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Sharma, Veena
- Ameliorative Effects of Operculina turpethum and its Isolated Stigma‑5,22dien‑3-o-b-D-glucopyranoside on the Hematological Parameters of Male Mice Exposed to N‑Nitrosodimethylamine, a Potent Carcinogen
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Toxicology International (Formerly Indian Journal of Toxicology), Vol 21, No 1 (2014), Pagination: 29-36Abstract
Objectives: Enormous propensity of plants to synthesize a variety of structurally diverse bioactive compounds, has made the plant kingdom a potential source of chemical constituents with various therapeutic values, including antitumor and cytotoxic activities. Blood is a good indicator to determine the physiological and pathological status of man and animal. The objective of the present study is to determine the effect of Operculina turpethum ischolar_main extract and its isolated glycoside treatment on the hematological parameters in the mice with N‑Nitrosodimethylamine (NDMA) induced cancer. Materials and Methods: The body weights of the animals were recorded before and after the experiment. Non‑coagulated blood was tested for total erythrocyte count, total leukocyte count, hemoglobin, differential leukocyte count (DLC) and for other blood indices. Results: A significant (P < 0.01), (P < 0.001) recovery of the red blood cell and white blood cell counts, packed cell volume and hemoglobin content in the host after 21 day treatment was shown. Conclusion: These results show that the extract of Operculina turpethum is relatively safe following oral administration and have possible stimulatory effect on red blood cell production and there was dose dependent therapeutic effect.Keywords
Blood, haematology, N‑Nitrosodimethylamine, Operculina turpethum- Protective Assessment of Euphorbia neriifolia and its Isolated Flavonoid Against N‑nitrosodiethylamine‑induced Hepatic Carcinogenesis in Male Mice: A Histopathological Analysis
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Toxicology International (Formerly Indian Journal of Toxicology), Vol 21, No 1 (2014), Pagination: 37-43Abstract
Aims: The aim of this study was to examine the impacts of N‑nitrosodiethylamine (DENA), a potent environment carcinogen on liver tissue of mice which was attenuated by isolated flavonoid and hydro‑ethanolic extract of Euphorbia neriifolia (HEEN) leaves. Materials and Methods: Carcinogenicity was induced in albino mice by a single oral administration of DENA (50 mg/kg body weight). The HEEN (150 and 400 mg/kg body weight), butylated hydroxyanisole (BHA; 0.5 and 1%) and E. neriifolia flavonoid (ENF; 50 mg/kg body weight) were estimated to examine the possible anti‑cancer potential. Results: DENA exposed animals showed alterations in normal hepatic histo‑architecture, which comprised of necrosis (N), dilated sinusoids and vacuolization of the cells. Mice treated with E. neriifolia lower (ENL) and higher (ENH) dose and ENF before intoxicated with DENA showed that the liver cells were normal, with very little necrosis (Day 31). On the other hand, BHA higher (BHAH) and lower (BHAL) dose failed to diminish the abnormalities caused by the DENA. Conclusion: Results of the present study suggests that the ENH and ENF protects the hepatic tissue against DENA‑induced hepatic carcinoma. The results could also be expressed in the order of ENH> ENF> ENL> BHAH> BHAL.Keywords
Euphorbia neriifolia, flavonoid, liver, necrosis, N‑nitrosodiethylamine- Chemopreventive Role of Euphorbia neriifolia (Linn) and its Isolated Flavonoid Against N-Nitrosodiethylamine-induced Renal Histopathological Damage in Male Mice
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Toxicology International (Formerly Indian Journal of Toxicology), Vol 20, No 1 (2013), Pagination: 101-107Abstract
Aims: This study is an attempt to evaluate the tissue protective efficacy of isolated flavonoid and hydro‑ethanolic extract of Euphorbia neriifolia (HEEN) leaves against N‑nitrosodiethylamine (DENA) induced renal carcinoma. Materials and Methods: Carcinogenicity was induced in Albino mice by oral administration of DENA (50 mg/kg body weight). The HEEN (150 and 400 mg/kg body weight), Butylated hydroxyanisole (BHA; 0.5 and 1%), and Euphorbia neriifolia flavonoid (ENF; 50 mg/kg body weight) were evaluated for their possible tissue carcinogenesis protective potential. Results: DENA treated animals showed alterations in normal renal histo‑architecture, which comprised of necrosis (N) and vacuolization of the cells. On the other hand, the mice treated with Euphorbia neriifolia lower (ENL) and higher (ENH) dose and ENF before intoxicated with DENA showed that the renal cells were normal (Day 31). Whereas, BHA higher (BHAH) and lower (BHAL) dose failed to diminish the abnormalities caused by DENA. Conclusions: The findings of the present study Suggested that ENH and ENF showed highest renal-protective activity among all the pretreatments. The results could also be expressed in the order of ENH > ENF > ENL > BHAH > BHAL.Keywords
