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Farahin Ehlam, Siti Nur
- Cytotoxicity and Genotoxicity Assessments of Batik Industrial Wastewater on V79 Cells
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Authors
Affiliations
1 Environmental Health and Industrial Safety Programme, School of Diagnostic and Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, MY
1 Environmental Health and Industrial Safety Programme, School of Diagnostic and Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, MY
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 22, No 3 (2015), Pagination: 76-82Abstract
Batik is well-known in Malaysian textile industry. It is one of the rapidly growing industries inherited from a generation to another. The manufacturer of batik industry usually discharges the wastewater containing hazardous pollutants (a mixture of chemicals especially reactive dyes, waxes, alum, resin, and sodium silicate) into the environment without any preliminary treatment. This study was conducted to assess the cytotoxicity and genotoxicity of batik industrial wastewater in three drums of wastewater on V79 cells as a preliminary study for a toxicity testing. The physicochemical properties of the wastewater were assessed. The cytotoxicity was assessed by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Meanwhile, the genotoxicity of the wastewater was evaluated via alkaline Comet assay. This study found that the wastewater from all the three drums demonstrated a cytotoxic effect toward the V79 cells at various concentrations, and drum A showed lower IC50 value compared to drum B and C. The IC50 in drum A are 8.8%, 8.0%, and 8.4% v/v for December 2014, January 2015, and February 2015, respectively. Meanwhile, for genotoxicity study of the wastewater samples on V79 cells, the study found that the value of tail moment for all samples were lower than 2 scores with the highest score is 1.842±0.150, while the results for the negative and positive controls were 6.5±1.079 and 0.436±0.012, respectively. In conclusion, the wastewater from all three the drums in this study had a cytotoxic effect but did not demonstrate a genotoxic effect on V79 cells, indicating no DNA damage inflicted.Keywords
Batik Wastewater, Cytotoxicity, Genotoxicity, V79 Cells.
References
- Hai FI, Yamamoto K, Fukushi K. Development of a submerged membrane fungi reactor for textile wastewater treatment. Desalination. 2006; 192:315–22.
- Babu BR, Parande A, Raghu S. and Kumar TP Textile technology: Cotton textile processing: Waste generation and effluent treatment. Journal of Cotton Science. 2007; 11–141.
- Banat IM, Nigam P, Singh D, Marchant R. Microbial decolorization of textile-dyecontaining effluents: A review. Bioresource Technology. 1996; 58:217–27.
- Noor S, Rohasliney H. A preliminary study on batik effluent in kelantan state: A water quality perspective. International Conference on Chemical, Biological and Environment Sciences, Bangkok; 2011. p. 274–6.
- Sridewi N, Tan LT, Sudesh K. Solar photocatalytic decolorization and detoxification of industrial batik dye wastewater using P (3HB)-TiO2 nanocomposite films. Clean. 2011; 39:265–73.
- Siti ZM, Nurhaslina C, Halim KK. Influence of agitation, pH and temperature on growth and decolorization of batik wastewater by bacteria Lactobacillus delbruckii. IRJES. 2013; 14:2–5.
- Rashidi HR, Sulaiman NM, Hashim NA. Batik industry synthetic wastewater treatment using nanofiltration membrane. Procedia Eng. 2012; 44:2010–2.
- Rashidi H, Sulaiman NN, Hashim N, Hassan C. The application of hybrid physical pretreatment system for treatment of simulated batik. Wastewater. 2012; 55:1–2015.
- Wahid ZA, Munaim MS. Substainable technology for treatment of batik waste effluent. Google Patents; 2011.
- Ahmad AL, Harris WA, Ooi BS. Removal of dye from wastewater of textile industry using membrane technology. Journal of Technology. 2012; 36:31–44.
- Anjaneyulu Y, Chary NS, Raj DS. Decolourization of industrial effluents- Available methods and emerging technologiesA review. Rev Environ Sci Biotechnol. 2005; 4:245–73.
- Puvaneswari N, Muthukrishnan J, Gunasekaran P. Toxicity assessment and microbial degradation of azo dyes. Indian J Exp Biol. 2006; 44:618–26.
- Hach. Spectrophotometer procedure manual. USA: Hach Company; 2002.
- Zegura B, Heath E, Cernosa A, Filipic M. Combination of in vitro bioassays for the determination of cytotoxic and genotoxic potential of wastewater, surface water and drinking water samples. Chemosphere. 2009; 75:1453–60.
- Chaung W, Mi LJ, Boorstein RJ. The p53 status of Chinese hamster V79 cells frequently used for studies on DNA damage and DNA repair. Nucleic Acids Res. 1997; 25:992–4.
- Ekwall B, Ekwall K. Comments on the use of diverse cell systems in toxicity testing. Altern Lab Anim. 1988; 15:193–200.
- Balls M, Fentem J. Use of basal cytotoxicity and target organ toxicity tests in hazard identification and risk assessment. Altern Lab Anim. 1992; 20:368–88.
- Shrivastava R, John G, Rispat G, Chevalier A, Massingham R. Can the in vivo maximum tolerated dose be predicted using in vitro techniques? A working hypothesis. Altern Lab Anim. 1991; 19:393–402.
- Cingi MR, De Angelis I, Fortunati E, Reggiani D, Bianchi V, Tiozzo R, et al. Choice and standardization of test protocols in cytotoxicology: A multicentre approach. Toxicol In Vitro. 1991; 5:119–25.
- Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65:55–63.
- Department of Environment, Environmental Quality (Industrial Effluent) Regulation 2009. Standard B, Environmental Quality Act; 1974
- Hussain J, Hussain I, Arif M. Characterization of textile wastewater. J Ind Pollut Control. 2004; 20:137–44.
- Srebrenkoska V, Zezova S, Spasova S, Golomeova S. Methods for waste waters treatment in textile industry. International Scientific Conference UNITECH; 2014.
- Klemola K, Honkalampi-Hamalainen U, Liesivuori J, Pearson J, Lindstrom-Seppa P. Evaluating the toxicity of reactive dyes and fabrics with the spermatozoa motility inhibition test. AUTEX Research Journal. 2006; 6–3.
- Henderson L, Wolfreys A, Fedyk J, Bourner C, Widebank S. The ability of comet assay to discriminate between genotoxins and cytotoxins. Environmental Safety Laboratory, Unilever Research. United Kingdom: Oxford University Press; 1998.
- Hartmann A, Kiskinis E, Fjallman A, Suter W. Influence of cytotoxicity and compound precipitation on test results in the alkaline comet assay. Mutat Res. 2001; 497:199–212.
- Olive PL, Banáth JP, Durand RE. Heterogeneity in radiationinduced DNA damage and repair in tumor and normal cells measured using the “comet” assay. Radiat Res. 1990; 122:86–94.
- Ghaly A, Ananthashankar R, Alhattab M, Ramakrishnan V. Production, characterization and treatment of textile effluents: A critical review. J Chem Eng Process Technol. 2014; 5:182.
- Wang C, Yediler A, Lienert D, Wang Z, Kettrup A. Toxicity evaluation of reactive dyestuffs, auxiliaries and selected effluents in textile finishing industry to luminescent bacteria Vibrio fischeri. Chemosphere. 2002; 46:339–44.
- Sharma KP, Sharma S, Sharma S, Singh PK, Kumar S, Grover R, et al. A comparative study on characterization of textile wastewaters (untreated and treated) toxicity by chemical and biological tests. Chemosphere. 2007; 69:48–54.
- Shaikh MA. 2009. Environmental issues related with textile sector. Pakistan Textile Journal. 10: 36–40.