- Wang Y, Li J, Song W, Yu J. Mineral trioxide aggregate upregulates odonto/osteogenic capacity of bone marrow stromal cells from craniofacial bones via JNK and ERK MAPK signalling pathways. Cell proliferation. 2014 Jun; 47(3):241-48. https://doi.org/10.1111/cpr.12099. PMid: 24635197.
- Perut F, Filardo G, Mariani E, Cenacchi A, Pratelli L, Devescovi V, Kon E, Marcacci M, Facchini A, Baldini N, Granchi D. Preparation method and growth factor content of platelet concentrate influence the osteogenic differentiation of bone marrow stromal cells. Cytotherapy. 2013 Jul 1; 15(7):830-39. https://doi.org/10.1016/j.jcyt.2013.01.220. PMid: 23731763.
- Smith AJ, Scheven BA, Takahashi Y, Ferracane JL, Shelton RM, Cooper PR. Dentine as a bioactive extracellular matrix. Arch Oral Biol. 2012; 57:109-21. https://doi.org/10.1016/j.archoralbio.2011.07.008. PMid:21855856.
- Huang W, Yang S, Shao J, Li YP. Signaling and transcriptional regulation in osteoblast commitment and differentiation. Frontiers in Bioscience: A Journal and Virtual Library. 2007 May 1; 12:30-68. https://doi.org/10.2741/2296. PMid: 17485283, PMCid: PMC3571113.
- Linde A, Granstro¨ MG. Odontoblast alkaline phosphatases and Ca2þ transport. J Biol Buccale. 1978; 6:293-308.
- Couchourel D, Aubry I, Delalandre A. Altered mineralization of human osteoarthritic osteoblasts is attributable to abnormal type I collagen production. Arthritis and Rheumatology. 2009; 60:1438-50. https:// doi.org/10.1002/art.24489. PMid: 19404930, PMCid: PMC5250342.
- Miron RJ, Zhang YF. Osteoinduction: a review of old concepts with new standards. Journal of Dental Research. 2012 Aug; 91(8):736-44. https://doi.org/10.1177/0022034511435260. PMid:22318372.
- Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR, De Crombrugghe B. The novel zinc fingercontaining transcription factor osterix is required for osteoblast differentiation and bone formation. Cell. 2002 Jan 11; 108(1):17-29. https://doi.org/10.1016/S0092-8674(01)00622-5.
- Nakamura A, Dohi Y, Akahane M, Ohgushi H, Nakajima H, Funaoka H, Takakura Y. Osteocalcin secretion as an early marker of in vitro osteogenic differentiation of rat mesenchymal stem cells. Tissue Eng Part C Methods. 2009; 15:169-80. https://doi.org/10.1089/ten.tec.2007.0334. PMid: 19191495.
- Qin C, Brunn JC, Cadena E, Ridall A, Tsujigiwa H, Nagatsuka H, Nagai N, Butler WT. The expression of dentin sialophosphoprotein gene in bone. J Dent Res. 2002; 81:392-94. https://doi.org/10.1177/154405910208100607. PMid: 12097430.
- Massa LF, Ramachandran A, George A, Arana-Chavez VE. Developmental appearance of dentin matrix protein 1 during the early dentinogenesis in rat molars as identified by high-resolution immunocytochemistry. Histochem Cell Biol. 2005; 124:197-205. https://doi.org/10.1007/s00418-005-0009-9. PMid: 16049693.
- Teti G, Salvatore V, Ruggeri A, Manzoli L, GESI M, Orsini G, Falconi M. In vitro reparative dentin: A biochemical and morphological study. Eur J Histochem. 2013; 57:e23. https://doi.org/10.4081/ejh.2013.e23. PMid: 24085272, PMCid: PMC3794354.
- Bakopoulou A, Leyhausen G, Volk J, Tsiftsoglou A, Garefis P, Koidis P, Geurtsen W. Comparative analysis of in vitro osteo/odontogenic differentiation potential of human Dental Pulp Stem Cells (DPSCs) and Stem Cells from the Apical Papilla (SCAP). Arch Oral Biol. 2011; 56:709-21. https://doi.org/10.1016/j.archoralbio.2010.12.008. PMid: 21227403.
- Zhao L, Hantash BM. TGF-β1 regulates differentiation of bone marrow mesenchymal stem cells. Vitam Horm. 2011; 87:12741. https://doi.org/10.1016/B978-0-12-386015-6.00042-1. PMid: 22127241.
- Jadlowiec JA, Zhang X, Li J, Campbell PG and Sfeir C. Extracellular matrix-mediated signaling by dentin phosphophoryn involves activation of the Smad pathway independent of bone morphogenetic protein. The Journal of Biological Chemistry. 2006; 281(9):5341-47. https://doi.org/10.1074/jbc.M506158200. PMid: 16326713.