Euphorbia neriifolia, flavonoid, kidney, necrosis, N‑nitrosodiethylamine- Ameliorative Effects of Tinospora Cordifolia Root Extract on Histopathological and Biochemical Changes Induced by Aflatoxin-B1 in Mice Kidney
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Authors
Rekha Gupta
1,
Veena Sharma
1
Affiliations
1 Department of Bioscience and Biotechnology, Banasthali University, Banasthali-304022, Rajasthan, IN
1 Department of Bioscience and Biotechnology, Banasthali University, Banasthali-304022, Rajasthan, IN
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Toxicology International (Formerly Indian Journal of Toxicology), Vol 18, No 2 (2011), Pagination: 94-98Abstract
The present study was planned to investigate the ability of the Tinospora cordifolia to scavenge free radicals generated during aflatoxicosis. A total no. of 48 male Swiss albino mice (30 ± 5 g) were exposed to Aflatoxin B1 (AFB1) (2 μg/30 g b.wt, orally) either individually or in combination with T. cordifolia (50, 100, 200 mg/kg, orally) once daily for 25 days. AFB1 exposure led to significant rise in thiobarbituric acid reactive substances (TBARS) and fall in superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR), ascorbic acid, and protein content. T. cordifolia was found to show protective effect by lowering down the content of TBARS and enhancing the GSH, ascorbic acid, protein, and the activities of antioxidant enzymes viz., SOD, CAT, glutathione peroxidase, GST, and GR in kidney. Histopathological analysis of kidney samples also confirmed the protective values and antioxidant activity of ethanolic extract of herb. T. cordifolia showed protection against aflatoxin-induced nephrotoxicity due to the presence of alkaloids such as a choline, tinosporin, isocolumbin, palmatine, tetrahydropalmatine, and magnoflorine.Keywords
Aflatoxin, antioxidant, mice, oxidative stress, Tinospora cordifolia- Anti-hepatotoxic Potential of Indigofera tinctoria and its isolated Isothiocyanate compound ‘ITC-1’ against NPYR-CCl4 Intoxicated Mice
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Authors
Rashmi Singh
1,
Veena Sharma
2
Affiliations
1 Department of Biotechnology and Microbiology, Alpine Group of Management and Technology, Dehradun – 248001, Uttarakhand, IN
2 Department of Bioscience and Biotechnology, Banasthali University, Vanasthali – 304022, Rajasthan, IN
1 Department of Biotechnology and Microbiology, Alpine Group of Management and Technology, Dehradun – 248001, Uttarakhand, IN
2 Department of Bioscience and Biotechnology, Banasthali University, Vanasthali – 304022, Rajasthan, IN
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Toxicology International (Formerly Indian Journal of Toxicology), Vol 26, No 1&2 (2019), Pagination: 30-36Abstract
Isothiocyanate derivative from hydroethanolic extract of Indigofera tinctoria (HEIT) was previously isolated, purified and characterized as 1-[1,2-Diisothiocyanato-2-(3-isothiocyanato-2,2-dimethyl-propylsulfanyl)-ethoxy]-3-isothiocyanato-2,2-dimethyl-propane (C16H22N4OS5; m/z 446.70; ITC-1). To elucidate hepatoprotective efficacy, liver toxicity was induced in mice via intoxication of N-Nitrosopyrrolidine (NPYR) followed by CCl4 for 50 days. Both low and high doses of crude HEIT were given orally to NPYR treated mice for 21 days. Silymarin was also administered to compare the results. After completion of post treatment, various hepatic toxicity markers were evaluated. Results showed that both doses of HEIT and ITC-1 have successfully normalized the levels of AST, ALT (P<0.001 v/s NPYR treated group), ALP and bilirubin (P<0.01). ITC-1 has showed better remedial response against liver toxicity in comparison to Silymarin. Thus, we concluded that both I. tinctoria and ‘ITC-1’ have future remedial aspect in diminution of hepatic toxicity.Keywords
AST, Bilirubin, Hepatic Toxicity, Indigofera tinctoria, Isothiocyanate Compounds, Silymarin.References
- Abdallah H, Mohamed M, Abdou A, Hamed M, AbdelNaim A, Ashour O. Protective effect of Centaurea pallescens Del. against CCl4-induced injury on a human hepatoma cell line (Huh7). Med Chem Res. 2013; 22:5700–6. https:// doi.org/10.1007/s00044-013-0563-y
- Srivastava A, Shivanandappa T. Hepatoprotective effect of the ischolar_main extract of Decalepishamiltonii against carbon tetrachlorideinduced oxidative stress in rats. Food Chem. 2010; 118:411–7. https://doi.org/10.1016/j.foodchem.2009.05.014
- Kerry JP, Kerry JF. Processed meats: Improving safety, nutrition and quality. Sinko P, editor. Heat and processing generated contaminants in processed meats. Cambridge: Woodhead Publishing Ltd. 2011. https://doi.org/10.1533/9780857092946
- Hecht SS, Upadhyaya P, Wang M. Evolution of research on the DNA Adduct Chemistry of N-Nitrosopyrrolidine and Related Aldehydes. Chem ResToxicol. 2011; 24:781–90. PMid: 21480629 PMCid: PMC3118975. https://doi.org/10.1021/tx200064a
- Nadkarni KM.I ndian Materia medica. Bombay: Popular Prakashan; 1996.
