Journal of Natural Remedies

Open Access Open Access  Restricted Access Subscription Access

Treatment of Thyponium Flageliforme in Combination with Curcuma Zedoaria, and Phyllanthus Niruri Synergistically Enhances Apoptotic and Anti-Proliferative Effect on Breast Cancer


Affiliations
1 Department of Anatomy and Master Program of Biomedical Sciences of Sultan Agung Islamic University, Semarang, Indonesia
2 Department of Chemistry, Sultan Agung Islamic University, Semarang, Indonesia
3 Department of Biochemistry, and Master Program of Biomedical Sciences of Sultan Agung Islamic University, Semarang, Indonesia
 

Introduction: Breast cancer is the most common cancer and the second leading cause of cancer death in women. Combinations of Thyponium flageliforme, Curcuma zedoaria and Phyllanthus niruri (TCP) have been proven as immunomodulator and retarded tumor progression in vivo. Objective: to investigate the apoptotic and anti-proliferative effect of TCP in C3H mice suffered from adenocarcinoma cells. Methods: 18 mice inoculated with one million cells of breast adenocarcinoma per mouse were assigned into three groups, TCP with dosage 85 and 125 mg were treated for 21 days to evaluate tumor volume, expression of Ki-67 and Capase3. Expression of Ki-67 and Capase3 were measured by immunohistochemistry staining, whilst cancer volume was assessed using calipers. Results: Mann Whitney analysis indicated that tumor volume in TCP groups were lower, otherwise for expression of caspase3, p < 0.05 respectively. Whereas expression of Ki-67 in TCP groups was lower, but not significant, p > 0.05. Conclusion: TCP treatment with 85 and 125 mg dosage capable of reducing tumor volume, increasing caspase3 and lowering Ki-67 expression.

Keywords

Apoptosis, Breast Cancer, Thyponium flageliforme, Tumor Volume.
Font Size

User

Notifications
JOURNAL COVERS
  

  • Jnr FNG, Anyanful A, Eliason S, Adamu SM, Debrah S.Pattern of breast cancer distribution in ghana: a survey to enhance early detection, diagnosis, and treatment.International Journal of Breast Cancer. 2016; 1–9.

  • Youlden DR, Cramb SM, Dunn NA, Muller JM, Pyke CM, Baade PD. The descriptive epidemiology of female breast cancer: An international comparison of screening, incidence, survival and mortality. Cancer Epidemiol. 2012; 36:237–48. PMid:22459198. https://doi.org/10.1016/j.canep.2012.02.007

  • Bombonati A, Sgroi DC. The molecular pathology of breast cancer progression. J Pathol. 2011; 223: 307–17.PMid:21125683 PMCid:PMC3069504. https://doi.org/10.1002/path.2808

  • Youlden DR, Cramb SM, Yip CH, Baade PD. Incidence and mortality of female breast cancer in the Asia-Pacific region. Cancer Biol Med. 2014; 11:101–15. PMid:25009752 PMCid:PMC4069805

  • American Cancer Society. Cancer Facts and Figures 2016.Atlanta, GA: American Cancer Society; 2016.

  • Lakshmi L, Padmaja G, and Remani P. Antitumor effects of isocurcumenol isolated from curcuma zedoaria rhizomes on human and murine cancer cells.International Journal of Medicinal Chemistry. 2011; 1–13. PMid:27429805 PMCid:PMC4939266. https://doi.org/10.1155/2011/253962

  • Tang YQ, Lee SH, Sekaran SD. Phyllanthus Sp a local plant with multiple medicinal properties. JUMMEC. 2014; 17:1–8.

  • Tang Y-Q, Jaganath IB, Sekaran SD. Phyllanthus spp. induces selective growth inhibition of PC-3 and MeWo human cancer cells through modulation of cell cycle and induction of Apoptosis. PLoS ONE. 2010; 5:e12644. PMid:20838625 PMCid:PMC2935893. https://doi.org/10.1371/journal.pone.0012644

  • Lee SH, Jaganath, Wang SM, Sekaran S D. Antimetastatic effect of phyllanthus in human lung (A 549) and breast (MCF-7) cancer cell lines. Journal PLos one. 2011; e20994.PMid:21698198 PMCid:PMC3116853. https://doi.org/10.1371/journal.pone.0020994

  • Meiyanto E, Hamid IS. Modulasi cyp1a1 dan gst serta ekspresi p53 dan ras setelah induksi 7,12-dimethyl benz(ά) antrasen (dmba) dan pemberian anti karsinogenesis gynura procumbens dan curcuma zedoaria pada tikus galur sprague dawley. J. Penelit Med Eksakta. 2009; 8:168– 77.

  • Mohan S, Abdul AB, Abdalwahab SI, Al-Zubairi AS, Sukari MA, Abdullah R, et al. Typhonium flagelliforme inhibits the proliferation of murine leukemia WEHI-3 cells invitro and induces apoptosis invivo. Leuk Res. 2010; 34:1483–92. PMid:20569984. https://doi.org/10.1016/j.leukres.2010.04.023

  • Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. The Scientific World Journal.2013; 1–16. PMid:24327805 PMCid:PMC3845396. https:// doi.org/10.1155/2013/962185

  • Mahomoodally MF, Gurib-Fakim A, Subratty AH. Antimicrobial activities and phytochemical profiles of endemic medicinal plants of Mauritius.Pharmaceutical Biology. 2005; 43:237–42.https://doi.org/10.1080/13880200590928825

  • Yamamoto Y, Gaynor RB. Therapeutic potential of inhibition of the NF-κB pathway in the treatment of inflammation and cancer. The Journal of Clinical Investigation. 2001; 107:135–42. PMid:11160126 PMCid:PMC199180. https:// doi.org/10.1172/JCI11914

  • Sousa CR, Miranda-Vilela AL, de Almeida MC, Fernandes JMS1, Sebben A, Magalhaes KG, et al. Breast cancer model developed through orthotopic Ehrlich cell injection for investigation of mechanisms in malignancy and metastasis; 2015.

