Asian Journal of Pharmaceutical Research and Health Care

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Gene Expression during Osteo/Odontogenic Differentiation of Mesenchymal Stem Cells with Platelet Rich Plasma and Mineral Trioxide Aggregate


Affiliations
1 Pacific Academy of Higher Education and Research University, Udaipur − 313003, Rajasthan, India
2 Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah − 22421, Saudi Arabia
3 Central Laboratory for Stem Cell Research and Translational Medicine, CLRD, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad − 500 058, Telangana, India
4 Department of Pedodontics and Preventive Dentistry, Jaipur Dental College, Jaipur − 302015, Rajasthan, India
5 Department of Pedodontics and Preventive Dentistry, Pacific Dental College and Hospital, Udaipur − 313011, Rajasthan, India
6 Ibn Sina National College for Medical Studies, Jeddah − 22421, Saudi Arabia
 

Platelet Rich Plasma (PRP) has the potential to regenerate pulp in immature pulpless teeth. Mineral trioxide is commonly used to seal the PRP into the pulp canal space. We investigated the effect of PRP and MTA individually and combined on osteo/odontogenic differentiation potential and the phenotype of tissue formed. MSCs were cultured in vitro with MTA, 5% PRP, 10% PRP, MTA with 5% PRP and MTA with 10% PRP. Osteo/odontogenic differentiation was assessed and quantified with alizarin red staining. Relative expression of Alkaline Phosphatase (ALP) activity, type 1 collagen (COL1A1), Dental Sialo-Phospho Protein (DSPP), Dentin Matrix Protein (DMP-1), Bone Gamma-carboxyglutamate Protein (BGLAP), Runt-related transcription factor 2 (Runx2), Osterix (Osx) and TGF-β1 was identified by RT-q PCR. 10% PRP with MTA displayed significantly higher calcium deposition during differentiation and high ALP levels. Significantly enhanced levels of DSPP, DMP1, COL1A1, BGLAP, Runx2, and Osx and TGF-β1 transcripts were observed. Within limitations of the in vitro environment, results imply enhanced osteodentin formation, on combining PRP with MTA.

Keywords

Platelet Rich Plasma, Mineral Trioxide Aggregate, Mesenchymal Stem Cells, Odontogenic, Differentiation.
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  • Gene Expression during Osteo/Odontogenic Differentiation of Mesenchymal Stem Cells with Platelet Rich Plasma and Mineral Trioxide Aggregate

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Authors

Amit Anandswarup Vanka
Pacific Academy of Higher Education and Research University, Udaipur − 313003, Rajasthan, India
Shanthi Vanka
Department of Preventive Dental Sciences, Ibn Sina National College for Medical Studies, Jeddah − 22421, Saudi Arabia
Sandeep Vishwakarma
Central Laboratory for Stem Cell Research and Translational Medicine, CLRD, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad − 500 058, Telangana, India
Manohar Bhat
Department of Pedodontics and Preventive Dentistry, Jaipur Dental College, Jaipur − 302015, Rajasthan, India
Dinesh Rao
Department of Pedodontics and Preventive Dentistry, Pacific Dental College and Hospital, Udaipur − 313011, Rajasthan, India
Othman Wali
Ibn Sina National College for Medical Studies, Jeddah − 22421, Saudi Arabia
Aleem Khan
Central Laboratory for Stem Cell Research and Translational Medicine, CLRD, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad − 500 058, Telangana, India

Abstract


Platelet Rich Plasma (PRP) has the potential to regenerate pulp in immature pulpless teeth. Mineral trioxide is commonly used to seal the PRP into the pulp canal space. We investigated the effect of PRP and MTA individually and combined on osteo/odontogenic differentiation potential and the phenotype of tissue formed. MSCs were cultured in vitro with MTA, 5% PRP, 10% PRP, MTA with 5% PRP and MTA with 10% PRP. Osteo/odontogenic differentiation was assessed and quantified with alizarin red staining. Relative expression of Alkaline Phosphatase (ALP) activity, type 1 collagen (COL1A1), Dental Sialo-Phospho Protein (DSPP), Dentin Matrix Protein (DMP-1), Bone Gamma-carboxyglutamate Protein (BGLAP), Runt-related transcription factor 2 (Runx2), Osterix (Osx) and TGF-β1 was identified by RT-q PCR. 10% PRP with MTA displayed significantly higher calcium deposition during differentiation and high ALP levels. Significantly enhanced levels of DSPP, DMP1, COL1A1, BGLAP, Runx2, and Osx and TGF-β1 transcripts were observed. Within limitations of the in vitro environment, results imply enhanced osteodentin formation, on combining PRP with MTA.

Keywords


Platelet Rich Plasma, Mineral Trioxide Aggregate, Mesenchymal Stem Cells, Odontogenic, Differentiation.

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DOI: https://doi.org/10.18311/ajprhc%2F2019%2F24218