- Savithramma N, Sulochana Ch, Rao KN. Ethnobotanical survey of plants used to treat asthma in Andhra Pradesh, India. J Ethnopharmacol. 2007; 113:4–61. PMid: 17606346. https://doi.org/10.1016/j.jep.2007.04.004
- Motamarri SN, Arthikeyan M, Rajsekar S, Gopal V. Indigofera tinctoria Linn - A phytopharmacological review. Int J Res Pharm BiomedSci. 2012; 3:164–9.
- Kris-Etherton PM, Hecker HD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, et al. Bioactive compounds in foods: Their role in the prevention of cardiovascular disease and cancer. Am J Med. 2002; 113:71S–88S. https://doi.org/10.1016/S0002-9343(01)00995-0
- Khandelwal KP. Practical Pharmacology: Techniques and experiments. Pune: Nirali Prakashan; 2006.
- Sharma V, Singh R. Isolation and structural elucidation of an isothiocyanate compound from Indigoferatinctoria Linn. extract. Curr Sci. 2017; 113:941–6. https://doi.org/10.18520/cs/v113/i05/941-946
- Singh BN, Singh BR, Sharma BK, Singh HB. Potential chemoprevention of N-nitrosodiethylamine-induced hepatocarcinogenesis by polyphenolics from Acacia nilotica bark. Chem Biol Interact. 2009; 181:20–8. PMid: 19446540. https://doi.org/10.1016/j.cbi.2009.05.007
- Priyadarsini G, Kumar A, Anbu J, Anjana A, Ayyaswamy A. Nephroprotective activity of decoction of Indigofera tinctoria (Avurikudineer) against cisplatin-induced nephropathy in rats. Int J Life Sci Pharm Res. 2012; 2:56–62.
- Tian CC, Zha XQ, Luo JP. A polysaccharide from Dendrobium huoshanensehepatic inflammatory response caused by carbon tetra chloride. Biotechnol Biotechnol Equip 2015; 29:132–8. PMid: 26019626 PMCid: PMC4434038. https://doi.org/10.1080/13102818.2014.987514
- Reitman S, Frankel S. A method of assaying liver enzymes in human serum. Am J Clin Pathol. 1957; 28:56–8. PMid: 13458125. https://doi.org/10.1093/ajcp/28.1.56
- Sadashivam S, Manickam A. Phenolics: Biochemical Methods. New Delhi: New Age International Publishers; 2004.
- Floyd RA, Soong LM, Stuart MA, Reigh DL. Spin trapping of free radicals produced from nitrosamine carcinogens. Photochem Photobiol. 1978; 28:857–62. PMid: 216035. https://doi.org/10.1111/j.1751-1097.1978.tb07032.x
- Rao GM, Rao Ch.V, Pushpangadan P, Annie S. Hepatoprotective effects of rubiadin, a major constituent of Rubia Cordifolia Linn. J Ethnopharmacol. 2006; 103:484–90. PMid: 16213120. https://doi.org/10.1016/j.jep.2005.08.073
- Rej R. Measurement of aminotransferases: Part 1. Aspartate aminotransferases. CRC Crit Rev Clin Lab Sci. 1984; 21:99–106. PMid: 6391817. https://doi.org/10.3109/10408368409167137
- Dufour DR, Lott JA, Nolte FS, Gretch DR, Koff RS, Seeff LB. Diagnosis and monitoring of Hepatic injury: Recommendations for use of Laboratory tests in screening, diagnosis and monitoring. Clin Chem. 2000; 46:2050–68.
- Pol S, Nalpas B, Vassault A, Bousquet-Lemercier B, Franco D, Lacour B, et al. Hepatic activity and mRNA expression of aspartate aminotransferase isoenzymes in alcoholic and non-alcoholic liver disease. Hepatology 1991; 14:620–5. https://doi.org/10.1002/hep.1840140408
- Jahan R, Vani G, Shyamaladevi CS. Anticarcinogenic effect of Solanum trilobatum in Diethylnitrosamine induced and Phenobarbital promoted hepatocarcinogenesis in rats. Asian J Biochem. 2011; 6:74–81. https://doi.org/10.3923/ajb.2011.74.81
- Sahu CR. Mechanisms involved in toxicity of liver caused by Piroxicam in mice and protective effects of leaf extracts of Hibiscus rosa-sinensis L. Clin Med Insights Artheritis Musculoskelet Disord. 2016; 9:9–13. PMid: 26819562 PMCid: PMC4720181. https://doi.org/10.4137/CMAMD.S29463
- Singh R, Kumar S, Rana AC, Sharma N. Different models of Hepatotoxicity and related liver disease: A review. Int Res J Pharma. 2012; 3:86–95.
