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Nariya, Mukesh
- Assessment Of The Genotoxic Potential Of Rasamanikya, An Ayurvedic Arsenical Formulation In Albino Rats
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1 Department of Rasa Shastra and Bhaisajya Kalpana, ITRA, Jamnagar – 361001, Gujarat, IN
2 Pharmacology Department, ITRA, Jamnagar – 361001, Gujarat, IN
1 Department of Rasa Shastra and Bhaisajya Kalpana, ITRA, Jamnagar – 361001, Gujarat, IN
2 Pharmacology Department, ITRA, Jamnagar – 361001, Gujarat, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 29, No 2 (2022), Pagination: 159-172Abstract
Shodhita Haratala (processed orpiment) is a single ingredient of Rasamanikya (RM). Arsenicals are known for producing toxic effects in humans, if not processed as per classical text therefore, the present study was aim to generate data for the safety of RM in albino rats. In the present study, two samples of RM were prepared by using two different purification media of orpiment, i.e., juice of Benincasa hispida (Thunb.) Cong. and lime water. Extract of Tinospora cordifolia Linn. (GG) was used as an adjuvant. Both the samples of RM along with GG were administered to the albino rats for 60 consecutive days at the therapeutic dose, TED (90 mg/kg) and TED×5 (450 mg/kg) orally in albino rats. Sample of RM along with GG not having the potential to produce any mutagenic or genotoxicity effects in albino rats. Further, there is no significant difference found in the safety of the orpiment on changing of media for purification.Keywords
Chromosomal Aberration, Genotoxicity, Orpiment, Rasamanikya, Safety.References
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- Acute and 90 Days Repeated Dose Toxicity Of Rasa Parpati (an Ayurvedic Mercurial Formulation) In Charles Foster Albino Rats
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Authors
Affiliations
1 Ayush Wing, Guna – 473001, Madhya Pradesh, IN
2 Department of Rasashastra and Bhaishajya Kalpana, ITRA, Jamnagar – 361008, Gujarat,, IN
3 Pharmacology Laboratory, ITRA, Jamnagar – 361008, Gujarat,, IN
4 4Department of Rasashastra and Bhaishajya Kalpana, All India Institute of Ayurveda, New Delhi – 110076,, IN
1 Ayush Wing, Guna – 473001, Madhya Pradesh, IN
2 Department of Rasashastra and Bhaishajya Kalpana, ITRA, Jamnagar – 361008, Gujarat,, IN
3 Pharmacology Laboratory, ITRA, Jamnagar – 361008, Gujarat,, IN
4 4Department of Rasashastra and Bhaishajya Kalpana, All India Institute of Ayurveda, New Delhi – 110076,, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 27, No 3&4 (2020), Pagination: 149-157Abstract
Rasa parpati is a unique Ayurvedic mercurial formulation extensively prescribed by Ayurvedic physicians to treat different ailments. Considering toxicity concerns of traditional formulations for containing heavy metals like Mercury, Arsenic, Lead etc.; it becomes, imperative to evaluate safety aspects of such formulations. In Ayurveda, metals and minerals are advocated to be processed through the specified guidelines before their therapeutic application. An attempt has been made to evaluate the impact of Shodhana on safety aspects of Rasa parpati through acute and 90 days repeated dose toxicity of Rasa parpati that was prepared by two different samples of raw materials. One by using Hingulottha parada and Shuddha gandhaka (HRP) while, another with Ashuddha parada and Ashuddha gandhaka (ARP). Oral acute toxicity of test drugs was carried out at limit dose of 2000 mg/kg orally along with adjuvant in Charles foster female rats. In 90 days repeated dose toxicity, both ARP and HRP were orally administered at therapeutically equivalent dose (22.5 mg/kg), TEDx5 (112.5 mg/kg), TEDx10 (225 mg/kg) levels along with adjuvant for 90 days. Two recovery groups were also studied in the experiment. Acute toxicity revealed that, both the test drugs did not produce any signs and symptoms of toxicity and mortality up to oral dose of 2000 mg/kg in rats. Administration of HRP for 90 days revealed that, the drug at higher dose has potential to produce liver and kidney toxicity however, can be relatively safe at therapeutic dose level. ARP has potential for liver, kidney and GI tract toxicity with deposition in other organs. The recovery study suggests that, after discontinuation of HRP the observed changes are reversible in nature while, in ARP some of the observed changes are irreversible in nature. From present study, it is concluded that HRP was found to be safer than ARP at different dose levels in rats suggesting safe use at therapeutic dose level. It proves that classical procedures like Shodhana are mandatorily to be followed in the pharmaceutical processing of these formulations.Keywords
Ayurveda, Mercury, Parada, Rasa parpati, Safety, Toxicity.References
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