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Hazra, Alok Kumar
- Risk of Betel Nut Chewing: Chemical and Toxicological Studies
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Authors
Affiliations
1 Department of Pharmacology, Institute of Post Graduate Medical Education & Research, Kolkata, IN
2 Department of Pharmacology, R.G. Kar Medical College, Kolkata, IN
3 Division of Phytochemistry, RKMA Quality Testing Laboratory (AYUSH), Narendrapur, Kolkata, IN
1 Department of Pharmacology, Institute of Post Graduate Medical Education & Research, Kolkata, IN
2 Department of Pharmacology, R.G. Kar Medical College, Kolkata, IN
3 Division of Phytochemistry, RKMA Quality Testing Laboratory (AYUSH), Narendrapur, Kolkata, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 24, No 1 (2017), Pagination: 39-45Abstract
Last few decades epidemiological reports warn that the development of oral submucous fibrosis − a precancerous state, is universal among betel nut or areca nut users. Betel nut contains arecoline and other microelements which are released into the buccal cavity during the process of areca nut chewing. The present effort was undertaken to find out the correlations between chemical ingredients, like arecoline, magnesium, iron and copper present in betel nut with toxicological incidences in experimental animals. Arecoline was estimated by HPLC (Perkin Elmer, USA) and other micronutrients like magnesium, iron and copper was assessed by AAS (Perkin Elmer, USA). Repeated dose 90-day sub-acute oral toxicity of betel nut water extract (BNE) at the dose of 0.5X10-2 ml/g/day and 1.0X10-2 ml/g/day was performed on Wistar rats as per the Organization for Economic Co-operation and Development (OECD) guidelines No. 408. The result indicates that betel nut contains Group 1 human carcinogen arecoline with significant high level of magnesium, iron and copper. Moreover, consumption of betel nut induces liver damage in rats by indicating significant enhancement of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP). It may conclude that chemical ingredients presences in betel nut are harmful for health and it should be restricted.Keywords
Areca Nut, Arecoline, Alkaloid, Fibrosis, HPLC, Toxicity.
References
- Amudhan MS, Begum VH, Hebbar KB. A review on phytochemical and pharmacological potential of Areca catechu L. seed. Int J Pharma Sci Res 2012; 3:4151-4157.
- Miranda CMD, van Wyk CW, van der Biji P, Basson NJ. The effect of areca nut on salivary and selected oral microorganisms. Int Dent J 1996; 46: 350-356.
- Gupta PC, Ray CS. Epidemiology of betel quid usage. Ann Acad Med Singapore 2004; 33:31-36.
- Nair U, Bartsch H, Nair J. Alert for an epidemic of oral cancer due to use of the betel quid substitutes gutkha and pan masala: a review of agents and causative mechanisms. Mutagenesis 2004; 19:251-262.
- Lan TY, Chang WC, Tsai YJ, Chuang YL, Lin HS, Tai TY. Areca nut chewing and mortality in an elderly cohort study. Am J Epidemiol 2007; 165:677-683.
- Lord GA, Lim CK, Warnakulasuriya S, Peters TJ. Chemical and analytical aspects of areca nut. Addict Biol 2002; 7: 99102.
- IARC. International Agency for Research on Cancer (IARC) – Summaries & Evaluations: Betel-quid and arecanut chewing. IARC Monogr Eval Carcinog Risk Chem Hum 85:39, Lyon: IARC, 2004.
- Chatterjee A, Deb S. Genotoxic effects of arecoline either by the peritoneal or oral route in murine bone marrow cells and the influence of N-acetylcysteine. Cancer Lett 1999; 13:23-31.
- Chiang S, Jiang S, Wang Y, Chiang HC, Chen PH, Tu HP. Characterization of arecoline-induced effects on cytotoxicity in normal human gingival fibroblasts by global gene expression profiling. Toxicol Sci 2007; 100:66-74.
- Scutt A, Meghji S, Canniff JP, Harvey W. Stabilisation of collagen by betel nut polyphenols as a mechanism in oral submucous fibrosis. Experimentia 1987; 43:391-393.
- Trivedy C, Meghji S, Warnakulasuriya KA, Johnson NW, Harris M. Copper stimulates human oral fibroblasts in vitro: a role in the pathogenesis of oral submucous fibrosis. J Oral Pathol Med 2001; 30:465-470.
- Adhikari A, De M. Toxic effects of betal quid. Int J Hum Genet 2013; 13:7-14.
- Wintstock AR. Areca nut - abuse liability, dependence and public health. Addict Biol 2002; 7: 133-138.
- Ghayur MN, Kazim SF, Rasheed H, Khalid A, Jumani MI, Choudhary MI, Gilani AH. Identification of antiplatelet and acetylcholinesterase inhibitory constituents in betel nut. Zhong Xi Yi Jie He Xue Bao 2011; 9:619-625.
- CPCSEA guidelines for laboratory animal facility. Committee for the Purpose of Control and Supervision of Experiments on Animals. Indian J Pharmacol 2003; 35: 257-274.
- The Ayurvedic Pharmacopeia of India. Vol 1, Department of AYUSH, Ministry of India, New Delhi, India, 2004.
- APHA Method. Revisions to Standard Methods for the Examination of Water and Wastewater. (Supplement). American Public Health Association, Washington DC, 2001.
- Adhikari A, Hazra AK, Sur TK. Detection of arecoline by simple HPTLC method in Indian non-tobacco pan masala. J Adv Pharmaceut Tech Res 2015; 6:195-199.
- OECD Guideline for the testing of chemicals. Acute oral toxicity in animals. OECD/OCDE No. 423, Adopted 2014.
- OECD Guideline for the testing of chemicals. Repeated dose 90-day oral toxicity study in rodents. OECD/OCDE No. 408, Adopted 1998.
- Sur TK, Chatterjee S, Hazra AK, Pradhan R, Chowdhury S. Acute and sub-chronic oral toxicity study of black tea in rodents. Indian J Pharmacol 2015; 47:167-172.
- Tadakamadla J, Kumar S, GP M. Evaluation of serum copper and iron levels among oral submucous fibrosis patients. Med Oral Patol Oral Cir Bucal 2011; 16:870-873.
- Shih YU, Chen PS, Wu CH, Tseng YT, Wu YC, Lo YC. Arecoline, a major alkaloid of the areca nut, causes neurotoxicity through enhancement of oxidative stress and suppression of antioxidant protective system. Free Rad Biol Med 2010; 49:1471-1479.
- Lohith TS, Shridhar NB, Dilip SM, Pattar J, Suhasini K. Repeated dose 28-day oral toxicity study of raw areca nut extract in rats. Int Res J Pharm 2013; 4:238-240.