- MLAB2401- Clinical Chemistry Lab Manual CF113. UNIT: Total and Direct Bilirubin. www.2ndchance.info/dxme-BilirubinUrine-defDirect.pdf
- Singh R, Sharma S, Sharma V. Comparative and quantitative analysis of antioxidant and scavenging potential of Indigofera tinctoria Linn. extracts. J Integr Med. 2015; 13:269–78. https://doi.org/10.1016/S2095-4964(15)60183-2
- Immunomodulating Effects of Allium sativum in Renal Tissue of LPS Challenged Mice
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1 Department of Bioscience and Biotechnology, Banasthali Vidyapith, Vanasthali Road - 304022, Rajasthan, IN
1 Department of Bioscience and Biotechnology, Banasthali Vidyapith, Vanasthali Road - 304022, Rajasthan, IN
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Toxicology International (Formerly Indian Journal of Toxicology), Vol 26, No 3&4 (2019), Pagination: 80–88Abstract
Allium sativum contain various medicinal properties like immunomodulatory, antitumorigenetic, antiatherosclerotic and antimicrobial. In the present study, we analyzed the impact of hydroethanol extract of Allium sativum on cytokine production in LPS challenged mice. LPS significantly induced the activation of NF-kB and also increased the levels of NO, TNF-α, IL-6, IFN-Y, PGE2 with the suppression of IL-10 production. In the results we found that Allium sativum significantly attenuated the LPS-induced phosphorylation of NF-ĸB and normalized the levels of NO, TNF-α, IL-6, IFN-Y, PGE-2 and IL-10. In summary, it was deduced that garlic may promote an anti-inflammatory effect by inhibition of NF-kB activity in the tissue.Keywords
Allium sativum, Anti-inflammatory, Cytokines, Lipopolysaccharide (LPS), NF-kB.References
- Kabanov DS, Vwedenskaya OY, Fokina MA, Morozova EM, Grachev SV, Prokhorenko IR. Impact of CD14 on reactive oxygen species production from human leukocytes primed by Escherichia coli lipopolysaccharides. Oxidative Medicine and Cellular Longevity. 2019. PMid: 30944694 PMCid: PMC6421816. https://doi.org/10.1155/2019/6043245
- Gholamnezhad Z, Hassanabad ZF. Effects of lipopolysaccharide-induced septic shock on rat isolated kidney, possible role of nitric oxide and protein kinase C pathways. Iranian Journal of Basic Medical Sciences. 2018 Oct; 21(10):1073.
- Minasyan H. Sepsis: Mechanisms of bacterial injury to the patient. Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine. 2019 Dec; 27(1):19. PMid: 30764843 PMCid: PMC6376788. https://doi.org/10.1186/s13049019-0596-4
- Imo C, Za’aku JS. Medicinal properties of ginger and garlic: A review. Current Trends of Biomedical Engineering and Biosciences. 2019; 18:2.
- Puccinelli M, Stan S. Dietary bioactive diallyl trisulfide in cancer prevention and treatment. International Journal of Molecular Sciences. 2017 Aug; 18(8):1645. PMid: 28788092 PMCid: PMC5578035. https://doi.org/10.3390/ijms18081645
- Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite and [15N] nitrate in biological fluids. Analytical Biochemistry. 1982 Oct; 126(1):131–8. https://doi.org/10.1016/0003-2697(82)90118-X
- Mc Manus FA, Mowry RW. Staining methods: In histology and histochemistry. (P.B. Hoeber Ed). New York: Harper and Brothers; 1965.
- Patel NS, Chatterjee PK, Di Paola R, Mazzon E, Britti D, De Sarro A, Cuzzocrea S, Thiemermann C. Endogenous interleukin-6 enhances the renal injury, dysfunction and inflammation caused by ischemia/reperfusion. J Pharmacol Exp Ther. 2005; 312:1170–8. PMid: 15572648. https://doi.org/10.1124/jpet.104.078659
- Shin JH, Ryu JH, Kang MJ, Hwang CR, Han J, Kang D. Shortterm heating reduces the anti-inflammatory effects of fresh raw garlic extracts on the LPS-induced production of NO and pro-inflammatory cytokines by downregulating allicin activity in RAW 264.7 macrophages. Food and Chemical Toxicology. 2013 Aug; 58:545–51. PMid: 23583806. https://doi.org/10.1016/j.fct.2013.04.002
- Xiao ZY, Zheng QY, Jiang YY, Zhou B, Yin M, Wang HB, Zhang JP. Effects of esculentoside A on production of interleukin-1, 2 and prostaglandin E~ 2. Acta Pharmacologica Sinica. 2004 Jun; 25(6):817-21.
