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Zafarullah, S. N.
- Saliva Signature of Normal Pregnant Women in each Trimester as Analyzed by FTIR Spectroscopy
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Authors
Affiliations
1 Department of Physics, Justice Basheer Ahmed Sayeed College for Women, Chennai-600018
2 Department of Physics, Easwari Engineering College, Chennai 600089, IN
3 Obstetrics & Gyneacology, Stanley Medical College & Govt. RSRM Lying. In Hospital, Chennai-600013, IN
1 Department of Physics, Justice Basheer Ahmed Sayeed College for Women, Chennai-600018
2 Department of Physics, Easwari Engineering College, Chennai 600089, IN
3 Obstetrics & Gyneacology, Stanley Medical College & Govt. RSRM Lying. In Hospital, Chennai-600013, IN
Source
Indian Journal of Science and Technology, Vol 4, No 5 (2011), Pagination: 481-486Abstract
We report the changes in salivary pattern of normal pregnant women in each trimester qualitatively and quantitatively. FTIR spectroscopic technique has been successfully applied to analyze the saliva samples of various age groups from18 to 25 and 25 to 35. The role of FTIR spectroscopy in the analysis of saliva of normal pregnant women of 1st, 2nd and 3rd trimester of various age groups are clearly demonstrated both qualitatively and quantitatively. Among the three trimesters, the vibrational peaks of the progesterone increased in the 1st trimester: whereas, in the 2nd trimester there was a decrease: In 3rd trimester there was a further decrease. Similarly, in the case of normal pregnant women of age between 25 to 35 has a greater absorption value than the normal pregnant women of age 18 to 25. This may be attributed to the salivary IgA and IgM concentrations higher in ageing people. It is concluded that FTIR salivary signature may be applied to indicate different stage of pregnancy of different age group.Keywords
Saliva, Normal Pregnant Women, Trimester, FTIR Spectroscopic Analysis.Full Text
References
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- Insulin Response of Diabetic Pregnant Women: Analysis of Saliva by FTIR Study
Abstract Views :456 |
PDF Views:113
Authors
Affiliations
1 Deptartment of Physics, Justice Basheer Ahmed Sayeed College for Women, Chennai-600018, IN
2 Department of Physics, Easwari Engineering College, Chennai 600089, IN
3 Obstetrics & Gyneacology, Stanley Medical College & Superintendent i/c, Govt. RSRM Lying in Hospital, Chennai- 600013, IN
1 Deptartment of Physics, Justice Basheer Ahmed Sayeed College for Women, Chennai-600018, IN
2 Department of Physics, Easwari Engineering College, Chennai 600089, IN
3 Obstetrics & Gyneacology, Stanley Medical College & Superintendent i/c, Govt. RSRM Lying in Hospital, Chennai- 600013, IN
Source
Indian Journal of Science and Technology, Vol 5, No 3 (2012), Pagination: 2318-2324Abstract
This paper presents the FTIR spectral study of saliva of normal and diseased (diabetes) and its use in differentiating those pregnant women on insulin therapy. FTIR saliva spectra were recorded over the region 4000-400 cm-1 on a Spectrum one Perkin Elmer FTIR spectrometer. Saliva samples were collected from 20 volunteers in each set of age group 25 to 35. From the spectral study, the absorbance values of the diseased and normal saliva samples were compared. The spectral differences of intensity ratio parameters were introduced and the results were discussed. In order to find the efficacy, the absorption values of the specific bands of the spectra of normal and diabetic pregnant women were compared and the internal ratio parameter R1( I1653/I1410), R2(I2931/I547), R3(I2931/I1410), R4(I2931/I3292),R5(I3292/I547) were calculated , and the result observed for diabetic pregnant women after insulin therapy (i.e., 3 hrs and 7 days) was almost similar to that of the normal pregnant women. Besides, the intensity of absorption peaks of the diabetic pregnant women before administration of insulin, after intake of insulin (3hrs) and after insulin therapy (7 days) were considered in which the intensity ratio parameters R1( I1544/I1075), R2 (I1544/I1224), R3(I2931/I1075), R4(I3292/I1075), R5(I3292/I1224), were calculated. The observed absorbance values increased in diabetic pregnant women initially, but slightly decreased after insulin therapy 3 hrs and further decreased after 7 days compared to normal pregnant women. Thus, striking spectral differences (in terms of intensity value) observed between saliva of normal, diseased subjects when analysed after insulin therapy. The comparison of these values showed that the spectra of normal and diabetic pregnant women before and after therapy are different and the vibrational analyses were carried out. It is concluded that FTIR may be applied to indicate the changes in the salivary pattern of the diabetic pregnant women with insulin therapy.Keywords
