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Vemu, Bhaskar
- Evaluation of Aldrin-Induced Oxidative Stress and Apoptosis in J774 Macrophages
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1 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, IN
1 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 22, No 2 (2015), Pagination: 88-96Abstract
The present study was carried out to evaluate the aldrin-induced oxidative stress and apoptosis in murine macrophage (J774A.1) cells. Acute exposure of aldrin for 2 hrs was given to the J774A.1 cells cultured in DMEM with 10% FBS at 37oC and 5% CO2 in air with 95% relative humidity under in vitro system. Lethal concentration-50 (LC50) was calculated after exposure period as 7.24 μg/ml. Further, cells were exposed with three different concentration of aldrin (1.81, 3.65 and 7.24 μg/ml) and 0.1% DMSO was used as negative control. The antioxidant enzymes and non-enzymes were determined along with G6pDH, LDH and ALP enzymes in J774A.1 cells. Cells were monitored for cell morphology and apoptosis. Exposure of aldrin to J774A.1cells resulted in increase in lipid peroxidation and decrease in antioxidant enzyme/ nonenzymes system. Further it caused decrease in G6PDH enzymes activity and excess leakage of LDH and ALP enzymes. The aldrin-treated cells showed higher number of apoptotic cells with alteration in cell morphology indicating apoptotic and necrotic changes. These effects were noticed in dose dependant manner. In conclusion, the result of in vitro study suggests that the aldrin can induced the process of apoptosis and cell death through the generation of ROS and thereof oxidative insult in J774A.1 cells.Keywords
Aldrin, Apoptosis, Antioxidant Enzymes, J774A.1 Cells, Oxidative Stress.
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- Amelioration of Quinalphos-Induced Oxidative Stress by Emblica officinalis Fruit Extracts in Rats
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Authors
Affiliations
1 Department of Veterinary Pharmacology and Toxicology, CSKHPKV, Palampur, IN
2 Department of Veterinary Pharmacology and Toxicology, GADVASU, Ludhiana, IN
3 Department of Veterinary Pathology, GADVASU, Ludhiana, IN
1 Department of Veterinary Pharmacology and Toxicology, CSKHPKV, Palampur, IN
2 Department of Veterinary Pharmacology and Toxicology, GADVASU, Ludhiana, IN
3 Department of Veterinary Pathology, GADVASU, Ludhiana, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 24, No 3 (2017), Pagination: 301-308Abstract
The fruit of Emblica officinalis (commonly known Amla) contain potent antioxidants and is most commonly used in the Ayurveda for the treatment of various diseases. Quinalphos- a organophorus insecticide is known to alter antioxidant defense system in mammals. With this premise, experiment was planned with objective to study the effect of amla fruit extract supplementation on quinalphos induced oxidative stress. Twenty-four Sprague-Dawley rats were homogenously divided into six groups of four animals each. Group I was kept as control. Group II was orally given quinalphos at the dose rate of 2 mg.kg-1. day-1. Group III and V were orally gavaged with amla in sugar and honey bases respectively at the dose rate of 50 μg.kg-1.day-1 whereas group IV and VI were orally dosed with quinalphos along with amla in sugar and honey bases respectively at above mentioned dose rates from day 16-45. The various indices of antioxidant status were analyzed in major organs of treatment groups viz. Liver, kidney and brain. Sub chronic quinalphos administration produced oxidative stress in rats as evidenced by increase in lipid peroxidation, glutathione peroxidase and decline in superoxide dismutase. Oral supplementation of amla extract in sugar and honey bases provided partially amelioration against altered antioxidant status following sub chronic quinalphos exposure. Results of histopathological study were also in corroboration with blood antioxidant status validating amla in either bases was unable to provide full protection against quinalphos induced toxicity in rats.References
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Authors
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1 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, IN
1 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, IN