- Marquez Martin A, De La Puerta Vazquez R, FernandezArche A, Ruiz-Gutierrez V. Supressive effect of maslinic acid from pomace olive oil on oxidative stress and cytokine production in stimulated murine macrophages. Free Radical Research. 2006 Jan; 40(3):295–302. PMid: 16484046. https://doi.org/10.1080/10715760500467935
- Heba G, Krzemiński T, Porc M, Grzyb J, Ratajska A, Dembinska-Kiec A. The time course of tumor necrosis factor-α, inducible nitric oxide synthase and vascular endothelial growth factor expression in an experimental model of chronic myocardial infarction in rats. Journal of Vascular Research. 2001; 38(3):288–300. PMid: 11399901. https://doi.org/10.1159/000051057
- Salvemini D, Ischiropoulos H, Cuzzocrea S. Roles of nitric oxide and superoxide in inflammation. InInflammation Protocols. 2003. p. 291–303). Humana Press. PMid: 12769497. https://doi.org/10.1385/1-59259-374-7:291
- Funk CD, Funk LB, Kennedy ME, Pong AS, Fitzgerald GA. Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression and gene chromosomal assignment. The FASEB Journal. 1991 Jun; 5(9):2304–12. PMid: 1907252. https://doi.org/10.1096/fasebj.5.9.1907252
- Subbaramaiah K, Dannenberg AJ. Cyclooxygenase 2: A molecular target for cancer prevention and treatment. Trends in Pharmacological Sciences. 2003 Feb; 24(2):96–102. https://doi.org/10.1016/S0165-6147(02)00043-3
- Gradolatto A, Canivenc-Lavier MC, Basly JP, Siess MH, Teyssier C. Metabolism of apigenin by rat liver phase I and phase II enzymes and by isolated perfused rat liver. Drug Metabolism and Disposition. 2004 Jan; 32(1):58–65. PMid: 14709621. https://doi.org/10.1124/dmd.32.1.58
- Freeman F, Kodera Y. Garlic chemistry: Stability of S-(2propenyl)-2-propene-1-sulfinothioate (allicin) in blood, solvents and simulated physiological fluids. Journal of Agricultural and Food Chemistry. 1995 Sep; 43(9):2332–8. https://doi.org/10.1021/jf00057a004
- Jeong HJ, Koo HN, Na HJ, Kim MS, Hong SH, Eom JW, Kim KS, Shin TY, Kim HM. Inhibition of TNF-α and IL-6 production by aucubin through blockade of NF-κB activation in RBL-2H3 mast cells. Cytokine. 2002 Jun; 18(5):252–9. PMid: 12161100. https://doi.org/10.1006/cyto.2002.0894
- Kim SW, Choi SC, Choi EY, Kim KS, Oh JM, Lee HJ, Oh HM, Kim S, Oh BS, Kimm KC, Lee MH. Catalposide, a compound isolated from Catalpa ovata, attenuates induction of intestinal epithelial proinflammatory gene expression and reduces the severity of trinitrobenzene sulfonic acid-induced colitis in mice. Inflammatory Bowel Diseases. 2004 Sep; 10(5):564–72. PMid: 15472516. https://doi.org/10.1097/00054725200409000-00010
- Faggioni R, Fantuzzi G, Fuller J, Dinarello CA, Feingold KR, Grunfeld C. IL-1β mediates leptin induction during inflammation. Am J Physiol Regul Integr Comp Physiol. 1998; 274:R204–8. PMid: 9458919. https://doi.org/10.1152/ajpregu.1998.274.1.R204
- Sharma A, Sharma V, Kansal L. Amelioration of leadinduced hepatotoxicity by Allium sativum extracts in Swiss albino mice. Libyan Journal of Medicine. 2010 Jan; 5(1):4621. PMid: 28156294. https://doi.org/10.3402/ljm.v5i0.4621
- Isolation, Characterization and Elucidation of Invigorative Potential of Flavonoid from Stem-bark of Prosopis Cineraria on Lps-induced Oxidative Stress and Inflammatory Cascade in Swiss Albino Male Mice
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1 Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali – 304022, Rajasthan, IN
1 Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali – 304022, Rajasthan, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 27, No 3&4 (2020), Pagination: 136-148Abstract
The present research aimed to elucidate the structure and characterize the isolated compound from stem-bark of Prosopis cineraria and unravel its potential against LPS-induced toxicity in mouse model. The spectral techniques were done for characterization and structure elucidation of the isolated compound (HPLC, NMR, FT-IR, LC-MS. The experimental mice were intoxicated (intra-peritoneal) with LPS (2 mg/kg body weight) and further treated with isolated compound from Prosopis cineraria (15 mg/kg body weight). Dexamethasone was used as a standard (10 mg/kg body weight). The oxidative stress parameters (LPO, CAT, SOD, GSH, GST and GPx) and biochemical activities (AST, ALT, ACP and ALP) were studied. The levels of pro-inflammatory cytokines (TNF-α; Prostaglandins E2; IL-6; NF-κBp65; IFN-γ and IL-10) were determined in liver homogenate. Nitric Oxide (NO) produced due to LPS-intoxication was determined by using Griess reagent. The results of the spectral analysis were used to elucidate the structure of the isolated flavonoid. The isolated flavonoid suppressed the over-expression and altered levels of oxidative parameters and cytokines due to LPS intoxication and restored the levels of TNF-α, NF-κB, NO, IL-6, IFN- , Prostaglandin E2 and IL-10. The research investigation unfolded the alleviating potential of the isolated compound against LPS-induced adverse effects by modulating the expression of cytokines and combating oxidative stress.Keywords
Anti-inflammatory, Cytokines, Flavonoids, Oxidative Stress, Prosopis cineraria, ROSReferences
- Singer M, Deutschman CS, Seymour CW. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA. 2016; 315(8):801–10. PMid:26903338PMCid:PMC4968574. https://doi. org/10. 1001/jama.2016.0287
- Callery MP, Kamei T, Flye MW. Kupffer cell blockade increases mortality during intra-abdominal sepsis despite improving systemic immunity. Arch Surg. 1990;125(1):36–41.PMid:2294881. https://doi. org/10.1001/archsurg.1990.01410130038005
- Nostro A, Germano MP, D’angelo V, Marino A, Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett.o Appl.o Microbiol. 2000; 30:379. PMid: 10792667. https://doi.org/10.1046/j.1472-765x.2000.00731.x
- Nandkarni KM. Indian Materia Medica. Vol. 1. Popular Prakashan: Mumbai; 2000.
