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Singh, Rashmi
- Ameliorative Potential of Quercetin Against Paracetamol-induced Oxidative Stress in Mice Blood
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Authors
Affiliations
1 Departments of Pharmacology, Shri Ram Murti Smarak Institute of Medical Sciences, Bareilly, Uttar Pradesh, 243 202, IN
2 Division of Medicine, Indian Veterinary Research Institute, Izatnagar - 243 122, Bareilly, Uttar Pradesh, IN
3 Departments of Pathology, Shri Ram Murti Smarak Institute of Medical Sciences, Bareilly - 243 202, IN
1 Departments of Pharmacology, Shri Ram Murti Smarak Institute of Medical Sciences, Bareilly, Uttar Pradesh, 243 202, IN
2 Division of Medicine, Indian Veterinary Research Institute, Izatnagar - 243 122, Bareilly, Uttar Pradesh, IN
3 Departments of Pathology, Shri Ram Murti Smarak Institute of Medical Sciences, Bareilly - 243 202, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 18, No 2 (2011), Pagination: 140-145Abstract
The aim of the present study was to evaluate the ameliorative potential of quercetin (QC) against paracetamol (PCM)- induced oxidative stress and biochemical alterations in mice blood. A total of 36 mice were randomly allocated into six groups, six mice in each. Group I served as healthy controls, while groups II and III were administered with N-acetylcysteine (NAC) and QC alone respectively. Group IV was administered with PCM alone. Groups V and VI were administered with PCM on day 0 followed by NAC and QC, respectively, for 6 consecutive days. On day 7th blood samples were obtained and subjected for the assays of oxidative stress and serum biochemical panels. Erythrocytic lipid peroxides contents of alone PCM-intoxicated mice were significantly higher, while reduced glutathione contents were found to be significantly lower in comparison with the healthy controls. The activities of antioxidant enzymes were also found to be singnificantly lower in these mice. Additionally, significantly increased activities of serum aspartate transaminase, alanine transaminase and alkaline phosphatase, as well as levels of bilirubin, urea and creatinine were revealed by these mice. Postadministration with QC remarkably alleviated the over production of MDA and improved GSH levels in PCM-intoxicated mice blood. In addition, antioxidant enzymes; glutathione peroxidase, glutathione-S-transferase, superoxide dismutase and catalase activities were also improved significantly in these mice. QC had also considerably ameliorated the altered biochemical parameters toward normalcy. Thus, it can be concluded that QC may constitute a remedy against PCM-induced oxidative stress and reno-hepatic injuries.Keywords
Antioxidant, lipid peroxidation, oxidative stress, paracetamol, quercetin- Anti-hepatotoxic Potential of Indigofera tinctoria and its isolated Isothiocyanate compound ‘ITC-1’ against NPYR-CCl4 Intoxicated Mice
Abstract Views :275 |
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Authors
Rashmi Singh
1,
Veena Sharma
2
Affiliations
1 Department of Biotechnology and Microbiology, Alpine Group of Management and Technology, Dehradun – 248001, Uttarakhand, IN
2 Department of Bioscience and Biotechnology, Banasthali University, Vanasthali – 304022, Rajasthan, IN
1 Department of Biotechnology and Microbiology, Alpine Group of Management and Technology, Dehradun – 248001, Uttarakhand, IN
2 Department of Bioscience and Biotechnology, Banasthali University, Vanasthali – 304022, Rajasthan, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 26, No 1&2 (2019), Pagination: 30-36Abstract
Isothiocyanate derivative from hydroethanolic extract of Indigofera tinctoria (HEIT) was previously isolated, purified and characterized as 1-[1,2-Diisothiocyanato-2-(3-isothiocyanato-2,2-dimethyl-propylsulfanyl)-ethoxy]-3-isothiocyanato-2,2-dimethyl-propane (C16H22N4OS5; m/z 446.70; ITC-1). To elucidate hepatoprotective efficacy, liver toxicity was induced in mice via intoxication of N-Nitrosopyrrolidine (NPYR) followed by CCl4 for 50 days. Both low and high doses of crude HEIT were given orally to NPYR treated mice for 21 days. Silymarin was also administered to compare the results. After completion of post treatment, various hepatic toxicity markers were evaluated. Results showed that both doses of HEIT and ITC-1 have successfully normalized the levels of AST, ALT (P<0.001 v/s NPYR treated group), ALP and bilirubin (P<0.01). ITC-1 has showed better remedial response against liver toxicity in comparison to Silymarin. Thus, we concluded that both I. tinctoria and ‘ITC-1’ have future remedial aspect in diminution of hepatic toxicity.Keywords
AST, Bilirubin, Hepatic Toxicity, Indigofera tinctoria, Isothiocyanate Compounds, Silymarin.References
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