- Challenges in Medical Education on Student Learning, Teaching and Assessments
Abstract Views :262 |
PDF Views:121
Authors
Affiliations
1 Lecturer, Dentistry Program, Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah - 22421, SA
2 Associate Professor, Dentistry Program, Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah - 22421, SA
3 Vice-Dean, Dentistry Program, Department of Oral Basic and Clinical Sciences, Ibn Sina National College for Medical Studies, Jeddah - 22421, SA
1 Lecturer, Dentistry Program, Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah - 22421, SA
2 Associate Professor, Dentistry Program, Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah - 22421, SA
3 Vice-Dean, Dentistry Program, Department of Oral Basic and Clinical Sciences, Ibn Sina National College for Medical Studies, Jeddah - 22421, SA
Source
Asian Journal of Pharmaceutical Research and Health Care, Vol 12, No 2 (2020), Pagination: 82-84Abstract
Pandemic corona virus situation having progressed and become intensive by the end of academic year of so there is a big question in the minds of every teacher how to assess the students in medical institutes. The purpose of this review is to identify the various challenges in medical education the Universities are facing in learning, teaching and assessments and recommendations for coping strategies. The most pressing challenges that are emerging may include alternative assessments to the pen and paper assessments previously planned. In conclusion no matter what learning, teaching and assessment methods are employed by the various Universities each and every student has to have an individual responsibility to be self-accountable as they need to face the future with the knowledge they gain through these professional courses as online assessments are a significant technological advancement in today’s era.
Keywords
Assessments, COVID 19, Learning, Teaching.Full Text
References
- UNESCO. Exams and assessments in COVID-19 crisis: fairness at the centre. Accessed 18 April 2020 Available at https://en.unesco.org/news/exams-and-assessments-covid-19-crisis-fairness-centre.
- The Guardian. Medical students take final exams online for first time, despite student concern. Accessed 18 April 2020. Available at https://www.theguardian.com/education/2020/mar/22/coronavirus-forces-medical-students-sit-final-exams-online.
- Moodle. About Moodle. Accessed 19 April 2020. Available at https://docs.moodle.org/38/en/About_Moodle.
- UCL. Teaching and assessments during the coronavirus (COVID-19) outbreak. Accessed 19 April 2020. Available at https://www.ucl.ac.uk/students/exams-and-assessments/teaching-and-assessments-during-coronavirus-covid-19-outbreak.
- The World University Rankings. Asian universities face online assessment hurdles in virus crisis. Accessed 20 April 2020. Available at https://www.timeshighereducation.com/news/asian-universities-face-online-assessment-hurdles-virus-crisis.
- The World University Rankings. Educating despite the Covid-19 outbreak: lessons from Singapore. Accessed 20 April 2020. Available at https://www.timeshighereducation.com/blog/educating-despite-covid-19-outbreak-lessons-singapore# survey-answer.
- University of Essex. Alternative assessment during the COVID-19 outbreak. Accessed 20 April 2020. Available at https://www.essex.ac.uk/student/exams-and-coursework/alternative-assessment-during-the-covid-19-outbreak.
- UCL. Teaching and assessments during the coronavirus (COVID-19) outbreak. Accessed 20 April 2020. Available at https://www.ucl.ac.uk/students/exams-and-assessments/teaching-and-assessments-during-coronavirus-covid-19-outbreak.
