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Bhardwaj, Jitender Kumar
- Potential of Zingiber officinale Extract against Chlorpyrifos Toxicity in Caprine Testicular Cells
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Authors
Affiliations
1 Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, IN
2 Department of Biochemistry, Sri Venkateshvara College, University of Delhi, Delhi – 110021, IN
3 Department of Library and Information Science, Kurukshetra University, Kurukshetra – 136119, Haryana, IN
1 Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, IN
2 Department of Biochemistry, Sri Venkateshvara College, University of Delhi, Delhi – 110021, IN
3 Department of Library and Information Science, Kurukshetra University, Kurukshetra – 136119, Haryana, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 29, No 4 (2022), Pagination: 465-473Abstract
Chlorpyrifos, an organophosphate insecticide, is currently under studies due to its reproductive toxicity in non-targeted organisms. The present study on its efficacy as reproductive toxicant, revealed its effect on testicular cells of caprines. CPF was found to decrease cell viability in testicular cells, induced histo-architectural alterations like loss of tubular interactions and empty luminal spaces in testis; initiated DNA damage associated apoptosis as revealed by MTT, Histology and EB/AO fluorescence assay respectively, at both the doses (10 and 50 μM). CPF mediated toxicity induced oxidative stress in testicular cells as revealed by dose- and time-dependent increase in lipid peroxidation and decline in ferric reducing antioxidant power. However, supplementation of Zingiber officinale (ginger) extract reduced the CPF mediated decrease in cell viability, histological defects, incidence of apoptosis, and generated oxidative stress parameters; thus, proving its potential as a therapeutic agent against CPF, preventing testicular cells toxicity mediated reproductive disorders.Keywords
Apoptosis, Chlorpyrifos, Testicular Cells, Zingiber officinale.
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