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Srivastava, Prachi
- Identification of Limiting Factors for the Optimum Growth of Fusarium oxysporum in Liquid Medium
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1 Amity Institute of Biotechnology, Amity University Uttar Pradesh (Lucknow Campus), Lucknow - 226 010, IN
2 Department of Biotechnology, Integral University, Lucknow - 201 126, Uttar Pradesh, IN
1 Amity Institute of Biotechnology, Amity University Uttar Pradesh (Lucknow Campus), Lucknow - 226 010, IN
2 Department of Biotechnology, Integral University, Lucknow - 201 126, Uttar Pradesh, IN
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Toxicology International (Formerly Indian Journal of Toxicology), Vol 18, No 2 (2011), Pagination: 111-116Abstract
Fusarium oxysporum is a highly ubiquitous species that infects a wide range of hosts causing various diseases such as vascular wilts, yellows, rots, and damping-off. Despite the immense economic significance of this phytopathogen, few workers have reported growth studies in this genus in submerged culture. In the present study, several parameters such as change in media pH, biomass, pattern of substrate utilization, viability of the fungal cells, and protein content were observed over a period of time. The fungal biomass increased at a slow rate for the initial 48 h and thereafter increased at an exponential rate. However, after about 8 days the rapid growth stabilized and the trend became more toward stationary phase. The concentration of glucose in the liquid media decreased rapidly up to the initial 4 days, followed by a slow decrease. The pH of the medium gradually decreased as the fungal growth progressed, the reduction being more pronounced in the initial 48 h. This study would be of immense importance for utilization of F. oxysporum for diverse applications because we can predict the growth pattern in the fungus and modulate its growth for human benefit.Keywords
Biomass production, Fusarium, glucose utilization, MTT assay, protein
- Significant Analysis of Microarray (SAM) to Identify Synergistic Effect of RV and NGF in Repairing Damaged Neuronal Cells
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Authors
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1 Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Lucknow - 226028, Uttar Pradesh, IN
1 Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Lucknow - 226028, Uttar Pradesh, IN
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Toxicology International (Formerly Indian Journal of Toxicology), Vol 25, No 1 (2018), Pagination: 26-39Abstract
Neurodevelopmental disorders include diseases that are related with genetic disorders and are caused due to stress condition or environmental toxins during pre or post natal condition. In recent years, there is considerable research in neurodevelopmental disorders and examining therapeutic role of resveratrol as potential antioxidant. There is less information about the genes that are mutated or altered during neurodevelopmental phase and cause neuronal developmental disorders. Current researches are evidentially showing the therapeutic potential of Resveratrol (RV) and Nerve Growth Factor (NGF) against neuronal diseases. In current study Microarray experiment was designed to identify the genes that are altered when Mesenchymal Stem Cells (MSC) were exposed to Monocrotophos (MCP). MSCs were also coexposed with resveratrol and nerve growth factor to study the synergistic effect of NGF with RV. Computational analysis of microarray data was carried out through different software’s and bioinformatics tools to identify genes that are expressed in different samples across microarray experiment. Statistical methods like T-test, SAM analysis and clustering techniques were performed between different samples using MeV software. Through Significant Analysis of Microarray (SAM) method we identified positive and negative significant genes with respect to current study. Clustering method was used to cluster genes associated with neuronal disease genes. Key genes that were predicted on the basis of t-test and SAM analysis are DNMT1, PGAP1, RDX and PEX26 genes, these genes have noticeable function in different classes of neuronal diseases like cerebellar ataxia, deafness, narcolepsy, mental retardation and zellweger spectrum disorder etc. Cluster analysis we identified genes such as ATP6V0D1, TESMIN, TRIM22, NAPEPLD, CDK7 and PKM, these genes have important function in cell growth, cell proliferation, protein synthesis, cell cycle regulation, and cell signaling. Study suggests that exposure of damaged neuronal cells to RV and NGF enhances the expression of neuronal repair genes, thus signifies the neuroprotectant and synergistic activity of RV and NGF. “The data discussed in this publication have been deposited in NCBI’s Gene Expression Omnibus database and are accessible through GEO Series accession number GSE121261 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE121261).”Keywords
Microarray Analysis, Nerve Growth Factor, Neuronal Damage, Neuronal Diseases, Resveratrol, SAM.References
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- In-silico Characterization and 3D Structure Prediction of MX Protein of Lates Calcarifer (Barramundi): A Major Threat to Aqua Industry
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Affiliations
1 Amity Institute of Biotechnology, Amity University, Lucknow - 226028, Uttar Pradesh, IN
2 ICAR- Central Institute of Fisheries Education, Versova, Mumbai - 400061, Maharashtra, IN
3 National Bureau of Fish Genetic Resources (Indian Council of Agricultural Research), Canal Ring Road, P.O. Dilkusha, Lucknow - 226002, Uttar Pradesh, IN
1 Amity Institute of Biotechnology, Amity University, Lucknow - 226028, Uttar Pradesh, IN
2 ICAR- Central Institute of Fisheries Education, Versova, Mumbai - 400061, Maharashtra, IN
3 National Bureau of Fish Genetic Resources (Indian Council of Agricultural Research), Canal Ring Road, P.O. Dilkusha, Lucknow - 226002, Uttar Pradesh, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 25, No 4 (2018), Pagination: 232-239Abstract
Betanoda virus is one of the most important and emerging groups of viruses known to infect around 40 species found to be worldwide in distribution. The most common and virulent target of infection for this virus is (Lates Calcarifer) (barramundi). It is found that the expression of MX protein is found to be the more susceptible reason for this viral infection. Considering this current study including characterization to structure prediction revolves around the MX protein as a target. The progression of this study describes the amino acid sequence of MX protein was retrieved from UniProt database in Fasta format and further primary structure analysis and characterization including nature of amino acids, instability index reading, GRAVY, determination of phosphorylation as well as signal peptide cleavage sites was done with the help of various tools. Secondary structure prediction has proceeded through SOPMA server analysis revealed that MX protein has mixed secondary structure, i.e., mostly alpha-helix and beta-turn. The progression of this work prediction of a 3D structure along with functional site prediction of MX protein of Fish (Lates Calcarifer) is done through standard modeling tools. The 3D structure of this protein of (Lates Calcarifer) as documented in this study may provide a valuable aid for designing an inhibitor or better ligand against viral nervous necrosis disease and could play a vital role in drug design.Keywords
Barramundi, Betanoda Virus, Ligand, MX Protein, Viral Nervous Necrosis.References
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