- Mohammad IS, Muhammad H, Shoaib Khan NA, Rasool F. Biological potential and phytochemical evaluation of Prosopis cineraria. World Appl Sci J. 2013; 27:1489–94.
- Sumathi S, Dharani B, Sivaprabha J. Cell death induced by methanolic extract of Prosopis cineraria leaves in MCF-7 breast cancer cell line. Int J Pharmacol Sci Invent. 2013; 2:21–6.
- Nwanjo HU, Ojiako OA. Effect of vitamins E and C on exercise induced oxidative stress. Global J Pure Appl Sci. 2005; 12(2):199–202. https://doi.org/10.4314/gjpas.v12i2.16591
- Marklund S, Marklund G. Involvement of superoxide anion radical in the auto-oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47(3):469–74. PMid: 4215654. https:// doi.org/10.1111/j.1432-1033.1974.tb03714.x
- Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105:121–6. https://doi.org/10.1016/S0076-6879(84) 05016-3
- Reitman S, Frankel AS. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. American J Clin Pathol. 1957; 28:53–6. PMid: 13458125. https://doi.org/10.1093/ ajcp/28.1.56
- Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82:70–7. https://doi.org/10.1016/0003- 9861(59)90090-6
- Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 249(22):7130–9.
- Rotruck JT, Pope AL, Ganther HE. Selenium: Biochemical role as a component of Glutathione Peroxidases. Science. 1973; 179(4073):588–90. PMid: 4686466. https://doi. org/10.1126/science.179.4073.588
- Lowry OH, Rosenbrough AL, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem. 1951; 193:265–75.
- Sadashivam S, Manickam A. Biochemical methods. Vol 2. New Delhi, India: New Age International (P) Limited; 1996.
- Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite,and (15N) nitrate in biological fluids. Anal Biochem. 1982;126:131–8. https://doi.org/10.1016/0003-2697(82) 90118-X
- Esterbauer H, Dieber-Rotheneder M, Waeg G, Striegl G, Juergens G. Biochemical structural and functional properties of oxidized low-density lipoprotein. Chem Res Toxicol. 1990; 3(2):77–92. PMid: 2130945. https:// doi.org/10.1021/tx00014a001
- Kangralkar VA, Patil SD, Bandivadekar RM. Oxidative stress and diabetes: A review. Intl J Pharm Appl. 2010; 1:38–45.
- Yasui K, Baba A. Therapeutic potential of Superoxide Dismutase (SOD) for resolution of inflammation. Inflamm Res. 2006; 55:359–63. PMid: 17122956. https:// doi.org/10.1007/s00011-006-5195-y
- Bowler RP, Nicks M, Tran K, Tanner G, Chang LY, Young SK. Extracellular superoxide dismutase attenuates lipopolysaccharide induced neutrophilic inflammation. Am J Respir Cell Mol Biol. 2004; 31:432–9. PMid: 15256385. https://doi.org/10.1165/rcmb.2004-0057OC
- Joseph A, Li Y, Koo HC, Davis JM, Pollack S, Kazzaz JA. Superoxide dismutase attenuates hyperoxia-induced interleukin-8 induction via AP-1. Free Radic Biol Med. 2008; 45:1143–9. PMid: 18692129. https://doi. org/10.1016/j.freeradbiomed.2008.07.006
- Porfire AS, Leucuţa SE, Kiss B, Loghin F, Pârvu AE. Investigation into the role of Cu/Zn-SOD delivery system on its antioxidant and anti-inflammatory activity in rat model of peritonitis. Pharmacol Rep. 2014; 66:670–6. PMid: 24948070. https://doi.org/10.1016/j. pharep.2014.03.011
- Perez-Rivero G, Ruiz-Torres MP, Diez-Marques ML, Canela A, Lopez-Novoa JM, Rodriguez-Puyol M. Telomerase deficiency promotes oxidative stress by reducing catalase activity. Free Radic Biol Med. 2008; 45:1243–51. PMid: 18718525. https://doi.org/10.1016/j. freeradbiomed.2008.07.017
- Ito K, Nakazato T, Yamato K, Miyakawa Y, Yamada T, Hozumi N. Induction of apoptosis in leukemic cells by homovanillic acid derivative, capsaicin, through oxidative stress: Implication of phosphorylation of p53 at Ser-15 residue by reactive oxygen species. Cancer Res. 2004; 64:1071–8. PMid: 14871840. https://doi. org/10.1158/0008-5472.CAN-03-1670
- Yu L, Wan F, Dutta S, Welsh S, Liu Z, Freundt E. Autophagic programmed cell death by selective catalase degradation. Proc Natl Acad Sci USA, 2006; 103:4952–7. PMid: 16547133 PMCid: PMC1458776. https://doi.org/10.1073/pnas.0511288103
- Abdalla MY. Glutathione as potential target for cancer therapy; more or less is good? (mini-review). Jordan J Biol Sci. 2011; 4(3):119–24.