Saliva, Normal Pregnant Women, Diabetic Pregnant Women, Insulin, FTIR Spectroscopic AnalysisFull Text
References
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- Utility of FTIR Spectroscopic Analysis of Saliva of Diabetic Pregnant Women in each Trimester
Abstract Views :461 |
PDF Views:116
Authors
Affiliations
1 Deptartment of Physics, Justice Basheer Ahmed Sayeed College for Women, Chennai-600018, IN
2 Department of Physics, Easwari Engineering College, Chennai 600089, IN
3 Obstetrics & Gyneacology, Stanley Medical College & Superintendent i/c, Govt. RSRM Lying in Hospital, Chennai- 600013, IN
1 Deptartment of Physics, Justice Basheer Ahmed Sayeed College for Women, Chennai-600018, IN
2 Department of Physics, Easwari Engineering College, Chennai 600089, IN
3 Obstetrics & Gyneacology, Stanley Medical College & Superintendent i/c, Govt. RSRM Lying in Hospital, Chennai- 600013, IN
Source
Indian Journal of Science and Technology, Vol 4, No 8 (2011), Pagination: 967-970Abstract
FTIR spectroscopy has been successfully employed to compare the saliva of the diseased and normal pregnant women. The absorption values of the specific bands of the spectra in each trimester compared, and the internal ratio parameter are calculated. The study shows that there is a change in the salivary pattern of normal and diseased women in each trimester.Keywords
Saliva, Normal Pregnant Women, Diabetic Pregnant Women, Trimester, FTIR Spectroscopic AnalysisFull Text
References
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- Dovbeshko GI, Girdina NY, Kruglova EB and Pashchuk OP (1997) FTIR Spectroscopy studies of nucleic acid damage. Talanta. 53, 233-246.
- Dovbeshko GI, Chegel VI, Gridina NY, Repnytska OP, Shirshov YM,Tryndiak VP, Todor IM and Solyanik GI (2002) Surface enhanced IR absorption of nucleic acids from tumor cells: FTIR reflectance study. Biopolymer (Biospectroscopy). 67, 470–486.
- Fabian H. Jackson M, Murphy L, Watson PH, Fichtner I and Mantsch HH (1995) A comparative infrared spectroscopic study of human breast tumors and breast tumor cell xenografts. Biospectroscopy. 1 (1), 37–45.
- Fujioka N, Morimoto Y, Arai T and Kikuchi M (2004) Discrimination between normal and malignant human gastric tissues by Fourier transform infrared spectroscopy. Cancer Detection & Prevention. 28, 32– 36.
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- Raziya Sultana R, Zafarullah SN and Hephzibah Kirubamani N (2011) Saliva signature of normal pregnant women in each trimester (ISSN 0974-6846) Indian J. Sci. Technol. 4 (5),481-486.
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- Analysis of Saliva and Serum of Normal and Anomalies Pregnant Women - Folic Acid Deficiency using FTIR Spectroscopy
Abstract Views :229 |
PDF Views:0
Authors
Affiliations
1 Deptartment of Physics, Justice Basheer Ahmed Sayeed College for Women, Chennai-600018, IN
2 Department of Physics, Easwari Engineering College, Chennai-600089, IN
3 Saveetha Medical College, Saveetha Nagar Thandalam, Irrungattukottai, Kanchipuram–602105, IN
1 Deptartment of Physics, Justice Basheer Ahmed Sayeed College for Women, Chennai-600018, IN
2 Department of Physics, Easwari Engineering College, Chennai-600089, IN
3 Saveetha Medical College, Saveetha Nagar Thandalam, Irrungattukottai, Kanchipuram–602105, IN
Source
Indian Journal of Science and Technology, Vol 7, No 3 (2014), Pagination: 367–373Abstract
This study is an attempt to evaluate and compare the spectral difference in saliva and serum between healthy pregnant women and anomalies pregnant women due to folic acid deficiency, using Fourier Transform Infrared Spectroscopy. Folic acid is required for the development of a healthy fetus and plays an important role in the development of the fetus spinal cord and brain. The present work is to study the folic acid deficiency in pregnancy-Anomalies (open neural defect) and compare the results with the normal healthy pregnant women. The results showed that there is a significant difference between the folic acid of healthy pregnant and anomalies (open neural defect) in pregnant women both in saliva and serum sample. From the spectral study, the intensity ratio parameters R1 (I1338/I3415) R2 (I3546/I1636) R3 (I2854/I3415) R4(I1743/I3546) R5 (I1482/I1511) R6 (I1482/I1696) R7 (I1135/I1607) R8 (I2927/I1607) R9 (I2927/I1604) R10 (I1492/I1511) have been introduced and computed. The outcome of the results shows that FTIR can be used in the qualitative and quantitative investigation of biological fluids to distinguish sample sets from healthy and diseased groups. The internal standard method is adopted in characterizing the samples quantitatively.Keywords
Folic Acid Deficiency, FTIR, Pregnant Women, SalivaFull Text