- Knowledge Attitude and Practices of Patients Attending a Private Dental Hospital on Miswak
Abstract Views :217 |
PDF Views:101
Authors
Shanthi Vanka
1,
Renad Otaif
2,
Samar Salah Hassan
3,
Hanan Abdul Almajeed Alhashdi
3,
Eman Ali Alzahrani
4,
Othman Wali
5,
Amit Vanka
1,
Sajda Khan Gajdhar
1
Affiliations
1 Faculty of Dentistry, Dentistry Program, Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah, SA
2 Dentist, Beauty Secrets Clinics, Najran, SA
3 Dental Intern, Dentistry Program, Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah, SA
4 Dentist, Alnoor Specialist Hospital, Makkah, SA
5 Vice-Dean, Dentistry Program, Department of Oral Basic and Clinical Sciences, Ibn Sina National College for Medical Studies, Jeddah, SA
1 Faculty of Dentistry, Dentistry Program, Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah, SA
2 Dentist, Beauty Secrets Clinics, Najran, SA
3 Dental Intern, Dentistry Program, Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah, SA
4 Dentist, Alnoor Specialist Hospital, Makkah, SA
5 Vice-Dean, Dentistry Program, Department of Oral Basic and Clinical Sciences, Ibn Sina National College for Medical Studies, Jeddah, SA
Source
Asian Journal of Pharmaceutical Research and Health Care, Vol 13, No 4 (2021), Pagination: 282-287Abstract
Today, chewing sticks are being used commonly in the world. They are known by various names, including Miswak or arak in Arabic. The efficacy of Miswak and modern toothbrushes have shown a superior effect rather than use of toothbrush alone. This study aims to assess the Knowledge Attitude and Practices of patients attending a private dental hospital on Miswak. This is a cross-sectional analytical study. The study was conducted by means of a questionnaire, in order to obtain knowledge, attitude and practices of patients attending a private dental hospital on Miswak. An electronic copy of the questionnaire was prepared using Google forms in Arabic and distributed through social media. There were 342 respondents for the survey. About 93% of the participants selected that they use any oral hygiene aid as it is beneficial. Among all the participants only 2.3% of them used Miswak as an oral hygiene aid and 31.6% used both tooth brush and paste and Miswak. Majority of the respondents cleaned their teeth twice or thrice in a day. One of the main reasons of using Miswak by the participants was its religious background. The respondents prefer that the next generation would use a combination of Miswak and tooth brushKeywords
Attitude, Dental Clinics, Dental Patients, Knowledge, Miswak, Practices, ToothbrushFull Text
References
- Halawany HS. A review on Miswak (Salvadora Persica) and its effect on various aspects of oral health. Saudi Dent J. 1 Apr 2012; 24(2): 63–9. https://doi.org/10.1016/j.sdentj.2011.12.004
- Al Otaibi M, Al‐Harthy M, Gustafsson A, Johansson A, Claesson R, Angmar‐Månsson B. Subgingival plaque microbiota in Saudi Arabians after use of Miswak chewing stick and toothbrush. J Clin Periodontol. 31 Dec 2004 (12): 1048– 53. https://doi.org/10.1111/j.1600-051X.2004.00618.x
- Al-Hammadi AA, Al-Rabai NA, Togoo RA, Zakirulla M, Alshahrani I, Alshahrani A. Knowledge, attitude, and behavior related to use of Miswak (Chewing Stick): A cross-sectional study from aseer region, Saudi Arabia. Contemporary clinical dentistry. Jun 2018; 9(Suppl 1): S64. https://doi.org/10.4103/ccd.ccd_45_18
- Nordin A, Saim AB, Ramli R, Hamid AA, Nasri NW, Idrus RB. Miswak and oral health: An evidence-based review.Saudi J. Biol. Sci. 2020 1; 27(7): 1801–10. https://doi.org/10.1016/j.sjbs.2020.05.020
- Wu CD, Darout IA, Skaug N. Chewing sticks: timeless natural toothbrushes for oral cleansing. J Periodontal Rest of the examination is unchanged from previous evaluation. 2001; 36(5): 275–84. https://doi.org/10.1034/ j.1600-0765.2001.360502.x
- Dahiya P, Kamal R, Luthra RP, Mishra R, Saini G. Miswak: A periodontist’s perspective. J. Ayurveda Integr. Med. 2012; 3(4): 184. https://doi.org/10.4103/0975-9476.104431
- Patel PV, Shruthi S, Kumar S. Clinical effect of Miswak as an adjunct to tooth brushing on gingivitis. J Indian Soc Periodontol. 2012; 16: 84–8. https://doi.org/10.4103/0972124X.94611
- Al-Obaida MI, Al-Essa MA, Asiri AA, Al-Rahla AA. Effectiveness of a 20% Miswak extract against a mixture of Candida albicans and Enterococcus faecalis. Saudi Med J. 2010; 31: 640–3.
- Raosoft I. Sample size calculator by Raosoft, Inc.
- Bashir R, Rizvi K. Assessment of levels of oral hygiene awareness, knowledge, attitude and practice among the students of a government school in Karachi. Journal of Advances in Medicine and Medical Research. 2016 16: 1–1. https://doi.org/10.9734/BJMMR/2016/24794
- Alshahrani S, Alshuaibi A, Alkhaldi M, Koppolu P. Perception and Knowledge of Patients from Different Regions in the Kingdom of Saudi Arabia towards Oral Hygiene and Oral Hygiene Aids. In Healthcare 2021 May (Vol. 9, No. 5, p. 592). Multidisciplinary Digital Publishing Institute. https://doi.org/10.3390/healthcare9050592
- Haque MM, Alsareii SA. A review of the therapeutic effects of using Miswak (Salvadora Persica) on oral health. S Saudi Med. J. May 2015; 36(5): 530. https://doi.org/10.15537/smj.2015.5.10785
- World Health Organization. Prevention of oral diseases. World Health Organization; 1987.