- Nada SA, El-Shamarka ME-S, Omara EA, Abdel-Salam OM. Grape seed extract and Vitamin C combination blocked LPS-induced multiple organ toxicity in mice. ROS. 2019; 7(21):161–75. https://doi.org/10.20455/ ros.2019.827
- Jahan MS, Vani G, Shyamaladevi CS. Anti-carcinogenic effect of solanum trilobatum in diethylnitrosamine induced and Phenobarbital promoted hepatocarcinogenesis in rats. Asian J Biochem. 2011; 6(1):74–81. https://doi.org/10.3923/ajb.2011.74.81
- Han YJ, Kwon YG, Chung HT. Antioxidant enzymes suppress Nitric Oxide production through the inhibition of NF-kappa B activation: role of H2O2 and Nitric Oxide in inducible Nitric Oxide synthase expression in macrophages. Nitric Oxide. 2001; 5(5):504–13. PMid: 11587565.
- RH Shih, CY Wang, CM Yang. NF-kappaB signaling pathways in neurological inflammation: A mini review. Front. Mol Neurosci, 2015; 8:77. PMid: 26733801 PMCid:PMC4683208. https://doi.org/10.3389/fnmol. 2015.00077
- Choi YY, Kim MH, Hong J, Kim SH, Yang WM. Dried ginger (Zingiber officinalis) inhibits inflammation in alipopolysaccharide- inducedmousemodel.Evid-based Complementary Altern Med. 2013; 914563. PMid: 23935687 PMCid: PMC371222 https://doi. org/10.1155/2013/914563
- Park HS, Jung HY, Park EY, Kim J, Lee WJ, Bae YS. Cutting edge: Direct interaction of TLR4 with NAD(P) H oxidase 4 isozyme is essential for lipopolysaccharide- induced production of reactive oxygen species and activation of NF-kappa B. J Immunol. 2004; 173(6):3589–93.PMid: 15356101. https://doi. org/10.4049/jimmunol.173.6.3589
- Erridge C, Bennett-Guerrero E, Poxton IR. Structure and function of lipopolysaccharides. Microbes Infect. 2002; 4(8):837–51. https://doi.org/10.1016/ S1286-4579(02)01604-0
- Kirtikar KR, Basu BD. Indian medicinal plants Vol. 2. Dehradun India: International Book Distributors; 1984.
- Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agric Food Chem. 1998; 46:41137. https://doi.org/10.1021/jf9801973
- Khatri A, Rathore A, Patil UK. Assessment of anti- inflammatory activity of bark of Prosopis cineraria (L.) Druce. Int J Pharm Res. 2012; 4(2):27–9.
- Sachdeva S, Kaushik V, Saini V. A review on phytochemical and pharmacological potential of Prosopis cineraria. Int J Ethnobiol Ethnomed. 2014; 1(1):1–4.
- Rotelli AE, Guardia T, Juarez AO, de la Rocha NE, Pelzer LE. Comparative study of flavonoids in experimental models of inflammation. Pharmacol Res. 2003; 48:601–6. https://doi.org/10.1016/S1043-6618(03)00225-1
- Galvez J, de la Cruz JP, Zarzuelo A, Sanchez de Medina FJ, Jimenez J Sanchez, de la Cuesta F. Oral administration of quercitrin modifies intestinal oxidative status in rats. Gen Pharmacol. 1994; 25:1237– 43. https://doi.org/10.1016/0306-3623(94)90143-0
- Comalada M, Camuesco D, Sierra S, Ballester I, Xaus J, Galvez J, Zarzuelo A. In vivo quercetin anti- inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-kappaB pathway. Eur J Immunol. 2005; 35:584–92. PMid: 15668926. https://doi.org/10.1002/eji.200425778
- Sawatzky D, Willoughby D, Colville-Nash P, Rossi A. The involvement of the apoptosis-modulating proteins Erk 1/2, Bcl-xL and Bax in the resolution of acute inflammation in vivo. Am J Pathol. 2006; 168:33–41. PMid: 16400007 PMCid: PMC1592663. https://doi. org/10.2353/ajpath.2006.050058
- Validating the Antimicrobial Potentiality of Peptides from Pods of Acacia nilotica Willd.ex Delile: A Spotlight on Bacterial Fauna
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Authors
Richa Shukla
1,
Veena Sharma
1
Affiliations
1 Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk - 304022, Rajasthan, IN
1 Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk - 304022, Rajasthan, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 30, No 1 (2023), Pagination: 111-119Abstract
Contagious infections cause over 17 million human deaths in a year among which diseases caused by bacteria are the most prominent ones. Additionally, in the last few years, the haphazard usage of antibiotics has provoked the condition of multifarious defiance in the bacterial strains against these chemical drugs thus contributing to an upsurge towards severe economic and communal welfare-related uncertainties. Thus, to annihilate such situations there is a compelling demand to explore novel substitutes to overcome these concerns. Hence, such problematic situations bring up the limelight towards plant-derived proteins/peptides possessing antimicrobial activity which prove to be excellent alternatives against bacterial pathogens causing serious maladies in humans. Therefore, in this context the current study investigated the antibacterial potential of the Ethanol, Methanol and Chloroform peptides/protein isolate obtained from pods of Acacia nilotica by Disc-Diffusion Assay and MIC (Minimum Inhibitory Concentration) against E. coli and B. subtilis bacterial strains. The antibacterial activity of all three solvent peptide isolates was found to be exhibiting antimicrobial activity at a concentration of 100μg/ml against B. subtilis with Disc-Diffusion as well as MIC with Chloroform isolate displaying the highest activity against gram-positive Bacillus species. Therefore, such plant-derived antibacterial proteins/peptides in the future can be used as a therapeutic drug to combat various severe bacterial maladies thus providing a better alternative than antibiotics both in terms of safety against unwanted secondary effects as well as will also help in bringing down the graphs of Antimicrobial Resistance (AMR) posing a serious threat to the human health. As a result, the study concludes that peptide isolates obtained from Acacia nilotica pods are effective against gram-positive Bacillus species, making them an important antibacterial compound.Keywords
Acacia nilotica, Antimicrobial Resistance, Plant-Derived Antimicrobial Peptides.References
- Datta S, Roy A. Antimicrobial peptides as potential therapeutic agents: A review. Int J of Peptide Research and Therapeutics. 2021; 27:555-577. https://doi. org/10.1007/s10989-020-10110-x
- Vouga M, Greub G. Emerging bacterial pathogens: Past and Beyond. Clinical Microbiology and Infection. 2015; 22:12-21. https://doi.org/10.1016/j.cmi.2015.10.010 PMid:26493844 PMCid:PMC7128729
- Vogelmann R, Amieva MR. The role of bacterial pathogens in cancer. Current Opinion in Microbiology. 2007; 10:76-81. https://doi.org/10.1016/j.mib.2006.12.004 PMid:17208515
- Farsimadan M, Motamedifar M. Bacterial infection of the male reproductive system causing infertility. Journal of Reproductive Immunology. 2020; 142. https://doi. org/10.1016/j.jri.2020.103183 PMid:32853846
- Prakash D, Saxena RS. Distribution and antimicrobial susceptibility pattern of bacterial pathogens causing urinary tract infection in urban community of Meerut city, India. ISRN Microbiol. 2013; 29. https:// doi.org/10.1155/2013/749629 PMid:24288649 PMCid:PMC3830820
- Raghunath D. Emerging antibiotic resistance in bacteria with special reference to India. J Biosci. 2008; 33:593- 603. https://doi.org/10.1007/s12038-008-0077-9 PMid:19208984
- Sang Y, Blecha F. Antimicrobial peptides and bacteriocins: alternatives to traditional antibiotics. Anim Health Res Rev. 2008; 9:227-35. https://doi.org/10.1017/ S1466252308001497 PMid:18983725
- Moretta A, Scieuzo C, Petrone AM, Salvia R, Manniello MD, Franco A, Lucchetti D, Vassallo A, Vogel H, Sgambato A, Falabella P. Antimicrobial peptides: A new hope in biomedical and pharmaceutical fields. Front Cell Infect Microbiol. 2021; 11. https://doi. org/10.3389/fcimb.2021.668632 PMid:34195099 PMCid:PMC8238046
- Rana D. A review of ethnomedicine, phytochemical and pharmacological properties of Acacia nilotica (Babool/Kikkar). Int J of Bio Pharmacy and Allied Sciences. 2018; 7:856-863. https://doi.org/10.31032/ IJBPAS/2018/7.5.4443
- Abdalla MS, Babiker IA , Al-Abrahim JS, Mohammed AE, Elobeid MM, Elkhalifa KF. Fodder potential and chemical composition of Acacia nilotica fruits for livestock in the dry lands of Sudan. Int J of Plant, Animal and Environmental Sciences. 2014; 4:366-369.
- Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45:493-496. https://doi. org/10.1093/ajcp/45.4_ts.493 PMid:5325707
- Pandian CJ, Palanivel R, Dhanasekaran S. Screening antimicrobial activity of nickel nanoparticles synthesized using Ocimum sanctum leaf extract. J of Nanoparticles. 2016; 2016:1-13. https://doi.org/10.1155/2016/4694367
- Salas CE, Badillo-Corona JA, Ramírez-Sotelo G, Oliver- Salvador C. Biologically active and antimicrobial peptides from plants. Biomed Res Int. 2015; 2015. https://doi.org/10.1155/2015/102129 PMid:25815307 PMCid:PMC4359881
- Benko-Iseppon AM, Galdino SL, Calsa T Jr, Kido EA, Tossi A, Belarmino LC, Crovella S. Overview on plant antimicrobial peptides. Curr Protein Pept Sci. 2010; 11:181-8. https://doi.org/10.2174/138920310791112075 PMid:20088772
- de Souza Cândido E, e Silva Cardoso MH, Sousa DA, Viana JC, de Oliveira-Júnior NG, Miranda V, Franco OL. The use of versatile plant antimicrobial peptides in agribusiness and human health. Peptides. 2014; 55:65- 78. https://doi.org/10.1016/j.peptides.2014.02.003 PMid:24548568
- Goyal RK, Mattoo AK. Multitasking antimicrobial peptides in plant development and host defense against biotic/abiotic stress. Plant Sci. 2014; 228:135-49. https:// doi.org/10.1016/j.plantsci.2014.05.012 PMid:25438794
- Gruenheid S, Le Moual H. Resistance to antimicrobial peptides in Gram-negative bacteria. FEMS Microbiol Lett. 2012; 330:81-9. https://doi.org/10.1111/j.1574- 6968.2012.02528.x PMid:22339775