  • Gregorio AC, Fonseca NA, Moura V, Lacerda M, Figueiredo P, Simoes S, et al. Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model, PLoS ONE. 2016; e0165817 11:1–19.

  • Neudert M, Fischer C, Krempien B, Bauss F, Seibel Mj. Sitespecific human breast cancer (Mda-Mb-231) metastases in nude rats: model characterisation and in vivo effects of ibandronate on tumour growth. Int J Cancer. 2003; 107:468–77. PMid:14506749. https://doi.org/10.1002/ ijc.11397

  • Marsden CG, Mary Jo Wright MJ, Carrier L, Moroz K, Pochampally R, Rowan BG. A novel in vivo model for the study of human breast cancer metastasis using primary breast tumor-initiating cells from patient biopsies. BMC Cancer. 2012; 12:1–16. PMid:22233382 PMCid:PMC3277457. https://doi.org/10.1186/14712407-12-10

  • Resende FA, Vilegas W, dos Santos LC, Varanda EA. Mutagenicity of flavonoids assayed by bacterial reverse mutation (ames) test. Molecules. 2012; 17:5255–68. PMid:22565478. https://doi.org/10.3390/ molecules17032335

  • Nasihun T, Widayati E. Administration of purwoceng (Pimpinellaalpina Molk) improves oxidative stress biomarker following UVC irradiationin Spargue-Dawley male rats. Journal of Natural Remedies. 2016; 15:82–91.

  • Porter AG, Janicke RU. Emerging roles of caspase-3 in apoptosis. Cell Death and Differentiation. 1999; 6:99–104.

  • PMid:10200555. https://doi.org/10.1038/sj.cdd.4400476

  • Rahman ASM, Wahab AN, Malek ASN. In vitro morphological assessment of apoptosis induced by antiproliferative constituents from the rhizomes of Curcuma zedoaria. 2013.

  • Shin Y, Lee Y. Cytotoxic activity from curcuma zedoaria though mitochondrial activation on ovarian cancer cells. Toxicol Res. 2013; 29:257–61. PMid:24578796 PMCid:PMC3936178. https://doi.org/10.5487/ TR.2013.29.4.257

  • Mankaran S, Dinesh K, Deepak S, Gurmeet. Typhonium flagelliforme: A multipurpose plant. Int Res J Pharm.2013; 4:45–8. https://doi.org/10.7897/2230-8407.04308

  • Fuida S, Debatin KM. Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene. 2006; 25:4798–811. PMid:16892092. https://doi.org/10.1038/ sj.onc.1209608

  • Chen CC, Chen Y, His YT, Chang CS, Huang LF, Ho CT, et al. Chemical constituents and anticancer activity of Curcuma zedoaria Roscoe essential oil against non-small cell lung carcinoma cells in vitro and in vivo. J Agric Food. 2013; 61:11418–27. PMid:24199734. https://doi.org/10.1021/jf4026184


Abstract Views: 320

PDF Views: 174




  • Treatment of Thyponium Flageliforme in Combination with Curcuma Zedoaria, and Phyllanthus Niruri Synergistically Enhances Apoptotic and Anti-Proliferative Effect on Breast Cancer

Abstract Views: 320  |  PDF Views: 174

Authors

Chodijah
Department of Anatomy and Master Program of Biomedical Sciences of Sultan Agung Islamic University, Semarang, Indonesia
Eni Widayati
Department of Chemistry, Sultan Agung Islamic University, Semarang, Indonesia
Taufiqurrachman Nasihun
Department of Biochemistry, and Master Program of Biomedical Sciences of Sultan Agung Islamic University, Semarang, Indonesia

Abstract


Introduction: Breast cancer is the most common cancer and the second leading cause of cancer death in women. Combinations of Thyponium flageliforme, Curcuma zedoaria and Phyllanthus niruri (TCP) have been proven as immunomodulator and retarded tumor progression in vivo. Objective: to investigate the apoptotic and anti-proliferative effect of TCP in C3H mice suffered from adenocarcinoma cells. Methods: 18 mice inoculated with one million cells of breast adenocarcinoma per mouse were assigned into three groups, TCP with dosage 85 and 125 mg were treated for 21 days to evaluate tumor volume, expression of Ki-67 and Capase3. Expression of Ki-67 and Capase3 were measured by immunohistochemistry staining, whilst cancer volume was assessed using calipers. Results: Mann Whitney analysis indicated that tumor volume in TCP groups were lower, otherwise for expression of caspase3, p < 0.05 respectively. Whereas expression of Ki-67 in TCP groups was lower, but not significant, p > 0.05. Conclusion: TCP treatment with 85 and 125 mg dosage capable of reducing tumor volume, increasing caspase3 and lowering Ki-67 expression.

Keywords


Apoptosis, Breast Cancer, Thyponium flageliforme, Tumor Volume.

References





DOI: https://doi.org/10.18311/jnr%2F0%2F8448